Validated method for the quantitation of quercetin from human plasma using high-performance liquid chromatography with electrochemical detection

Erlund, Iris; Alfthan, Georg; Sirén, Heli M. M.; Ariniemi, Kari; Aro, Antti

doi:10.1016/s0378-4347(99)00049-3

Cited by 79 publications

(52 citation statements)

References 22 publications

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“…Usually, the time needed for this is four to five elimination half-lives. For quercetin, half-lives between 15 and 28 h have been reported (Hollman et al, 1997;Erlund et al, 1999), which indicates that steady-state levels are reached within 3 -6 days. However, to our knowledge, no studies actually showing that this applies to quercetin have been performed and most bioavailability or pharmacokinetics studies with quercetin have involved one-time ingestion of quercetinrich foods or supplements.…”

Section: Discussionmentioning

confidence: 99%

“…Serum quercetin concentrations were analyzed using a validated method developed at our laboratory (Erlund et al, 1999). In this method potential conjugates of quercetin are hydrolyzed and therefore the results represent total quercetin (unconjugated quercetin, quercetin conjugated with glucuronic acid, sulfate or glycoside groups, and quercetin either bound or not bound to protein).…”

Section: Intake Of Quercetinmentioning

confidence: 99%

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Consumption of black currants, lingonberries and bilberries increases serum quercetin concentrations

et al. 2003

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Objective: To study serum quercetin concentrations of subjects consuming berries or habitual Finnish diets. Design: Randomized parallel dietary intervention. Subjects: Forty healthy men (age 60 y). Intervention: Twenty subjects consumed 100 g=day of berries (black currants, lingonberries and bilberries) for 8 weeks. Twenty subjects consuming their habitual diets served as controls. Fasting blood samples were obtained 2 weeks prior to the study, at baseline, and at 2, 4 and 8 weeks. Intake of quercetin was assessed from 3 day food records collected at baseline and at 8 weeks.Results: The serum quercetin concentrations were significantly higher in the subjects consuming berries compared to the control group (P ¼ 0.039 ANCOVA with repeated measures). During the berry consumption period the mean serum concentrations of quercetin ranged between 21.4 and 25.3 mg=l in the berry group, which was 32 -51% higher compared with the control group. According to 3 day food records, there was no difference in quercetin intake at baseline, but at 8 weeks the intake was 12.3 AE 1.4 mg=day (mean AE s.e.m.) in the berry group and 5.8 AE 0.6 mg=day in the control group (P ¼ 0.001). Conclusions:The results indicate that the berries used in this study are a good source of bioavailable quercetin.

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Section: Discussionmentioning

confidence: 99%

Section: Intake Of Quercetinmentioning

confidence: 99%

Consumption of black currants, lingonberries and bilberries increases serum quercetin concentrations

et al. 2003

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“…The serum was separated immediately and was kept frozen at 7 70 C until analysed. Plasma flavonoid concentrations were analysed as reported previously (Erlund et al, 1999(Erlund et al, , 2001). In brief, 0.5 ml of plasma was incubated with 55 ml of 0.78 mol=l sodium acetate buffer (pH 4.8), 50 ml of 0.1 mol=l ascorbic acid and 20 ml of a crude enzyme preparation from Helix pomatia (type HP-2, Sigma), for 17 h at 37 C. Flavonoids were extracted from plasma proteins using Bond Elut C 18 solid-phase extraction columns and they were analyzed by reversed-phase high-performance liquid chromatography with electrochemical detection.…”

Section: Analysis Of Plasma Flavonoidsmentioning

confidence: 99%

Plasma concentrations of the flavonoids hesperetin, naringenin and quercetin in human subjects following their habitual diets, and diets high or low in fruit and vegetables

et al. 2002

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Objectives: To determine the fasting plasma concentrations of quercetin, hesperetin and naringenin in human subjects consuming their habitual diets, and diets either high or low in fruit and vegetables. To investigate whether plasma concentrations of flavanones can serve as biomarkers of their intake. Design: This was a cross-over, strictly controlled dietary intervention consisting of a 2 week baseline period, and two 5 week dietary periods with a 3 week wash-out period in between. The low-vegetable diet contained few fruit and vegetables and no citrus fruit. The high-vegetable diet provided various fruits and vegetables daily including on average one glass of orange juice, one-half orange and one-half mandarin. Subjects: Thirty-seven healthy females. Results: The high-vegetable diet provided 132 mg of hesperetin and 29 mg of naringenin. The low-vegetable diet contained no flavanones. The mean plasma hesperetin concentration increased from 12.2 nmol=l after the low-vegetable diet to 325 nmol=l after the high-vegetable diet. The respective increase for naringenin was from < 73.5 nmol=l for all subjects to a mean value of 112.9 nmol=l. The mean plasma quercetin concentration was 52 nmol=l after the baseline period, during which habitual diets were consumed, and it did not change significantly during the intervention. Interindividual variation in the plasma levels of hesperetin and naringenin was marked and, after the baseline and wash-out periods, and the low-vegetable diet, a majority of the samples had plasma flavanone levels below the limit of detection. After the high-vegetable diet, hesperetin and naringenin were detectable in 54 and 22% of all samples. Quercetin was detectable in nearly all samples after all study periods. Conclusion: Hesperetin, naringenin and quercetin are bioavailable from the diet, but the plasma concentrations of hesperetin and naringenin are poor biomarkers of intake.

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“…To determine and quantify these estrogen-like compounds, various HPLC methods have been developed such as ultraviolet detection, 10) photodiode array detection 11) and electrochemical detection. 12) In particular, a coulometric array system with multiple cells enabled us to determine a chemical's voltage-dependent profile besides its retention time. [13][14][15] This method makes it easier to distinguish a test chemical, even in the presence of contaminating substances with a similar retention time.…”

mentioning

confidence: 99%

Simultaneous Determination of Isoflavones and Bisphenol A in Rat Serum by High-performance Liquid Chromatography Coupled with Coulometric Array Detection

Yasuda¹,

Wu²,

Hattori³

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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Validated method for the quantitation of quercetin from human plasma using high-performance liquid chromatography with electrochemical detection

Cited by 79 publications

References 22 publications

Consumption of black currants, lingonberries and bilberries increases serum quercetin concentrations

Consumption of black currants, lingonberries and bilberries increases serum quercetin concentrations

Plasma concentrations of the flavonoids hesperetin, naringenin and quercetin in human subjects following their habitual diets, and diets high or low in fruit and vegetables

Simultaneous Determination of Isoflavones and Bisphenol A in Rat Serum by High-performance Liquid Chromatography Coupled with Coulometric Array Detection

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