V�rification de l'efficacit� d'une guillotine multilames pour la pr�paration standardis�e de tranches tissulaires

Ganguillet, F; Mirkovitch,; Jw, Robinson; Gomba, S

doi:10.1007/bf00587323

Cited by 12 publications

(2 citation statements)

References 4 publications

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“…Indeed, adrenalectomy has been shown to maintain a sustained level of renin release despite a depletion of juxtaglomerular cells in secretory granules. 15 In conclusion, this work has shown an important role for the sodium concentration in the incubation medium. Moreover, the data point to an inhibitory effect of o-adrenergic receptors on renin release.…”

Section: Table 4 Effect Of Propranolol On Renin Release In Vitro Stimmentioning

confidence: 54%

“…The kidneys were divided sagittally into two parts with a razor blade and then cut transversely into slices 0.5 mm thick with the aid of a multiblade guillotine. 15 Two slices (50-70 mg of fresh tissue) were placed in each incubation flask and preincubated in a Gallenkamp incubator for 30-60 minutes at 37°C in 15 ml of Krebs-Ringer bicarbonate solution (140 mM NaCl, 3.2 mM KC1, 2.5 mM CaCl,, 1.3 mM KH,PO 4 , 1.5 mM MgSO 4 , 0.15% glucose, and 0.5% bovine serum albumin, at pH 7.4). A gas mixture of 95% O t -5% CO, was gassed into each medium.…”

Section: Incubation Of Kidney Slicesmentioning

confidence: 99%

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Renin release by rat kidney slices incubated in vitro. Role of sodium and of alpha- and beta-adrenergic receptors, and effect of vincristine.

Capponi¹,

Vallotton²

1976

Circulation Research

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SUMMARYThe role of sodium concentration, of a-and /3-adrenergk receptors, and of a microtabolar inhibitor (vincristine) on renin release was studied in rat kidney slices ia vitro. Renin release was an active, linear, and temperature-dependent process. Kidneys from youag rats released ranch more renin thaa those from adults. Lowering sodium concentration inhibited renin release by one-half, even when osmolality was kept constant. Isoproterenol (10 ' to 10 ' M) stimulated reain release signifkaatJy in a partially dose-related manner, ^/-propranolol inhibited this stinralatioa. Significant (P < 0.05) inhibition of reain release was induced by /-epiaephrine or /-•oreplaephrine (10'° M). la the presence of aa a-receptor Mocking drag, pbeBoxybeazamine (10" • M), inhibition no longer occurred with epinephrine and stimulation was observed with /-aorepinephrine. Vutcristine (10** M) did not affect renin release when slices from the kidneys of normal rats or adrenalectomlzed, sodium-depleted rats were incubated, but significantly inhibited (P < 0.01) release that had been stimulated in vitro by isoproterenol. These results suggest to us that there may be (1) a direct or indirect (aiediated through the macula densa) effect of sodium on juxtaglomerular cells (2) an inhibitory role for a-adrenergic receptors on renin release, in addition to the stimulatory role of /3-receptors, (3) possible participation of microtubules in isoproterenol-stimulated renin release, and (4) an alternative mode of secretion of renin under stimulation by adrenalectomy and salt depletion.INCUBATION of kidney slices in vitro is a practical approach for studying the variables which affect renin secretion. However, even in this simple system, some points remain unsettled. For example, it is not clear whether sodium concentration of the incubation medium plays an important role, or whether changes in renin release observed after modifying sodium concentration actually depend only on changes in osmolality.1 " 8 Furthermore, although studies in vivo generally point to a stimulatory role of 0-adrenergic receptors on renin release,* there has been some controversy concerning the effect of catecholamines on renin release in vitro.7 "" Also, the role, if any, of a-adrenergic receptors still is to be elucidated. Finally, the mechanisms by which renin is released remain unknown. Since secretory granules containing renin have been shown in the epithelioid cells of the juxtaglomerular apparatus, 11 "" it is possible that secretion is mediated, as in other polypeptide-secreting cells, by the microtubular-microfilamentous system."Our present study was undertaken to clarify the effect of sodium in the incubation medium on renin release by rat kidney slices, as well as the role of a-and /8-adrenergic agonists and inhibitors. Furthermore, we tested the hypothesis that renin release is dependent on the microtubular system by studying the effect of a microtubular inhibitor, vincristine, on renin secretion. Methods INCUBATION OF KIDNEY SLICESMale Wistar rats (300-350 g...

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Section: Table 4 Effect Of Propranolol On Renin Release In Vitro Stimmentioning

confidence: 54%

Section: Incubation Of Kidney Slicesmentioning

confidence: 99%

Renin release by rat kidney slices incubated in vitro. Role of sodium and of alpha- and beta-adrenergic receptors, and effect of vincristine.

Capponi¹,

Vallotton²

1976

Circulation Research

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The effect of naringenin on the intestinal and renal transport of organic solutes

Robinson

L'Herminier

Claudet

1979

Naunyn-Schmiedeberg's Arch. Pharmacol.

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Active amino-acid and sugar uptake by gall bladder epithelium in dog, guinea-pig and man

et al. 1975

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Slices of dog gall bladder are capable of accumulating amino-acids and sugars against considerable concentration gradients across the luminal membrane of the cell. The epithelium of the common bile duct also absorbs these substrates. The transport systems are sodium-dependent, saturable and inhibited by ouabain and metabolic poisons. The specificity of the mechanisms is more reminiscent of kidney than of intestinal transport. Glycine is preferentially transported across dog gall bladder from the mucosa to the serosa, but the net flux is small. It is concluded that the contraluminal membrane of the epithelial cell might be relatively impermeable to the amino-acid. concentrative uptake also occurs in guinea-pig gall bladder; however it appears to be practically non-existent in rabbit tissue. Human gall bladders, obtained at random from the operating room, also displayed active accumulation. The gall bladder epithelium is remarkably resistant to anoxia. Furthermore, following obstruction of the common bile duct for 2 weeks in dogs, the gall bladder is still able to concentrate amino-acids and sugars, but the extent of the uptake is significantly reduced.

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V�rification de l'efficacit� d'une guillotine multilames pour la pr�paration standardis�e de tranches tissulaires

Cited by 12 publications

References 4 publications

Renin release by rat kidney slices incubated in vitro. Role of sodium and of alpha- and beta-adrenergic receptors, and effect of vincristine.

Renin release by rat kidney slices incubated in vitro. Role of sodium and of alpha- and beta-adrenergic receptors, and effect of vincristine.

The effect of naringenin on the intestinal and renal transport of organic solutes

Active amino-acid and sugar uptake by gall bladder epithelium in dog, guinea-pig and man

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