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Veiko, Natalia N.; Lyapunova, N. A.; Kosyakova, Nadezda; Spitkovskii, D. M.

doi:10.1023/a:1004898617333

Cited by 8 publications

(3 citation statements)

References 15 publications

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“…The different rate of hydrolysis can be explained by the existence of protein complexes protecting some DNA sequences from nucleases or by intrinsic properties of these sequences. We previously showed that TRrDNA is more resistant to double-strand breaks due to accumulation of single-strand breaks (resulting from nuclease hydrolysis or chemical modification) than other sequences (satellite 3 subfragment, Alu-repeat, or histone genes) [2]. A possible mechanism of this phenomenon is elevated melting temperature of GC-rich rDNA with single-strand breaks.…”

Section: Resultsmentioning

confidence: 99%

“…DNA sequences containing unmethylated CpG-motifs interact with Tolllike receptors (TLR9) in the cells and up-regulate synthesis of cytokines via stimulation of B-lymphocytes and plasmacytoid dendritic cells [2,8,14,15]. Therefore, unmethylated DNA-CpG motifs strengthen immunity during preventive and therapeutic vaccination [9].…”

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confidence: 99%

“…In the organism, extracellular host DNA can differ significantly from nuclear DNA by the content of unmethylated CpG motifs. We previously hypothesized that various fragments of genomic DNA in humans can differently accumulate in the serum and plasma of the peripheral blood [2]. It was demonstrated that GC-enriched transcribed region of ribosomal repeat (TRrDNA) is resistant to the effect of double-strand breaks due to accumulation of single-strand breaks under conditions of nuclease hydrolysis, and can accumulate in the blood.…”

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confidence: 99%

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Blood serum DNA in patients with rheumatoid arthritis is considerably enriched with fragments of ribosomal repeats containing immunostimulatory CpG-motifs

et al. 2006

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We previously hypothesized that the sequence of transcribed region of human ribosomal repeats is selectively accumulated in circulating extracellular DNA due to its increased resistance to double-strand breaks caused by accumulation of single-chain breaks produced by nucleases. The contents of rDNA in blood serum DNA and in DNA from leukocytic nuclei both in healthy donors and in patients with rheumatoid arthritis were compared using dot hybridization method. By the content of non-methylated CpG-repeats, transcribed region of rDNA is identical to bacterial DNA, which is characterized by potent immunostimulatory effect. The transcribed region of rDNA (13.3 kb) contains more than 200 CpG-motifs capable of interacting with TLR9 receptors, which are the mediators of the cell immune response to the action of CpG-rich DNA fragments. The data suggest that DNA from dead cells circulating in the peripheral blood is enriched with sequences possessing potent immunostimulatory properties.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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