Validação do método de Ducos modificado para a determinação do ácido trans,trans-mucônico urinário

Paula, Flávia C. S. de; Silveira, Josianne Nicácio; Alvarez-Leite, Edna Maria

doi:10.1590/s1516-93322003000100006

Cited by 3 publications

(6 citation statements)

References 13 publications

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“…Moreover, it has been reported that the consumption of alcoholic beverages does not seem to have significantly influenced urinary t,t-MA values [ 10 ]. We have also found no association between levels of urinary t,t-MA and the alcoholic beverages.…”

Section: Discussionmentioning

confidence: 99%

“…Measurements of urine creatinine levels were performed after 12 h of collection, by Jaffe’s reaction, as determined by the end-point colorimetric method (Bioclin ® ). All urine samples that presented creatinine values lower than 0.3 g/L or greater than 3.0 g/L were excluded from analysis, following the guidelines of the American Conference of Governmental Industrial Hygienists (ACGIH) [ 10 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

“…The urinary levels of the t,t-MA were evaluated by high efficiency liquid chromatography, a method initially proposed by Ducos et al [ 27 ], modified by Paula [ 10 ] using a UV detector (HPLC-UV), equipped with a 5 μm Luna Phenomenex ® chromatography column (250 × 4.6 mm). The standard was obtained from Sigma-Aldrich ® and the normalization of results was adjusted by creatinine values.…”

Section: Methodsmentioning

confidence: 99%

“…The biotransformation of benzene in the liver generates a variety of hydroxylated products. At the end of this process, benzene epoxide conjugated to glutathione will generate the urinary metabolite S-phenylmercapturic acid (S-PMA) [ 9 , 10 , 11 ] and the trans-trans-mucaldehyde, and after the action of the enzymes alcohol dehydrogenase and aldehyde dehydrogenase, will generate trans,trans-muconic acid (t,t-MA). It is estimated that a variation of 2.0 to 3.9% of the absorbed benzene is excreted as t,t-MA in the urine, and that the elimination half-life of this metabolite is 5.1 h [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Benzene Exposure in Gas Station Workers Using Trans,Trans-Muconic Acid

Geraldino

Nunes

Gomes

et al. 2020

IJERPH

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In Brazil, gas station workers are occupationally exposed to the benzene present in gasoline. Brazilian law indicates the use of trans,trans-muconic acid(t,t-MA) as a biomarker of benzene exposure. The aim of this study was to evaluate the level of exposure to benzene in gas station workers, through the quantification of t,t-MA present in urine. A total number of 269 gas station workers divided into 179 filling station attendants exposed by inhalation and dermal route and 90 convenience store workers exposed only by inhalation were included. A control group was formed by 100 office workers, without occupational exposure to benzene. The urinary levels of t,t-MA were evaluated by HPLC with a UV detector. Gas station workers showed higher mean values of t,t-MA (0.204 mg/g creatinine; 95% CI 0.170–0.237) than office workers (0.126 mg/g creatinine; 95% CI 0.0817–0.1693). T,t-MA levels were higher in convenience store workers exposed to gasoline only by inhalation (0.221 mg/g creatinine; 95% CI 0.160–0.282), than in those exposed to gasoline by inhalation and dermal route—filling station attendants (0.195 mg/g creatinine; 95% CI 0.155–0.235). Gas station workers with a higher level of t,t-MA had epistaxis. T,t-MA values were higher in the Downtown (0.15 mg/g creatinine) region’s workers than in the more affluent South Zone region’s workers (0.07 mg/g creatinine). Smoking habits influenced the urinary t,t-MA values, while the frequency of consumption of industrialized and frozen foods showed no influence.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of Benzene Exposure in Gas Station Workers Using Trans,Trans-Muconic Acid

Geraldino

Nunes

Gomes

et al. 2020

IJERPH

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“…Destaca-se ainda que cada cartucho empacotado com o MIP apresentou uma vida útil de até 120 extrações, frente a apenas 3 permitidas pelo método de referência para extração de AttM, a troca iônica. 10,33 A precisão e exatidão intra e interdias foram avaliadas por meio de amostras de urina fortificada com concentração de AttM de 0,5; 3,0 e 7,0 mg L -1 analisadas no mesmo dia (n = 5) e em dias consecutivos (n = 3). Os resultados da precisão intra e interdia são apresentados na forma de desvio padrão relativo e erro relativo, respectivamente (Tabela 3).…”

