Vaccination against Taenia solium cysticercosis in underfed rustic pigs of México: roles of age, genetic background and antibody response

Huerta, Mario; Sciutto, Edda; Garcia, G.; Villalobos, Nelly; Hernández, Marisela; Fragoso, Gladis; Díaz, J. C.; Diaz, Ana María; Ramirez, Ruben D.; Luna, Susana de la; García, José; Aguilar, Edenil Costa; Espinoza, Surizaray G; Castilla, G; Bobadilla, Juan R.; Ávila, Ricardo Andrez Machado de; José, Marco V.; Larralde, Carlos; Aluja, Aline S. de

doi:10.1016/s0304-4017(00)00233-8

Cited by 23 publications

(11 citation statements)

References 17 publications

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“…Curiously, such infection leads to the increased feminization of both male and female mice, with increases in oestradiol in all the mice and a decrease in testosterone in the male mice (Larralde et al, 1995). With T. solium, castration and pregnancy have been found to increase the prevalence of cysticercosis in pigs, presumably through changes in the concentrations of some sex hormones (Morales et al, 2002), and histological damage to T. solium cysts in pigs vaccinated against the parasite was found to be greater in the older hosts than in the younger animals (Huerta et al, 2000). Although age (Fleury et al, 2004;Sáenz et al, 2006) and gender Fleury et al, 2004) differences in immune response to T. solium infection of the human CNS have also been reported, concern has been raised about the possibility that selection bias is responsible for these apparent differences (Thurn, 1988).…”

Section: Discussionmentioning

confidence: 99%

The association of host age and gender with inflammation around neurocysticercosis cysts

Kelvin¹,

Carpio²,

Bagiella³

et al. 2009

Annals of Tropical Medicine & Parasitology

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The results of previous investigations indicate that age and gender may influence the strength of the human host's immune response to infection of the central nervous system with the larvae of Taenia solium. Most of the relevant research on such neurocysticercosis (NCC) has, however, been conducted on hospital-based samples in developing countries, where differential access to healthcare may bias the study results. Using data from 171 NCC patients participating in a treatment trial, the associations of patient age and gender with the presence of inflammation around NCC cysts (i.e. cysts in the transitional phase) have recently been explored, after controlling for measures of economic and geographical access to healthcare. Data on cysts were collected from computed-tomography or magnetic-resonance images taken at four time-points, from baseline to 12-months post-treatment. The odds of having transitional cysts were evaluated by logistic regression whereas Poisson regression was used to explore the numbers of transitional cysts, with generalised estimating equations (GEE) used to account for the multiple observations over time.After controlling for healthcare access, the odds of having transitional cysts were found to be 1.5-fold higher for the female patients than for the male, although this association was not statistically significant (P50.136). In the Poisson model, however, the number of transitional cysts was found to be 1.8-fold higher in the female patients than in the male, and this gender effect was not only statistically significant (P50.002) but also constant over time. The association of host age with transitional cysts was more complicated, with significant interaction between age and time. It therefore appears that there are significant gender and age differences in the local immune response to NCC, even after adjusting for differences in healthcare access.

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Section: Discussionmentioning

confidence: 99%

The association of host age and gender with inflammation around neurocysticercosis cysts

Kelvin¹,

Carpio²,

Bagiella³

et al. 2009

Annals of Tropical Medicine & Parasitology

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“…Cells from mice injected with saponin (controls) showed no proliferative response above background levels. Figure 4 shows that stimulated cells increased from 3.5 or 4.5% to [8][9][10][11][12][13][14][15][16].3% when the cells were primed with TcAg or the appropriate peptide, respectively. Stimulated cells were enriched in both CD4 ϩ and CD8 ϩ cells by factors of 1.2 to 2.0 for CD4 ϩ and 3.9 to 4.9 for CD8 ϩ .…”

Section: Resultsmentioning

confidence: 99%

“…However, since the pig is an indispensable intermediate host, transmission could be hindered by lowering the prevalence of pig cysticercosis through vaccination. Development of an effective vaccine to be used in pigs is being pursued by a number of scientists, with promising results (9,(15)(16)(17).…”

