Validated high performance liquid chromatography–UV detection method for the determination of daptomycin in human plasma

Martens‐Lobenhoffer, Jens; Kielstein, Jan T.; Oye, Catrin; Bode‐Böger, Stefanie M.

doi:10.1016/j.jchromb.2008.09.019

Cited by 56 publications

(30 citation statements)

References 14 publications

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“…This assay demonstrated an intraday coefficient of variance of less than 10% for all standards. Concentrations of daptomycin were determined using a validated high-performance liquid chromatography assay (34)(35)(36). This assay has demonstrated an interday coefficient of variation between 0.6 and 7.3% for all standards.…”

Section: Methodsmentioning

confidence: 99%

Fosfomycin Enhances the Activity of Daptomycin against Vancomycin-Resistant Enterococci in an In Vitro Pharmacokinetic-Pharmacodynamic Model

Snyder

Werth

Nonejuie

et al. 2016

Antimicrob Agents Chemother

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Section: Methodsmentioning

confidence: 99%

Fosfomycin Enhances the Activity of Daptomycin against Vancomycin-Resistant Enterococci in an In Vitro Pharmacokinetic-Pharmacodynamic Model

Snyder

Werth

Nonejuie

et al. 2016

Antimicrob Agents Chemother

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“…Antibiotic detection was performed at 220.5 nm for daptomycin and 280 nm for vancomycin. 11,12 The EE is expressed as the percentage of encapsulated antibiotic reported to the initial amount used for particle preparation. Drug loading (DL, %) was calculated according to the equation:…”

Section: Particle Characterizationmentioning

confidence: 99%

“…Briefly, quantification of daptomycin was performed using a Merck LiChospher 125-4, RP18 5 µm, LiChroCART 100 column and the following chromatographic conditions: 0.7 mL/min flow; injection volume of 20 µL; mobile phase of 35% acetonitrile and 65% PBS (pH 7.4), and detection at 230 nm. 11 As for vancomycin, the same type of column was used and the chromatographic conditions were as follows: 1.2 mL/min flow; injection volume of 40 µL; mobile phase of 10% acetonitrile and 90% H 2 KPO 4 (pH 2.75), and detection at 280 nm. 12 All samples were analyzed in triplicate.…”

Section: In Vitro Releasementioning

confidence: 99%

Activity of daptomycin- and vancomycin-loaded poly-epsilon-caprolactone microparticles against mature staphylococcal biofilms

Ferreira¹,

Bettencourt²,

Gonçalves³

et al. 2015

IJN

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The aim of the present study was to develop novel daptomycin-loaded poly-epsilon-caprolactone (PCL) microparticles with enhanced antibiofilm activity against mature biofilms of clinically relevant bacteria, methicillin-resistant Staphylococcus aureus (MRSA) and polysaccharide intercellular adhesin-positive Staphylococcus epidermidis . Daptomycin was encapsulated into PCL microparticles by a double emulsion-solvent evaporation method. For comparison purposes, formulations containing vancomycin were also prepared. Particle morphology, size distribution, encapsulation efficiency, surface charge, thermal behavior, and in vitro release were assessed. All formulations exhibited a spherical morphology, micrometer size, and negative surface charge. From a very early time stage, the released concentrations of daptomycin and vancomycin were higher than the minimal inhibitory concentration and continued so up to 72 hours. Daptomycin presented a sustained release profile with increasing concentrations of the drug being released up to 72 hours, whereas the release of vancomycin stabilized at 24 hours. The antibacterial activity of the microparticles was assessed by isothermal microcalorimetry against planktonic and sessile MRSA and S. epidermidis . Regarding planktonic bacteria, daptomycin-loaded PCL microparticles presented the highest antibacterial activity against both strains. Isothermal microcalorimetry also revealed that lower concentrations of daptomycin-loaded microparticles were required to completely inhibit the recovery of mature MRSA and S. epidermidis biofilms. Further characterization of the effect of daptomycin-loaded PCL microparticles on mature biofilms was performed by fluorescence in situ hybridization. Fluorescence in situ hybridization showed an important reduction in MRSA biofilm, whereas S. epidermidis biofilms, although inhibited, were not eradicated. In addition, an important attachment of the microparticles to MRSA and S. epidermidis biofilms was observed. Finally, all formulations proved to be biocompatible with both ISO compliant L929 fibroblasts and human MG63 osteoblast-like cells.

