Uzun Gün Şartlarında Patates (Solanum tuberosum L.)’in Mikroçoğaltımında Jasmonik Asidin Etkisi

Abstract

Meristem derived in vitro plantlets of potato (Solanum tuberosum L.) cultivars Pasinler, Granola, and Caspar were micropropagated on agar-solidified MS medium containing different concentrations of jasmonic acid (JA) using single-node stem cuttings. The results of the study showed that inclusion of 1.0 µM JA in the MS medium provided a substantial and visible increase in most of the plantlet characteristics studied and the effect of this concentration was more pronounced compared to the control and all other JA concentrations. The highest number of shoots (8.00), nodes (19.00), leaves (19.00), and roots (15.75) on cv. Caspar and a maximum shoot length (15.13 cm) was observed on cv. Granola from 1.0 µM JA concentration. This concentration of JA also helped to induce longest roots (14.95 cm) on cv. Pasinler. The highest plantlet fresh weight from cv. Pasinler (649.38 mg), and cv. Caspar (630.18 mg), and maximum dry weight of plantlets from cv. Pasinler (98.58 mg), cv. Caspar (93.89 mg) and cv. Granola (79.03 mg) were noted on MS medium containing 1.0 µM of JA. Since the application of JA accelerates the multiplication of young plantlets throughout the year without depending on the season, this allows fast commercial propagation of new potato cultivars. These results may also serve as basis for the mass production of these economically important potato cultivars through in vitro micropropagation techniques and microtuberization studies.