Vacuum differential mobility spectrometry combined with column-switching liquid chromatography- mass spectrometry for the analysis of pyrrolizidine alkaloids in tea samples

Girard, Maria Fernanda Cifuentes; Knight, Patrick; Hopfgartner, Gérard

doi:10.1016/j.chroma.2023.464174

Cited by 4 publications

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High‐throughput liquid chromatography‐vacuum differential mobility spectrometry‐mass spectrometry for the analysis of isomeric drugs of abuse in human urine

Cifuentes Girard,

Knight,

Hopfgartner

2024

Drug Testing and Analysis

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The use of differential mobility spectrometry at low pressure coupled to liquid chromatography‐mass spectrometry (LC‐vDMS‐MS) was investigated for the analysis of 13 drugs of abuse (DoA) including the following: cocaine, ecgonine methyl ester, cocaethylene, benzoylecgonine, norcocaine, tramadol, isomeric pairs of metabolites; O‐desmethyl‐cis‐tramadol and N‐desmethyl‐cis‐tramadol, and cannabinoids: Δ9‐tetrahydrocannabinol, Δ9‐tetrahydrocannabidiol, 11‐hydroxy‐Δ9‐tetrahydrocannabinol, 11‐nor‐9carboxy‐Δ9‐tetrahydrocannabinol, and 11‐nor‐9carboxy‐Δ9‐tetrahydrocannabinol glucuronide. Different parameters were optimized for isomeric separation, such as LC mobile phase composition (20%–100% methanol acetonitrile and isopropanol, flow rate: 8–100 μL/min) and DMS separation voltage. Methanol and acetonitrile significantly affected the compensation voltage of the analytes and improved DMS separation. A short trap/elute LC‐vDMS‐SIM/MS screening method of 1 min was developed to quantify 11 drugs of abuse (except THC/CBD), in addition to a 4‐min LC‐vDMS‐SIM/MS method to identify and quantify five cannabinoids including the isomers THC/CBD and three THC metabolites. THC is the principal psychoactive constituent of cannabis and is a controlled substance in comparison to its isomeric counterpart CBD; this highlights the importance and challenges to resolve these isomeric pairs by analytical techniques. The signal responses were linear over a concentration range of 0.005–10 μg/mL for the DoA and 1–1000 ng/mL for cannabinoids. The intraday and interday precision were better than 12.2% and accuracy better than 115%. Urine samples from subjects who tested positive for THC and/or cocaine during roadside drug testing were evaluated to assess the performance of the methods LC‐vDMS‐SIM/MS and LC‐MRM/MS. Results show that the developed LC‐vDMS‐SIM/MS method presents similar performance to LC‐MRM/MS with improved sample throughput.

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