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Abstract

During remodeling progress post myocardial infarction, the contribution of neoangiogenesis to the infarct-bed capillary is insufficient to support the greater demands of the hypertrophied but viable myocardium resulting in further ischemic injury to the viable cardiomyocytes at risk. Here we reported the bio-assay-guided identification and isolation of angiogenic tannins (angio-T) from Geum japonicum that induced rapid revascularization of infarcted myocardium and promoted survival potential of the viable cardiomyocytes at risk after myocardial infarction. Our results demonstrated that angio-T displayed potent dual effects on up-regulating expression of angiogenic factors, which would contribute to the early revascularization and protection of the cardiomyocytes against further ischemic injury, and inducing antiapoptotic protein expression, which inhibited apoptotic death of cardiomyocytes in the infarcted hearts and limited infarct size. Echocardiographic studies demonstrated that angio-T-induced therapeutic effects on acute infarcted myocardium were accompanied by significant functional improvement by 2 days after infarction. This improvement was sustained for 14 days. These therapeutic properties of angio-T to induce early reconstitution of a blood supply network, prevent apoptotic death of cardiomyocytes at risk, and improve heart function post infarction appear entirely novel and may provide a new dimension for therapeutic angiogenesis medicine for the treatment of ischemic heart diseases.

The Antiapoptosis Effect of Geum japonicum Thunb. var. chinense Extracts on Cerebral Ischemia Reperfusion Injury via PI3K/Akt Pathway

“…The antiapoptosis effect may cause the restoration of blood supply or the initiation of the intracerebral revascularization. The effect of GJ extracts was also observed in the ischemic myocardium by Gu et al [ 47 ], who in their study has demonstrated that GJ extracts could promote the expression of angiogenic factors, restore early revascularization, and improve blood supply to ischemic myocardium. At the same time, it could also induce the expression of antiapoptotic protein Bcl-2 in infarcted hearts and inhibit the apoptosis of cardiomyocytes.…”

Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells

“…Perfusion technique has been used to isolate endothelial cells of the heart especially from the coronary artery endothelial cells [ 6 – 11 ]. Magnetic bead cell sorting using single [ 12 ] or multiple markers [ 13 – 16 ] has been performed to purify endothelial cells from the heart. Flow cytometry has been used to sort cells after labeling with DiI-Ac-LDL [ 17 , 18 ].…”