Validated method for estimation of irbesartan in bulk and dosage form by high performance liquid chromatography technique

Kulkarni, NS; Rathor, D.S.; Ranpise, Nisharani S.; Dhole, S. N.

doi:10.30750/ijpbr.3.3.7

Cited by 3 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This decrease in the zeta potential indicates that the xanthan gum polysaccharide matrix, through either the interaction of different functional groups with bacterial surface or aggregation within the dispersion, resulted in the perturbation of the surface charge density of the B. longum [45]. It is known that dispersion or suspension with a low zeta potential value promotes the aggregation of particles due to van der Waals attractions [46], which was also observed in this case as confirmed by the higher z-average value. However, this did not affect the stability of the dispersion as the absolute value above 25 mV and the zeta potential is generally considered an indication that the particle suspension will be electrostatically stabilized [47].…”

Section: Discussionmentioning

confidence: 99%

Utilizing Xanthan Gum Coatings as Probiotic Bacteria Carriers to Enhance Postharvest Quality and Antioxidants in Fresh-Cut Cantaloupe and Honeydew (Cucumis melo L.) Melons

Chikhala,

Seke,

Slabbert

et al. 2024

Foods

View full text Add to dashboard Cite

Due to spoilage microflora and browning, minimally processed fresh-cut fruits have a short shelf life, and over the years, studies have shown the potential of using edible coatings to extend the shelf life and improve the safety of fresh-cut fruits. Recently, there has been a rise in research on the incorporation of probiotics in edible coatings due to the bespoke health and biopreservation benefits they impart. Therefore, in this study, lactobacillus strains (Lactiplantibacillus plantarum 75 and Bifidobacterium longum) were incorporated into a xanthan edible coating to enhance color retention, sensory properties, antioxidant retention (ascorbic acid, carotenoids, total phenols), and antioxidant activity (FRAP antioxidant power, ABTS scavenger activity) of fresh-cut cantaloupes and honeydew cucumis melo L. melons during cold storage at 5 C and 85% RH for five days. The edible coating was prepared by mixing 0.5% xanthan gum, 1% glycerol, and 2% citric acid solution with L. plantarum 75 (LAB 75) and Bifidobacterium longum bacteria separately, and the final lab count for each strain was made to be 8.0 log CFU/mL. Stable probiotic coatings with ζ-potential of between −39.7 and −51.4 mV and a PdI of 1 were developed, and the incorporation of the probiotic bacteria into the coating was justified using FTIR analysis. The probiotic coatings showed a typical pseudoplastic behavior, in which the viscosity curves fall as the shear rate increases. Thermal stability analysis showed a continuous and multi-step weight reduction in this work, illustrating how the edible coating components interact. The survival of both Lactobacillus strains was recorded on day 5. Both freshly cut melons coated with xanthan and loaded with Lactobacillus strains retained a sufficient quantity of probiotics at the end of storage, while L. plantarum 75 (7 log CFU/g for cantaloupe and 8 log CFU/g for honeydew) retained the highest viability compared to B. longum (6 log CFU/g for cantaloupe and 7 log CFU/g for honeydew). In comparison to the coated and uncoated control samples, the inclusion of L. plantarum 75 in xanthan coatings significantly retained the color properties, pigments (total chlorophyll and carotenoids), ascorbic acid, total phenols, and antioxidant activity (FRAP, DPPH, and ABTS). The overall acceptability of fresh cuts of cantaloupe and honeydew melons coated with xanthan gum loaded with L. plantarum 75 was higher than that of other treatments. Thus, xanthan gum loaded with L. plantarum 75 coating is most suitable for reducing postharvest losses in fresh cuts of honeydew melons and cantaloupe, which will help preserve antioxidant and bioactive properties. The xanthan gum loaded with L. plantarum 75 coatings exhibited the highest preservation impact; therefore, it can be recommended for the fresh-cut industry.

show abstract

Section: Discussionmentioning

confidence: 99%

Utilizing Xanthan Gum Coatings as Probiotic Bacteria Carriers to Enhance Postharvest Quality and Antioxidants in Fresh-Cut Cantaloupe and Honeydew (Cucumis melo L.) Melons

Chikhala,

Seke,

Slabbert

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

“…Particles were thought to be stable when their zeta potential was either 30 mV or less, making any of these values acceptable [ 39 ]. All BPO nanoemulgel formulations exhibited a zeta potential for the drug lower than −35 mV owing to the presence of non-ionic surfactants, which stabilize the system by forming a coating over the surface of the nanoparticles [ 40 ]. They do not contribute to any change in the nanoemulsion particle.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Anti-Acne Activity of Benzoyl Peroxide Nanoparticles Incorporated in Lemongrass Oil Nanoemulgel