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“…Most of these studies are directed towards biological fluid analysis by high performance liquid chromatography (HPLC), (Tobin et al, 2008;Martens-Lobenhoffer et al, 2008;Baietto et al, 2010;Gika et al, 2010;Olszowy et al, 2010;Polillo et al, 2010;Verdier et al, 2011;Szultka et al, 2013) and ultra-performance liquid chromatography (UHPLC) (Gikas et al, 2010a; Gikas et al, 2010b; Bazoti et al, 2011;Baietto et al, 2014;Naito et al, 2014). In addition, there are no monographs of this drug in any official compendium.…”

Section: Introductionmentioning

confidence: 99%

A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry

Tótoli

Salgado

2015

Braz. J. Pharm. Sci.

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Daptomycin is the first approved drug from a new class of antimicrobials, the cyclic lipopeptides, and is a very important antimicrobial agent in current clinical practice. Currently, there are no "green" analytical methods described in the literature to analyze the typical pharmaceutical dosage form of daptomycin. Thus, the aim of this work was to validate an environment-friendly spectrophotometric method in the UV region, for the analysis of daptomycin as a lyophilized powder. Water was used as diluent and the analyses were carried out on a spectrophotometer at 221 nm. The method met all validation requirements of the ICH guidelines, over a concentration range of 6-21 µg mL -1 . A Student's t-test demonstrated that the proposed method was comparable to an HPLC method previously validated. Thus, the validated spectrophotometric method could quantify daptomycin in a powder form for injectable solutions, while being an economical, rapid, and "green" alternative for routine analysis in quality control.Uniterms: Antimicrobials/quality control. Daptomycin/determination. Spectrophotometry/UV region/ quantitative analysis. Spectrophotometry/green analytical method.A daptomicina é o primeiro membro aprovado de uma nova classe de antimicrobianos, os lipopeptídeos cíclicos, e é muito importante para a prática clínica atualmente. Não existem métodos analíticos "verdes" descritos na literatura para a análise da daptomicina na forma farmacêutica. Desta forma, o objetivo deste trabalho foi a validação de método espectrofotométrico na região do UV ambientalmente favorável para análise da daptomicina em pó liofilizado. A água foi escolhida como diluente e as análises foram realizadas em 221 nm. O método atendeu a todas as exigências de validação dos guias do ICH, na faixa de 6-21 µg mL -1 . Teste t de Student mostrou que o método proposto é intercambiável com método de HPLC previamente validado. Assim, o método espectrofotométrico validado é capaz de quantificar a daptomicina em pó para solução injetável e é uma opção econômica, rápida e "verde" para análises de rotina do controle de qualidade deste fármaco.Unitermos: Antimicrobianos/controle de qualidade. Daptomicina/determinação. Espectrofotometria/ região do UV/análise quantitativa. Espectrofotometria/método analítico verde. INTRODUCTIONIt is widely known that microbial resistance is a major problem worldwide. Many bacteria have developed resistance to a large number of antimicrobial agents available on the market; these bacteria are considered challenges for medicine. Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE) are two examples of antimicrobialresistant microorganisms (Silveira et al., 2006;Wright, 2011). Compounding the problem of antimicrobial resistance, there are few new antimicrobial agents emerging in the market (Wright, 2011).One recent development in antimicrobial treatment is the introduction of daptomycin into the marketplace (Figure 1). This is the first antimicrobial agent from the cyclic lipopeptide class appro...

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Validated high performance liquid chromatography–UV detection method for the determination of daptomycin in human plasma

Cited by 56 publications

References 14 publications

Fosfomycin Enhances the Activity of Daptomycin against Vancomycin-Resistant Enterococci in an In Vitro Pharmacokinetic-Pharmacodynamic Model

Fosfomycin Enhances the Activity of Daptomycin against Vancomycin-Resistant Enterococci in an In Vitro Pharmacokinetic-Pharmacodynamic Model

Activity of daptomycin- and vancomycin-loaded poly-epsilon-caprolactone microparticles against mature staphylococcal biofilms

A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry

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