Vaccination of Cats with Attenuated Feline Immunodeficiency Virus Proviral DNA Vaccine Expressing Gamma Interferon

Gupta, Surabhi; Leutenegger, Christian M.; Dean, Gregg A.; Steckbeck, Jonathan D.; Cole, Kelly Stefano; Sparger, Ellen E.

doi:10.1128/jvi.00815-06

Cited by 17 publications

(27 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Humoral immunity was assessed at the start of immunization, when all animals were uniformly negative (data not shown), at boost, and at challenge. In line with data from previously reported FIV DNA vaccination studies (24,25,37,54), at boost, serology was still essentially negative except for two vaccinees (cats GD and GE) who, somewhat surprisingly, showed measurable anti-Env binding antibodies and NA. On the other hand, at challenge, all vaccinees possessed moderate to high levels of anti-whole-FIV antibodies and anti-Env binding antibodies, and, most impor- (Table 1).…”

Section: Resultssupporting

confidence: 75%

Env-Expressing Autologous T Lymphocytes Induce Neutralizing Antibody and Afford Marked Protection against Feline Immunodeficiency Virus

Pistello

Bonci

Zabogli

et al. 2010

J Virol

View full text Add to dashboard Cite

The envelope (Env) glycoproteins of HIV and other lentiviruses possess neutralization and other protective epitopes, yet all attempts to induce protective immunity using Env as the only immunogen have either failed or afforded minimal levels of protection. In a novel prime-boost approach, specific-pathogen-free cats were primed with a plasmid expressing Env of feline immunodeficiency virus (FIV) and feline granulocyte-macrophage colony-stimulating factor and then boosted with their own T lymphocytes transduced ex vivo to produce the same Env and interleukin 15 (3 ؋ 10 6 to 10 ؋ 10 6 viable cells/cat). After the boost, the vaccinees developed elevated immune responses, including virus-neutralizing antibodies (NA). Challenge with an ex vivo preparation of FIV readily infected all eight control cats (four mock vaccinated and four naïve) and produced a marked decline in the proportion of peripheral CD4 T cells. In contrast, five of seven vaccinees showed little or no traces of infection, and the remaining two had reduced viral loads and underwent no changes in proportions of CD4 T cells. Interestingly, the viral loads of the vaccinees were inversely correlated to the titers of NA. The findings support the concept that Env is a valuable immunogen but needs to be administered in a way that permits the expression of its full protective potential.

show abstract

Section: Resultssupporting

confidence: 75%

Env-Expressing Autologous T Lymphocytes Induce Neutralizing Antibody and Afford Marked Protection against Feline Immunodeficiency Virus

Pistello

Bonci

Zabogli

et al. 2010

J Virol

View full text Add to dashboard Cite

show abstract

“…In cases of viral infections, there is a general presumption that Th1 responses are safe and antiviral but that Th2 responses are pathogenic, but the present results instead show the opposite. While cytokines can be used as effective adjuvants in some models (12), their use needs to be carefully considered on a case-by-case basis.…”

Section: Discussionmentioning

confidence: 99%

Virally Delivered Cytokines Alter the Immune Response to Future Lung Infections

Harker

Bukreyev

Collins

et al. 2007

J Virol

View full text Add to dashboard Cite

Respiratory syncytial virus (RSV) is an important cause of infant morbidity and mortality worldwide and is increasingly recognized to have a role in the development and exacerbation of chronic lung diseases. There is no effective vaccine, and we reasoned that it might be possible to skew the immune system towards beneficial nonpathogenic responses by selectively priming protective T-cell subsets. We therefore tested recombinant RSV ( ؉ T cells were observed after challenge in mice primed with rRSV/IFN-␥. By contrast, rRSV/IL-4-primed mice were protected against weight loss during secondary challenge but showed airway eosinophilia. When rRSV/IL-4-primed mice were challenged with influenza virus, weight loss was attenuated but was again accompanied by marked airway eosinophilia. Thus, immunization directed toward enhancement of Th1 responses reduces viral load but is not necessarily protective against disease. Counter to expectation, Th2-biased responses were more beneficial but also influenced the pathological effects of heterologous viral challenge.

show abstract

“…Virus-specific T cell proliferative responses were tested in PBMC prepared from vaccinated and control cats as previously described [16]. Briefly, PBMC were isolated by ficoll-hypaque separation and plated at 10 6 cells per well in a 24 well plate in standard PBMC media without IL-2.…”

Section: Methodsmentioning

confidence: 99%

“…Data from 60,000 events for each sample were collected and analyzed. A significant proliferation response was defined as equal to, or greater than, 2% FIV-specific proliferating T cells, based on values previously established for uninfected control cats [16]. …”

Section: Methodsmentioning

confidence: 99%

“…One vaccine study revealed that immunization of cats with FIV-pPPRΔvif proviral DNA alone elicited protection against challenge with homologous wild type FIV-PPR [15]. Later studies investigating this FIV-pPPRΔvif DNA vaccine revealed improved antigen-specific cellular immune responses by co-expression of feline IFN-γ from the vif -deleted FIV provirus [16]. However, the latter study revealed poor efficacy for the FIV-pPPRΔvif DNA vaccine, with or without incorporation of IFN-γ co-expression.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Vaccination with vif-deleted feline immunodeficiency virus provirus, GM-CSF, and TNF-α plasmids preserves global CD4 T lymphocyte function after challenge with FIV

Maksaereekul

Dubie

Shen

et al. 2009

Vaccine

Self Cite

View full text Add to dashboard Cite

Feline immunodeficiency virus (FIV) DNA vaccine approaches that included a vif-deleted FIV provirus (FIV-pPPRΔvif) and feline cytokine expression plasmids were tested for immunogenicity and efficacy by immunization of specific pathogen free cats. Vaccine protocols included FIV-pPPRΔvif plasmid alone; a combination of FIV-pPPRΔvif DNA and feline granulocyte macrophage-colony stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-α expression plasmids; or a combination of FIV-pPPRΔvif and feline interleukin (IL)-15 plasmids. Cats immunized with FIV-pPPRΔvif, GM-CSF and TNF-α plasmids demonstrated an increased frequency of FIV-specific T cell proliferation responses compared to other vaccine groups. Immunization with FIV-pPPRΔvif and IL-15 plasmids was distinguished from other vaccine protocols by the induction of antiviral antibodies. Suppression of virus loads was not observed for any of the FIV-pPPRΔvif DNA vaccine protocols after challenge with the FIV-PPR isolate. However, prior immunization with FIV-pPPRΔvif, GM-CSF, and TNF-α plasmids resulted in preservation of CD4 T cell functions, including mitogen-induced cytokine expression and antigen-specific proliferation upon infection with FIV. These findings justify further examination of cytokine combinations as adjuvants for lentiviral DNA vaccines.

show abstract

Vaccination of Cats with Attenuated Feline Immunodeficiency Virus Proviral DNA Vaccine Expressing Gamma Interferon

Cited by 17 publications

References 37 publications

Env-Expressing Autologous T Lymphocytes Induce Neutralizing Antibody and Afford Marked Protection against Feline Immunodeficiency Virus

Env-Expressing Autologous T Lymphocytes Induce Neutralizing Antibody and Afford Marked Protection against Feline Immunodeficiency Virus

Virally Delivered Cytokines Alter the Immune Response to Future Lung Infections

Vaccination with vif-deleted feline immunodeficiency virus provirus, GM-CSF, and TNF-α plasmids preserves global CD4 T lymphocyte function after challenge with FIV

Contact Info

Product

Resources

About