Eid

Naseef

Jaradat

et al. 2023

Gels

View full text Add to dashboard Cite

Purpose: The goal of this study was to make Benzoyl Peroxide (BPO) nanoemulgel to improve its ability to kill bacteria. BPO has trouble getting into the skin, being absorbed by the skin, staying stable, and being spread out. Methods: A BPO nanoemulgel formulation was prepared by combining BPO nanoemulsion with Carbopol hydrogel. The drug was tested for solubility in various oils and surfactants in order to select the best oil and surfactant for the drug, and then the drug nanoemulsion formulation was prepared using a self-nano-emulsifying technique with Tween 80, Span 80, and lemongrass oil. The drug nanoemulgel was looked at in terms of its particle size, polydispersity index (PDI), rheological behavior, drug release, and antimicrobial activity. Results: Based on the solubility test results, lemongrass oil was the best solubilizing oil for drugs, while Tween 80 and Span 80 demonstrated the highest solubilizing ability for drugs among the surfactants. The optimum self-nano-emulsifying formulation had particle sizes of less than 200 nm and a PDI of close to zero. The results showed that incorporating the SNEDDS formulation of the drug with Carbopol at various concentrations did not cause a significant change in the particle size and PDI of the drug. The zeta potential results for drug nanoemulgel were negative, with more than 30 mV. All nanoemulgel formulations exhibited pseudo-plastic behavior, with 0.4% Carbopol exhibiting the highest release pattern. The drug nanoemulgel formulation worked better against bacteria and acne than the product on the market. Conclusion: Nanoemulgel is a promising way to deliver BPO because it makes the drug more stable and increases its ability to kill bacteria.

show abstract

“…Irbesartan is used mainly for the treatment of hypertension. Irbesartan pronounced is an angiotensin II receptor antagonist [1][2][3][4][5][6][7] . IAPUC name of Irbesartan is 2-butyl-3-({4-[2-(2H-1, 2, 3, 4 -tetrazol-5-yl) phenyl] phenyl} methyl) -1, 3 -diazaspironon-1-en-4-one and molecular formula C 25 H 28 N 6 O [8][9][10][11][12][13] .…”

mentioning

confidence: 99%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

ABSTRACT:A stability indicating RP-HPLC method was developed and validated for the determination of Irbesartan in bulk and dosage forms using Telmisartan (10 μg/ml) as the internal standard. An Inert ODS C-18, 5μm column having 250 x 4.6mm internal diameter in isocratic mode with mobile phase containing methanol: water (90:10) and the pH was adjusted to 3 with 1 % GAA. The flow rate was 1 ml/min and effluents were monitored at the wavelength of 246 nm. The retention time for Irbesartan was 2.3 min. The method was validated as per ICH guidelines for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of detection (LOD) and limit of quantification (LOQ) were found 6.51μg/ml and 1.973μg/ml respectively and recovery of Irbesartan from bulk and dosage forms was found from 99.94% to 99.97%. As the separation of the degradants using this mobile phase is quite good, isolation of the degradants with preparative techniques can also be achieved using this mobile phase. The drug was prone to degrade more in acidic, alkaline, oxidative and thermal conditions. Further LC MS-MS analysis will help to deduce the structures of the degradants which can help to establish the possible degradation pathway of this drug. So this method can be economically very useful in both research and industrial aspect. INTRODUCTION:Angiotensin antagonists are the first major innovation in essential hypertension management as a first-line treatment. Angiotensin II receptor antagonists have been developed to specifically and selectively block the AT1 receptor of the rennin angiotensin system by displacing angiotensin II from it. Losartan potassium, Telmisartan and Irbesartan are highly selective, non-peptide angiotensin-II receptor antagonists (ARA-II).

show abstract

Validated method for estimation of irbesartan in bulk and dosage form by high performance liquid chromatography technique

Cited by 3 publications

References 3 publications

Utilizing Xanthan Gum Coatings as Probiotic Bacteria Carriers to Enhance Postharvest Quality and Antioxidants in Fresh-Cut Cantaloupe and Honeydew (Cucumis melo L.) Melons

Utilizing Xanthan Gum Coatings as Probiotic Bacteria Carriers to Enhance Postharvest Quality and Antioxidants in Fresh-Cut Cantaloupe and Honeydew (Cucumis melo L.) Melons

Antibacterial and Anti-Acne Activity of Benzoyl Peroxide Nanoparticles Incorporated in Lemongrass Oil Nanoemulgel

Untitled

Contact Info

Product

Resources

About