Vaccination of cattle with a DNA plasmid encoding the bovine viral diarrhoea virus major glycoprotein E2

Harpin, Serge; Hurley, David J.; Mbikay, Majambu; Talbot, Brian G.; Elazhary, Youssef

doi:10.1099/0022-1317-80-12-3137

Cited by 48 publications

(26 citation statements)

References 28 publications

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“…However, there is concern that neither of these traditional types of vaccine is optimal for controlling BVDV infection (48). For that reason, the possibility of using immunodominant proteins of BVDV in the form of DNA (23,24,(35)(36)(37) or protein subunit (6,9,10) vaccines has gained widespread interest. The use of protein and DNA vaccines provides an opportunity to introduce vaccines that are arguably the safest vaccines that can be developed.…”

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confidence: 99%

Fusion to C3d Enhances the Immunogenicity of the E2 Glycoprotein of Type 2 Bovine Viral Diarrhea Virus

Wang

Sunyer

Bello

2004

J Virol

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The use of DNA and protein subunit vaccines in animals provides an opportunity to introduce vaccines that are arguably the safest that can be developed. For that reason, considerable effort is under way to devise methods of enhancing the immunogenicity of such vaccines. Seven years ago it was shown that fusing complement fragment C3d to hen egg lysozyme (HEL) enhanced the immunogenicity of HEL 10,000-fold. Based on this observation, we decided to evaluate the effect of C3d on the immunogenicity of the E2 protein of bovine viral diarrhea virus (BVDV). E2 is the major target of neutralizing antibody during BVDV infection. To test the effect of C3d on E2 immunogenicity, expression cassettes encoding a secreted form of E2 alone (E2s) or E2 fused to three copies of murine C3d (E2s-C3d) were constructed. The proteins were purified from the supernatants of transfected cells and used to immunize mice. The immune response was monitored by an enzyme-linked immunosorbent assay (ELISA) for E2s-specific antibody and by a virus neutralization test. The ELISA results indicated that the E2s-C3d protein is 10,000-fold more immunogenic than the E2s protein alone. The maximum primary immune response was elicited with <0.1 g of E2s-C3d protein without an adjuvant. In addition, we have shown for the first time that high levels of anti-E2s and neutralizing antibodies can be elicited when this same low concentration of E2s-C3d is used to both prime and boost the immune response. We conclude that the E2s-C3d fusion protein has significant potential as a subunit vaccine against BVDV infection.

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confidence: 99%

Fusion to C3d Enhances the Immunogenicity of the E2 Glycoprotein of Type 2 Bovine Viral Diarrhea Virus

Wang

Sunyer

Bello

2004

J Virol

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“…In recent years, several research teams have reported DNA immunisation to BVDV structural proteins in the mouse [26,42,45,46] and cattle [27,47,42]. All experiments involved E2 constructs from a single BVDV-1a viral strain.…”

Section: Discussionmentioning

confidence: 99%

“…In most reports, cross-neutralisation with strains from other genotypes was not tested. Harpin et al [26,27] showed that 3 / 4 vaccinated mice cross-neutralised BVDV-1b strain New-York as well as BVDV-1a NADL but not BVDV-2 strain 125, indicating that cross-neutralisation is restricted to the genotype from which the coding sequence had been cloned. Another report by Nobiron et al [46] showed that the response to heterologous strain NADL was much lower in terms of titres and numbers of responding mice than to homologous strain Ky1203nc though both belong to the same genotype Ia.…”

Section: Discussionmentioning

confidence: 99%

Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

et al. 2007

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-We report DNA immunisation experiments in cattle using plasmid constructs that encoded glycoprotein E2 from bovine viral diarrhoea virus (BVDV)-1 (E2.1) and BVDV-2 (E2.2). The coding sequences were optimised for efficient expression in mammalian cells. A modified leader peptide sequence from protein gD of BoHV1 was inserted upstream of the E2 coding sequences for efficient membrane export of the proteins. Recombinant E2 were efficiently expressed in COS7 cells and they presented the native viral epitopes as judged by differential recognition by antisera from cattle infected with BVDV-1 or BVDV-2. Inoculation of pooled plasmid DNA in young cattle elicited antibodies capable of neutralising viral strains representing the major circulating BVDV genotypes.BVDV / DNA immunisation / E2 / neutralisation / synthetic gene

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“…A BVDV-la DNA vaccine induced neutralising antibody response and cell-mediated immune response in cattle but gave a limited protection against a heterologous BVDV-lb strain (36).…”

Section: Protective Efficacy Of Current Bvdv Vaccinesmentioning

confidence: 99%

Bovine viral diarrhoea virus (BVDV) diversity and vaccination. A review

Kalaycioglu¹

2007

Veterinary Quarterly

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Vaccination of cattle with a DNA plasmid encoding the bovine viral diarrhoea virus major glycoprotein E2

Abstract: Bovine viral diarrhoea virus (BVDV) is an economically important pathogen of cattle that is

Cited by 48 publications

References 28 publications

Fusion to C3d Enhances the Immunogenicity of the E2 Glycoprotein of Type 2 Bovine Viral Diarrhea Virus

Fusion to C3d Enhances the Immunogenicity of the E2 Glycoprotein of Type 2 Bovine Viral Diarrhea Virus

Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

Bovine viral diarrhoea virus (BVDV) diversity and vaccination. A review

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