Vacuolar status and water relations in embryonic axes of recalcitrant Aesculus hippocastanum seeds during stratification and early germination

Obroucheva, N. V.; Lityagina, S. V.; Новикова, Г. В.; Sinkevich, I.A.

doi:10.1093/aobpla/pls008

Cited by 22 publications

(27 citation statements)

References 38 publications

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“…Also in the forest species Aesculus hippocastanum aquaporin gene expression is low during the beginning of imbibition and only at radicle emergence it is strongly activated. The authors suggest the presence of aquaporins in the seeds during imbibition phase, but in a closed state (Obroucheva et al 2012;Obroucheva 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Although the use of mercury can not by itself provide definitive evidence for aquaporins function, it allows us to indicate possible activity and function of these proteins (Willigen et al 2006) and its use is still found in current works (Obroucheva et al 2012;Liu et al 2014). Novikova et al (2014) say that in growing organs, and in the absence of suitable genetically altered materials, studies performed with mercury or other pharmacological blockers remain the only accessible technique for probing the functioning of aquaporins.…”

Section: Discussionmentioning

confidence: 99%

“…Reducing agents, including dithiothreitol (DTT) and β-mercaptoethanol, have also been used in conjunction with HgCl 2 in current studies that seek to evaluate the activity of aquaporins (Przedpelska-Wasowicz & Wierzbicka 2011;Obroucheva et al 2012;Liu et al 2014). They reduce the sulfhydryl groups that are oxidized by mercury, allowing these proteins to reassume their original conformation and functionality.…”

Section: Introductionmentioning

confidence: 99%

“…Among them, one can cite works with rice (Liu et al 2013), tomato (Jain et al 2008), broad bean (Novikova et al 2014), and pea (Veselova & Veselovsky 2006). However, only one data on forest species is currently available (Obroucheva et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins

et al. 2015

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Studies that evaluate the physiological and biochemical mechanisms of germination within forest species are needed in order to improve our understanding of such processes. Mercury and dithiothreitol are indicated as important tools in studies that assess the activity of aquaporins during imbibition and germination of seeds. To investigate the alterations caused by mercury in Plathymenia reticulata seeds, different doses of mercury were used in the presence and absence of dithiothreitol. Mercury had a dose-dependent effect on the seeds; in the most dilute solutions mercury partially inhibited the imbibition process, whereas in the most concentrated solutions it caused the death of the embryos. A delay in the hydration of the seeds may have caused decreased germination as a result of the reduced functionality of the aquaporins that were oxidized by mercury. In the presence of the reducing agent dithiothreitol, the activity of these proteins was restored and the germination process was re-established. These findings indicate the importance of aquaporins in the imbibition and germination stages of P. reticulata seeds, and they provide a better understanding of these important developmental events in plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins

et al. 2015

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“…For the desiccationtolerant organisms, drying is often a strategy to avoid rapid deterioration of living tissues and this implies several advantages. In the dry state, orthodox seeds, for example, can be distributed widely (Alpert 2005), since they can be stored for long periods, whereas recalcitrant seeds developed mechanisms for a quick germination (Barbedo & Marcos Filho 1998, Obroucheva et al 2012. In spite of not being immortal (Berjak 2006), seeds of some species can be stored for as long as thousands of years, such as Nelumbo nucifera Gaertn.…”

Section: Tolerance and Sensitivity To Desiccation Among Seeds Of Diffmentioning

confidence: 99%

Do recalcitrant seeds really exist?

Barbedo

Centeno

Ribeiro

2013

Hoehnea

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-(Do recalcitrant seeds really exist?). In the 70's, seeds were divided into two categories: recalcitrant and orthodox. In the 80's, it was necessary to create an intermediate category; from the 90's onwards, a gradient between orthodox and recalcitrant categories has been considered by several authors. Currently, the terms orthodox and recalcitrant are appropriate just for technological purposes, not for scientific studies. It seems that the differences between recalcitrant and orthodox seeds lie only on the maturity stage in which they are detached from the mother plant, the recalcitrant ones in a very immature stage. This implies that little progress should be expected to expand the storability of these recalcitrant seeds with the application of any treatment after harvesting. Efforts shall be focused on amplifying the maturation period of these seeds by keeping them linked to the mother plant until the maturation process has been completed. Key words: conservation, desiccation tolerance, maturation, seed storage RESUMO -(Sementes recalcitrantes realmente existem?). Na década de 1970, as sementes foram divididas em recalcitrantes e ortodoxas. Na década de 1980, foi necessário criar a categoria das intermediárias e, a partir de 1990, um gradiente entre as categorias ortodoxas e recalcitrantes tem sido considerado por vários autores. Atualmente, os termos ortodoxa e recalcitrante são apropriados apenas para fins tecnológicos, não para estudos científicos. As diferenças entre sementes recalcitrantes e ortodoxas parecem residir apenas no estádio de maturidade no qual se desprendem da planta mãe, as recalcitrantes em uma fase muito imatura. Isso implica que pouco progresso deve ser esperado para expandir a capacidade de armazenamento com a aplicação de qualquer tipo de tratamento após a colheita dessas sementes recalcitrantes. Esforços devem ser voltados para ampliar o período de maturidade das sementes, mantendo-as ligadas à planta-mãe até que o processo de maturação tenha sido concluído. Palavras-chave: armazenamento, conservação, maturação, tolerância à dessecação

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Protein profile of cotyledon, tegument, and embryonic axis of mature acorns from a non-orthodox plant species: Quercus ilex

Sghaier-Hammami

Redondo-López

Valero-Galván

et al. 2015

Planta

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Contrary to the orthodox seeds, recalcitrant Holm oak seeds possess the enzymatic machinery to start germination during the maturation phase. The protein profile of the different parts, mature seeds, of the Holm oak, a non-orthodox plant species, has been characterized using one- and two-dimensional gel electrophoresis coupled to matrix-assisted laser desorption ionization-time of flight mass spectrometry. Protein content and profiles of the three seed tissues (cotyledon, embryonic axis and tegument) were quite different. The embryonic axis showed 4-fold and 20-fold higher protein content than the cotyledon and the tegument, respectively. Two hundred and twenty-six variable proteins among the three seed parts were identified, being classified according to their function into eight main groups. The cotyledon presented the highest number of metabolic and storage proteins (89% of them are legumin) compared to the embryonic axis and tegument. The embryonic axis had the highest number of the species within the protein fate group. The tegument presented the largest number of the defense-/stress-related and cytoskeleton proteins. This distribution is in good agreement with the biological role of the tissues. The study of the seed tissue proteome demonstrated a compartmentalization of pathways and a division of metabolic tasks between embryonic axis, cotyledon and tegument. This compartmentalization uncovered in our study should provide a starting point for understanding, at the molecular level, the particularities of the recalcitrant seeds.

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Vacuolar status and water relations in embryonic axes of recalcitrant Aesculus hippocastanum seeds during stratification and early germination

Cited by 22 publications

References 38 publications

Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins

Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins

Do recalcitrant seeds really exist?

Protein profile of cotyledon, tegument, and embryonic axis of mature acorns from a non-orthodox plant species: Quercus ilex

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