Validated HPLC method for identification and quantification of p -hydroxy benzoic acid and agnuside in Vitex negundo and Vitex trifolia

Shah, S.; Dhanani, Tushar; Kumar, Satyanshu

doi:10.1016/j.jpha.2013.09.008

Cited by 22 publications

(19 citation statements)

References 12 publications

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“…The calibration curves indicated good linearity, with correlation coefficients of 1.000 for both compounds. The LOD of an individual analytical procedure represents the lowest amount of an analyte in a sample that can be detected but not necessarily quantified, while the LOQ of an individual analytical procedure represents the lowest amount of analyte in a sample that can be determined with suitable precision and accuracy . The LOD values of CA and KA were 0.068 and 0.097 μg/mL, respectively, and their corresponding LOQ values were 0.207 and 0.295 μg/mL, demonstrating high sensitivity under the utilized chromatographic conditions (Table ).…”

Section: Resultsmentioning

confidence: 99%

Ultra‐performance convergence chromatography for the quantitative determination of bioactive compounds in Aralia continentalis Kitagawa as quality control markers

Kim

Moon

Choi

et al. 2017

J of Separation Science

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A rapid ultra-performance convergence chromatography method was developed for the quantitative determination of bioactive compounds in Aralia continentalis as quality control markers. Quantitative analysis indicated the presence of two major bioactive compounds: diterpenoid acids continentalic acid and kaurenoic acid. Using a Torus 1-aminoanthracene column, continentalic acid and kaurenoic acid were separated in less than 8 min. The method was validated with respect to precision, accuracy, and linearity according to the International Conference on Harmonization guidelines. The optimized method exhibited a good linear correlation (r > 0.996), excellent precision (RSD< 1.0%), and acceptable recoveries (99.97-100.26%). Limits of detection for continentalic acid and kaurenoic acid were 0.068 and 0.097 μg/mL, respectively, while their corresponding limits of quantitation were 0.207 and 0.295 μg/mL. The system performance of ultra-performance convergence chromatography was compared with that of conventional high-performance liquid chromatography with respect to analysis time and efficiency. The proposed method was found to be reliable and convenient for the quantitative analysis of continentalic acid and kaurenoic acid in A. continentalis from South Korea and A. pubescens from China. This study is expected to serve as a guideline for the quality control of Aralia continentalis.

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Section: Resultsmentioning

confidence: 99%

Ultra‐performance convergence chromatography for the quantitative determination of bioactive compounds in Aralia continentalis Kitagawa as quality control markers

Kim

Moon

Choi

et al. 2017

J of Separation Science

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“…The IUPAC name of 2 was established as [5-hydroxy-1-[3,4,5-trihydroxy-6-(hydroxymethyl)-oxan-2-yl]oxy-1,4a,5,7a-tetrahydrocyclopenta[c]pyran-7-yl]methyl 4-hydroxy-benzoate. Hence, compound 2, was proposed as a known composition of V. trifolia and it is commercially available [16]. The occurrences of 2 were also reported in other Vitex, including V. cannabifolia, V. rotundifolia, V. cymosa, V. agnus-castus and V. negundo.…”

Section: Isolation and Purification Of The Vitex Extractmentioning

confidence: 94%

“…The quantification of 2 via high performance liquid chromatography (HPLC) proposed that the leaves contained relatively high amount of 2. Therefore, the bark part should not be disrupted, thus setting the efforts in conserving the plant [16].…”

Section: Isolation and Purification Of The Vitex Extractmentioning

confidence: 99%

Vitex Species: Review on Phytochemistry and Pouch Design for Nutritional Benefits

