Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation

Singh, Mahipal; Sharma, Jai Gopal; Chakrabarti, Rina

doi:10.1016/j.jphotobiol.2015.05.019

Cited by 20 publications

(7 citation statements)

References 55 publications

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“…In the present study, NOS level was significantly reduced at different doses of UV-B radiation on spawn and fry of C. batrachus compared to control. Similar observation was reported in the larvae of Catla catla exposed to different doses of UV-B radiation, where nitric oxide synthase (NOS) level was reduced compared to control (Singh et al, 2015). NOS level of rohu larvae was also affected by UV-B radiation (Singh et al, 2013).…”

Section: Discussionsupporting

confidence: 80%

“…Increased level of CP in this study was due to increase of ROS induced by ultraviolet radiation, because it is the oxidation product of protein caused by ROS and in this case reactive ketones and reactive aldehydes were formed and regulated by DNPH (2,4dinitrophenylhydrazine) to form hydrazones (Stadtman and Levine, 2000;Stadtman, 2001;Levine, 2002;Moskovitz and Oien, 2010). Similar relationship was also observed between the dose of UV-B and the CP level, where CP level was significantly (p< 0.05) higher in the larvae of Catla catla exposed at the dose 1512 (15 min) mJ/cm 2 UV-B compared to the control group (Singh et al, 2015). A significant increase in protein carbonyls was also observed in the organs, viz., liver, kidney and gill in fish (Channa punctata) exposed to different toxic compounds (Parvez and Raisuddin, 2005).…”

Section: Discussionsupporting

confidence: 73%

“…Nitric oxide synthase (NOS) of the sample was measured as per the method (Singh et al, 2015). The tissue sample (100 mg) was homogenized in 1 ml of phosphate buffer saline (pH 7.4) and centrifuged at 10,000 rpm for 10 min at 4 C. 100 μl of supernatant was mixed with equal volume of Griess reagent (1% of sulfanilamide and 0.1% of naphthylethylenediamine in 5% of phosphoric acid) and incubated at room temperature for 10 min.…”

Section: Nitric Oxide Synthase (Nos)mentioning

confidence: 99%

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Evaluation of Oxidative Stress Enzymes of Spawn and Fry of Clarias batrachus (Linn. 1758) Exposed to Artificial UV-B Radiation: A Biomarker Analysis

Mandal¹,

Subhas²,

Patra³

et al. 2021

IJZI

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The present study investigated the impact of the artificial exposure of UV-B radiation, based on different time span and intervals, on spawn and fry of Clarias batrachus at the level of oxidative stress enzymes. The spawn and fry of C. batrachus were exposed for time span of 5 and 10 days at a duration of 5, 10 and 15 min per day. The specific growth rate (SGR%), weight gain (%WG) and oxidative stress enzymes, i.e., superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), protein carbonyl (CP), nitric oxide synthase (NOS) and trypsin were analysed. The specific growth rate and the per cent weight gain of spawn and fry of C. batrachus were decreased as compared to control. The results of the oxidative stress enzymes, viz., SOD, CAT, GSH and NOS were decreased in both spawn and fry of C. batrachus in comparison to control. CP values were increased for both the spawn and fry as compared to control. Trypsin in spawn and fry decreased gradually. This study has been able to explore the impact of UV -B exposure on spawn and fry of C. batrachus in regard to these oxidative stress enzymes, which can be established as biomarkers in monitoring the UV-B radiation pollution. Finally, it infers that the harmful radiations may cause deterioration in fish health as well as yield, so, UV-B radiation becomes a major concern to the aquaculture industry, especially, with regard to sustainable fish production.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 73%

Section: Nitric Oxide Synthase (Nos)mentioning

confidence: 99%

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Evaluation of Oxidative Stress Enzymes of Spawn and Fry of Clarias batrachus (Linn. 1758) Exposed to Artificial UV-B Radiation: A Biomarker Analysis

Mandal¹,

Subhas²,

Patra³

et al. 2021

IJZI

View full text Add to dashboard Cite

show abstract

“…Several reports have shown that UV induces production of reactive oxygen species, photo-oxidation, and increase in the oxidative damage to biomolecules [46][47][48] . Earthworm, Lumbricus terrestris secretes a photoreactive sterol and produces singlet following UV-A exposure 49 .…”