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confidence: 99%

Two Epitopes Shared by Taenia crassiceps and Taenia solium Confer Protection against Murine T. crassiceps Cysticercosis along with a Prominent T1 Response

et al. 2001

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“…Information on vaccination against cysticercosis using different antigens is growing rapidly and has been recently reviewed (6,15,16,27). Previous reports suggested that protection against murine cysticercosis by T. crassiceps is mediated by a Th1 response (32,34) featured by CD4 ϩ or CD8 ϩ T cells, implying that T lymphocytes can induce protection irrespective of their phenotypes (17,31,33).…”

Section: Discussionmentioning

confidence: 99%

Characterization and Protective Potential of the Immune Response toTaenia soliumParamyosin in a Murine Model of Cysticercosis

et al. 2001

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Paramyosin has been proposed as a vaccine candidate in schistosomiasis and filariasis. However, limited information is available about its protective potential against cysticercosis and the immune response it induces. Immunization of mice with recombinant full-length paramyosin of Taenia solium (TPmy) results in about a 52% reduction in parasite burden after a subsequent challenge by intraperitoneal inoculation of Taenia crassiceps cysticerci. Immunization assays using recombinant fragments of TPmy, corresponding approximately to thirds on the amino, central, or carboxyl regions, suggest that protective epitopes are located mostly in the amino-end third. Proliferation assays using T cells obtained from mice immunized with the full-length recombinant TPmy also showed a preferential response to the amino-terminal fragment. In contrast, antibodies in the sera from these mice predominantly recognize epitopes located in the carboxyl-terminal fragment, being the immunoglobulin G1 subclass, the predominant antibody isotype. Characterization of the cellular immune response induced against the protective amino-terminal fragment reveals production of gamma interferon and interleukin-2, but not interleukin-4, suggesting a Th1-like profile.Paramyosin (Pmy) is a filamentous, ␣-helical, coiled-coil protein of about 100 kDa, present in some muscles of invertebrates. It is also an antigen during infections by several flatworms that are important parasites of humans and of domestic animals such as Schistosoma mansoni (10), Schistosoma japonicum (4), Taenia solium (10, 12), and Echinococcus granulosus (18). The paramyosin of T. solium (TPmy) is present in the musculature but has also been found associated with the tegument of the parasite (7). The collagen-binding and complement-inhibitory properties of TPmy have been described previously (8, 9, 11). TPmy is synthesized by the tegumentary cytons and apparently released through the cyst tegument (8). Furthermore, TPmy can be collected in the culture medium in which T. solium cysts are maintained (8), suggesting that a similar release to the host tissues might occur in vivo and that TPmy may modulate the host response through diminution of the inflammatory mediators at the host-parasite interface (8,11).Paramyosins have been proposed as vaccine candidates in a number of helminthiases including schistosomiasis (3, 20) and filariasis (14,19). Despite their protective abilities against schistosomiasis and filariasis, limited information is available on their potential as vaccines against cysticercosis. Here we report that immunization of mice with recombinant fragments of TPmy induces significant levels of protection in the murine model of cysticercosis by Taenia crassiceps. The profile of cytokine production suggests that the protective amino-terminal fragment of TPmy induces a Th1-like immune response. MATERIALS AND METHODSAnimal model. Mice used in all experiments were 4-to 6-week-old female BALB/c AnN strain mice. The ORF strain of T. crassiceps was maintained by consecutive passa...

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Vaccination against Taenia solium cysticercosis in underfed rustic pigs of México: roles of age, genetic background and antibody response

Cited by 23 publications

References 17 publications

The association of host age and gender with inflammation around neurocysticercosis cysts

The association of host age and gender with inflammation around neurocysticercosis cysts

Two Epitopes Shared by Taenia crassiceps and Taenia solium Confer Protection against Murine T. crassiceps Cysticercosis along with a Prominent T1 Response

Characterization and Protective Potential of the Immune Response toTaenia soliumParamyosin in a Murine Model of Cysticercosis

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