et al. 2016

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Vitex trifolia or locally known as lemuni, is well documented for its medicinal properties including anticancer, antibacterial and wound healing. For years, the leaves and flowers were consumed for anti-aging and general well-being. It is also commonly served as nutraceuticals to post-partum women during their confinement period. Previous study showed that V. trifolia possesses high antioxidant properties that could prevent oxidative stress related diseases such as cancer, atherosclerosis, diabetes and many more. From the literature, V. trifolia leaves are used as poultice for rheumatic pains, sprains, antithelmintics and inflammations. Meanwhile, the fruits are utilised in amenorrhea. The roots, on the other hand, are used for the treatment of cough and fever. The plant is known to possess pharmacologically-active constituents such as the essential oil, halimane-type diterpenes, flavonoids, triterpenes, lignans, iridoids and ecdysteroids. Review papers were consistently published to justify the non-exhaustive biological exploration of this genus. Nevertheless, only 11 per cent of the total Vitex species were phytochemically examined. Apart from being eaten raw or blanched, Vitex is also taken as a food colourant in preparing lemuni rice. The process of making lemuni rice is quite tedious. First, the leaves and flowers are picked and washed. Both parts are then ground with water and sieved to give a bluish extract which is then mixed with rice and boiled to cook. Hence, the Vitex pouch is introduced to provide an easy way to prepare lemuni rice. This packaging is designed to enclose Vitex samples in a pouch that simply can be placed together with rice and boiled to cook. Such instant preparation will hopefully promote the consumption of lemuni rice among busy locals. Future plans include international marketing and production of other Vitex nutritional products.

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“…Linearity was assessed over a concentration range of 0.1-50 µg/mL at seven different concentration levels. The intra-and inter-day variability was confirmed by calculating % bias from the theoretical concentration using equation: % bias = (observed concentration -nominal concentration)/nominal concentration × 100) [21]. The intra-and inter-day precisions were calculated by subjecting the data to the one-way analysis of variance respective to their % RSD.…”

Section: Methods Validationmentioning

confidence: 99%

Determination of 5h-Benzo[2,3][1,4]oxazepino[5,6-B]indoles in Rat Plasma by Reversed-Phase High-Performance Liquid Chromatographic-Ultraviolet Method: Application to Pharmacokinetic Studies

Singh

Raj

Rai

et al. 2017

Asian J Pharm Clin Res

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Objective: Recently, we reported newly synthesized 5H-benzo [2,3] [1,4]oxazepino [5,6-b]indole) derivatives and proved their cytotoxicity against hepatocellular carcinoma specific Hep-G2 cell lines. We attempted herein to describe a reversed-phase high-performance liquid chromatographic method for the determination of three most active compounds 6a, 10a, and 15a in rat plasma to predict their pharmacokinetics parameters before in vivo study. Methods:A rapid and sensitive reversed-phase high-performance liquid chromatographic was employed for the determination of 6a, 10a, and 15a in rat plasma. Each compound was separated by a gradient elution of acetonitrile and water with 1 mL/min flow rate. The detector was set at 270, 285, and 275 nm for 6a, 10a, and 15a and the recorded elution times were 2.00, 2.87, and 1.88 min, respectively. Results:The calibration curve was linear with R 2 of 0.938, 0.875, and 0.923 over the concentration range of 0.1-50 µg/mL. The inter-and intraday variations of the assay were lower than 12.26%; the average recovery of 6a, 10a, and 15a was 97.31, 92.56, and 95.23 % with relative standard deviation of 2.12%, 3.25%, and 2.28%, respectively. The C max and T max were ~ 46.34, 18.56, and 25.65 µg/mL and 2.0, 4.0, and 4.0 h for 6a, 10a, and 15a, respectively, which indicate a robust method of detection in the present experiment. Conclusion:The study suggests that all of the three compounds have a lower rate of absorption, higher volume of distribution, and lower clearance rate, indicating good therapeutic response for in vivo activity.

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Validated HPLC method for identification and quantification of p -hydroxy benzoic acid and agnuside in Vitex negundo and Vitex trifolia

Cited by 22 publications

References 12 publications

Ultra‐performance convergence chromatography for the quantitative determination of bioactive compounds in Aralia continentalis Kitagawa as quality control markers

Ultra‐performance convergence chromatography for the quantitative determination of bioactive compounds in Aralia continentalis Kitagawa as quality control markers

Vitex Species: Review on Phytochemistry and Pouch Design for Nutritional Benefits

Determination of 5h-Benzo[2,3][1,4]oxazepino[5,6-B]indoles in Rat Plasma by Reversed-Phase High-Performance Liquid Chromatographic-Ultraviolet Method: Application to Pharmacokinetic Studies

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