Vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in its fry against Aeromonas hydrophila

Abstract

The aim of this study was to analyze the effectivity of vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in fry tilapia against Aeromonas hydrophila. Tilapia Nirwana strain that used for this had average body weight of 185±13.23 g and were maintained in ponds sizing of (2.5×2.5×1 m 3 ). Vaccinations that has been done through intraperitoneal injection using dose of 0.1 mL/fish, meanwhile the fish for control was injected by phosphate buffered saline (PBS). This study used complete randomized design with two treatments and three replications. Antibody level was measured by using indirect enzyme-linked immunosorbent assay (ELISA) method in the broodstock, egg, and fry. Challenge test in fry tilapia performed at the age of 5, 10, and 15 days. The results showed that vaccination in tilapia broodstock delivered a significant antibody level in broodstock, eggs, and fry (P<0.05) compared to the control. Relative percent survival of offspring at 5, 10, and 15 days were 78.26%, 70.59%, and 65.52%, respectively. As a conclusion, vaccination in tilapia broodstock was effective to improve specific and non-specific immunity, and protect fry tilapia from A. hydrophila infection through maternal immunity.Keywords: vaccination, antibody, maternal immunity, tilapia, Aeromonas hydrophila ABSTRAK Penelitian ini bertujuan untuk menganalisis efikasi vaksinasi pada induk nila dengan vaksin sel utuh dan ketahanan benih yang dihasilkan terhadap Aeromonas hydrophila. Ikan nila stain Nirwana yang digunakan dalam penelitian memiliki bobot rata-rata 185±13,23 g dan ikan dipelihara dalam kolam (2,5×2,5×1 m 3 ). vaksinasi dilakukan melalui penyuntikan intraperitoneal dengan dosis 0,1 mL/ikan, sementara itu ikan kontrol disuntik dengan phosphate buffered saline (PBS). Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Tingkat antibodi diukur dengan menggunakan metode indirect enzyme-linked immunosorbent assay (ELISA) pada induk, telur dan benih. Uji tantang pada benih dilakukan pada umur 5, 10, dan 15 hari. Hasil penelitian menunjukan bahwa vaksinasi pada induk nila secara signifikan dapat meningkatkan level antibodi pada induk, telur, dan benih (P<0,05) dibandingkan dengan kontrol. Kelangsungan hidup relatif pada benih berumur 5, 10, dan 15 hari masing-masing adalah 78,26%; 70,59%; dan 65,52%. Sebagai kesimpulan vaksinasi pada induk nila efektif dalam memperbaiki imunitas spesifik dan non spesifik serta melindungi benih dari infeksi A. hydrophila melalui imunitas maternal.

Maternal immunity of tilapia broodstock vaccinated with polyvalent vaccine and resistance of their offspring againstStreptococcus agalactiae

“…Indicators determining the health status of fish in the form of haematological parameter can be obtained by observing blood properties (Abu‐Elala, Samir, Wasfy, & Elsayed, ; Silva et al, ; Sukenda, Carman, Rahman, Hidayatullah, & Yumaidawati, ; Sukenda, Rahman, et al, ). The observations of blood properties (Table ) showed that the total leukocytes, phagocytic activity and respiratory burst experienced a significant increase between vaccinated broodstock using polyvalent vaccine compared with unvaccinated broodstock.…”

“…The observations of blood properties (Table ) showed that the total leukocytes, phagocytic activity and respiratory burst experienced a significant increase between vaccinated broodstock using polyvalent vaccine compared with unvaccinated broodstock. Total leukocytes in vaccinated broodstock experienced a significant increase compared with unvaccinated broodstock (Abu‐Elala et al, ; Sukenda et al, ). The body's response to recognize antigen was marked by increased leukocytes which can stimulate the formation of antibody (Hardi, Harris, & Lusiastuti, ; Purwanti & Sudaryono, ).…”

“…Lysozyme activity in the broodstock, eggs and larvae from the spawning of vaccinated broodstock was higher than that of the treatment without unvaccinated (Sukenda, Rahman, et al, ). Previous research also mentioned that lysozyme and antibody from vaccinated broodstock can be transferred and can provide protection to the produced larvae (Mingming, Fu, Zhen, & Jilin, ; Sukenda et al, ). Lysozyme transferred by the broodstock to the eggs serves to prevent vertical transmission of disease from several types of pathogens (Zhang et al, ).…”

“…The RPS of tilapia larvae after the challenge test was shown in Sukenda et al, 2017). The body's response to recognize antigen was marked by increased leukocytes which can stimulate the formation of antibody (Hardi, Harris, & Lusiastuti, 2013;Purwanti & Sudaryono, 2014).…”

Haemato-immunological responses and effectiveness of feed-based bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila infections in hybrid red tilapia (Oreochromis mossambicus × O. niloticus)

“…In this study, the haemoglobin and haematocrit percentage in vaccinated sh did not show a signi cant different compared to the unvaccinated sh after immunization. Sukenda [43] also documented that the haemoglobin and haematocrit percentage of vaccinated tilapia were not signi cantly different from unimmunized sh. Once again, both haemoglobin and haematocrit level of immunized tilapia was increased following infection with S. iniae and A. hydrophila, which was also observed in immunized Nile tilapia after infection with A. hydrophila [44].…”