1994
DOI: 10.1002/jcb.240560407
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V‐sis induces Egr‐1 expression by a pathway mediated by c‐Ha‐ras

Abstract: The early growth response gene, Egr-1, is up-regulated transiently by mitogens and many other stimuli in all cells tested. Using NIH3T3 cells conditionally expressing v-sis from a metallothionein promoter, we show that the addition of Zn2+ stimulates the production of PDGF-B (v-sis) and elicits the expression of Egr-1 in a dose-dependent and time-regulated manner. The signal is likely independent of protein kinase C, but depends on tyrosine kinase and other kinase activities and is mediated by c-Ha-Ras since t… Show more

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Cited by 15 publications
(19 citation statements)
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“…Slow-migrating forms of Egr-1 can be detected as long as 12 h after UV irradiation (data not shown). Our previous measurements of the induction of Egr-1 in NIH3T3 cells by serum or by TPA gave 7 ± tenfold increase in Egr-1 protein while the levels of slow-migrating phosphorylated Egr-1 were not increased by serum and only slightly increased by TPA (Huang et al, 1994b;. The results here suggest that UV significantly enhances both the Egr-1 protein level and its phosphorylation in NIH3T3 cells.…”
Section: Egr-1 Is Phosphorylated In Response To Uv Irradiationsupporting
confidence: 60%
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“…Slow-migrating forms of Egr-1 can be detected as long as 12 h after UV irradiation (data not shown). Our previous measurements of the induction of Egr-1 in NIH3T3 cells by serum or by TPA gave 7 ± tenfold increase in Egr-1 protein while the levels of slow-migrating phosphorylated Egr-1 were not increased by serum and only slightly increased by TPA (Huang et al, 1994b;. The results here suggest that UV significantly enhances both the Egr-1 protein level and its phosphorylation in NIH3T3 cells.…”
Section: Egr-1 Is Phosphorylated In Response To Uv Irradiationsupporting
confidence: 60%
“…For serum stimulation, cells were incubated in DME containing 0.5% CS for 24 h and then 20% serum was added. For all types of stimulation, cells were harvested 24 h after transfection, lysed and assayed for CAT activity as described earlier (Huang et al, 1994b).…”
Section: Cat Assaymentioning
confidence: 99%
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“…When growth factors or other stimuli lead to mitogenesis in cells, Egr-1 is rapidly induced in a pathway via Ras and MAP kinase activation (Huang et al, 1994b(Huang et al, , 1996. There was little variation in Erk-1 MAPK activity in three of the cell lines, with a small increase in H4 cells that expressed wt p53, 2 h after UV-C irradiation (Figure 6b).…”
Section: Apoptosis In P53 Expressing Cellsmentioning
confidence: 99%