Section: Discussionmentioning

confidence: 99%

Exploring the effect of UV-C radiation on earthworm and understanding its genomic integrity in the context of H2AX expression

Chelladurai

Christyraj

Azhagesan

et al. 2020

Sci Rep

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Maintaining genomic stability is inevitable for organism survival and it is challenged by mutagenic agents, which include ultraviolet (UV) radiation. Whenever DNA damage occurs, it is sensed by DNA-repairing proteins and thereby performing the DNA-repair mechanism. Specifically, in response to DNA damage, H2AX is a key protein involved in initiating the DNA-repair processes. In this present study, we investigate the effect of UV-C on earthworm, Perionyx excavatus and analyzed the DNA-damage response. Briefly, we expose the worms to different doses of UV-C and find that worms are highly sensitive to UV-C. As a primary response, earthworms produce coelomic fluid followed by autotomy. However, tissue inflammation followed by death is observed when we expose worm to increased doses of UV-C. In particular, UV-C promotes damages in skin layers and on the contrary, it mediates the chloragogen and epithelial outgrowth in intestinal tissues. Furthermore, UV-C promotes DNA damages followed by upregulation of H2AX on dose-dependent manner. Our finding confirms DNA damage caused by UV-C is directly proportional to the expression of H2AX. In short, we conclude that H2AX is present in the invertebrate earthworm, which plays an evolutionarily conserved role in DNA damage event as like that in higher animals.

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“…The supplementation of seeds of A. aspera in diet also enhances the growth of fish. Even it protects the early larvae of carps from harmful UV-B irradiation [14, 15, 16, 17, 18].…”

Section: Introductionmentioning

confidence: 99%

Validation of growth enhancing, immunostimulatory and disease resistance properties of Achyranthes aspera in Labeo rohita fry in pond conditions

Kumar

Sharma

Singh

et al. 2019

Heliyon

Self Cite

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The immunostimulatory and disease resistance properties of herb Achyranthes aspera L. (Amaranthaceae) were evaluated in rohu Labeo rohita in pond. Rohu fry (1.9 ± 0.08 g) were cultured in hapas (25 hapa −1 ) set inside a pond and were fed with two experimental diets containing 0.5% seeds (D1) and leaves (D2) of A. aspera and control diet (D3). Fish were challenged with Aeromonas hydrophila after 80 days. The cumulative mortality rate of fish was significantly ( P < 0.05) higher in D3 (28–48%) compared to others. Average weight was significantly ( P < 0.05) higher in D1 (6.5–12.5%) compared to other treatments. Myeloperoxidase and nitric oxide synthase levels were significantly ( P < 0.05) higher in D1 and D2 compared to D3. Thiobarbituric acid reactive substances and carbonyl protein levels were significantly ( P < 0.05) lower in hepatopancreas and kidney of D1 compared to others. In hepatopancreas, the expressions of lysozyme C, loysozyme G, TNF-α, IL-10 and IL-1β were significantly ( P < 0.05) higher in D1 compared to others. This treatment was followed by D2. In kidney, lysozyme G and TNF-α levels were significantly ( P < 0.05) higher in D1 and D2 compared to D3. Whereas, IL-10 and IL-1β were significantly ( P < 0.05) down-regulated and up-regulated, respectively in kidney of D2. There was up-regulation ( P < 0.05) of TLR-4 in hepatopancreas and kidney of D1 and D2 diets fed rohu, respectively compared to others.

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Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation

Cited by 20 publications

References 55 publications

Evaluation of Oxidative Stress Enzymes of Spawn and Fry of Clarias batrachus (Linn. 1758) Exposed to Artificial UV-B Radiation: A Biomarker Analysis

Evaluation of Oxidative Stress Enzymes of Spawn and Fry of Clarias batrachus (Linn. 1758) Exposed to Artificial UV-B Radiation: A Biomarker Analysis

Exploring the effect of UV-C radiation on earthworm and understanding its genomic integrity in the context of H2AX expression

Validation of growth enhancing, immunostimulatory and disease resistance properties of Achyranthes aspera in Labeo rohita fry in pond conditions

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