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Extração de ácido trans-trans mucônico urinário com polímeros de impressão molecular e análise por cromatografia gasosa: espectrometria de massas

Franqui¹,

Vieira²,

Maia³

et al. 2012

Quím. Nova

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Recebido em 4/1/12; aceito em 17/4/12; publicado na web em 20/7/2012 MOLECULARLY IMPRINTED SOLID-PHASE EXTRACTION OF URINARY TRANS-TRANS MUCONIC ACID AND ANALYSIS BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY. This paper describes selective molecularly imprinted solid-phase extraction of ttMA from urine samples followed by derivatization and analysis by gas chromatography/mass spectrometry (GC/MS). The analytical calibration curve ranged from 0.3 to 7.0 mg L-1 (r = 0.999) and the limit of quantitation (LOQ) was 0.3 mg L-1. The method was applied for the determination of ttMA in urine samples from smokers and concentrations detected ranged from < LOQ to 1.64 mg L-1. Thus, the proposed method proved adequate for the determination of urinary ttMA in the biomonitoring of occupational exposure to low levels of benzene.

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Hematological Changes in Gas Station Workers

Giardini

Poça

Silva

et al. 2023

IJERPH

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(1) Background: Benzene, toluene, and xylene isomers (BTX) are present in gasoline. Exposure to benzene may lead to the appearance of a series of signs, symptoms, and complications, which are characterized by benzene poisoning, which is an occupational disease. This study evaluated the presence of signs and symptoms related to occupational exposure and whether occupational exposure to BTX is associated with the development of hematological changes. (2) Material and Methods: This cross-sectional epidemiological study included 542 participants, in which 324 were gas station workers (GSWs) and 218 were office workers (OWs) with no occupational exposure to benzene. To characterize the type of exposure (exposed and not exposed), trans,trans-Muconic acid (tt-MA), Hippuric acid (HA), and Methylhippuric acid (MHA) were used as exposure biomarkers. The tt-MA analysis revealed that the GSWs had 0.29 mg/g of urinary creatinine and the OWs had 0.13 mg/g of urinary creatinine. For HA, the GSWs presented 0.49 g/g of creatinine while the OWs presented 0.07. MHA analysis revealed that the GSWs had 1.57 g/g creatinine and the OWs had 0.01 g/g creatinine. Occupation habits and clinical symptoms were collected by questionnaire and blood samples were analyzed for hematological parameters. The persistence of hematological changes was evaluated with three serial blood collections every 15 days followed by laboratory hematological analysis. A descriptive analysis by the Chi-square test method was performed to evaluate the association between occupational exposure to fuels and the occurrence of changes in hematological parameters. (3) Results: In the GSWs, the most described signs and symptoms were somnolence (45.1%), headache (38.3%), dizziness (27.5%), tingling (25.4%), and involuntary movement (25%). Twenty GSWs that presented hematological alterations performed serial collections fifteen days apart. In addition, these workers presented total leukocyte counts above the upper limit and lymphocyte counts close to the lower limit. Leukocytosis and lymphopenia are hematological alterations present in chronic benzene poisoning. (4) Conclusions: The results found an initial change in different hematological parameters routinely used in clinics to evaluate health conditions. These findings reveal the importance of valuing clinical changes, even in the absence of disease, during the health monitoring of gas station workers and other groups that share the same space.

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Validação do método de Ducos modificado para a determinação do ácido trans,trans-mucônico urinário

Cited by 3 publications

References 13 publications

Analysis of Benzene Exposure in Gas Station Workers Using Trans,Trans-Muconic Acid

Analysis of Benzene Exposure in Gas Station Workers Using Trans,Trans-Muconic Acid

Extração de ácido trans-trans mucônico urinário com polímeros de impressão molecular e análise por cromatografia gasosa: espectrometria de massas

Hematological Changes in Gas Station Workers

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