Simultaneous Determination of Isoflavones and Bisphenol A in Rat Serum by High-performance Liquid Chromatography Coupled with Coulometric Array Detection

Ileal and faecal digestibility of daidzein and genistein and plasma bioavailability of these isoflavones and their bioactive metabolites in the ovariectomised rat

“…As endogenous enzymes are believed incapable of synthesising o-DMA, the presence of 2-dehydro-o-DMA in urine indicates it was absorbed from the intestine. Plasma concentrations of genistein, daidzein and equol in the present study were similar to those reported in other studies in rats [34,35]. Circulating concentrations of genistein in animals receiving the genistein-supplemented diet were not significantly different from those of daidzein in animals receiving the daidzein supplemented diet, indicating that the bioavailabilities of the unmetabolised phytoestrogens are similar.…”

Application of coulometric electrode array detection to the analysis of isoflavonoids and lignans

“…Based on the method of Gamache and Acworth [22], Nurmi and Adlercreutz published a method for plasma phytoestrogens, in which the conditions for the quantification of up to 13 compounds including lignans, isoflavones and two isoflavone metabolites were optimized for quantification of non supplemented samples by means of sample concentration during the sample pre-treatment [45]. Recently and also adapted from the above mentioned original methods Yasuda et al reported a protocol for the simultaneous determination of isoflavonoids and bisphenol A, an industrial chemical classified as a xenoestrogen, in rat serum [52]. The method includes the optimization of the conditions for quantification of Din, Glyn and Gin in serum which does not seem to agree with the already known lack of absorption of isoflavone glycosides [51].…”

Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection

“…A lot of chromatographic procedures for more or less efficient separation of isoflavones have been described in the literature [17,28,[45][46][47][48][49], but only part of them applied an HPLC-ED hyphenated technique [24,37,38,[50][51][52][53][54]. Recently, we have published highly efficient and sensitive methods for separation of particular isoflavones in several plant species [34,39,40].…”