Vaccination with virus-like particles of atypical porcine pestivirus inhibits virus replication in tissues of BALB/c mice

Abstract

Congenital tremor (CT) type A-II in piglets is a worldwide disease caused by an emerging atypical porcine pestivirus (APPV). Preparation and evaluation of vaccines in laboratory animals is an important preliminary step toward prevention and control of the disease. Here, virus-like particles (VLPs) of APPV were prepared and VLPs vaccine was evaluated in BALB/c mice. Purified E rns and E2 proteins expressed in E. coli were allowed to self-assemble into VLPs, which had the appearance of hollow spherical particles with a diameter of about 100 nm by transmission electron microscopy (TEM). The VLPs induced strong antibody responses and reduced the viral load in tissues of BALB/c mice. The data from animal challenge experiments, RT-PCR, and immunohistochemical analysis demonstrated that BALB/c mice are an appropriate laboratory model for APPV. These results suggest the feasibility of using VLPs as a vaccine for the prevention and control of APPV and provide useful information for further study of APPV in laboratory animals.

Heterologous expression of Chimeric hepatitis B core virus like particles harboring SARS-CoV2 epitope and evaluation of its immunization potential in mice

“…Although Freund's adjuvant might not be safe for humans, because it is reported to be more highly immunogenic than other adjuvants in mice [41,42], we preferably used this adjuvant to examine the general response of HBc-E VLP in the presence to a potent adjuvant in comparison with immunized mice that were solely injected by HBc-E VLP. Similarly, this adjuvant has been used in different reports due to its effectiveness in antibody production [43,44,45,46]. Moreover, as it was expected for the SARS-CoV-2 vaccine, in both with and without adjuvant conditions, the IgG titer was normally higher four weeks after injection than two weeks later.…”

Senecavirus A-induced glycolysis facilitates virus replication by promoting lactate production that attenuates the interaction between MAVS and RIG-I

“…qRT-PCR was performed using the SYBR Premix Ex Taq Kit (Takara, Japan) with specific primers ( S1 Table ) in a Light Cycler 480 (Roche, Switzerland). The relative expressions of the target genes were calculated using the 2 −ΔΔCt method, as described previously [ 47 ].…”

ATYPICAL PORCINE PESTIVIRUS (Pestivirus K) — A NEW CHALLENGE FOR PIG FARMING (review)

“…В настоящее время вакцины для профилактики инфекции, вызванной АПС у свиней, не разработаны. Сообщается о создании двух вакцин и их эффективности для предотвращения инфекции у мышей линии BALB/c (77,79). Как и при других вирусных инфекциях, важно не допустить заноса возбудителя в стадо.…”