Validated RP-HPLC Method for the Determination of Zolmitriptan - A Serotonin 5-HT Receptor Agonist

Annapurna,

doi:10.6000/1927-5951.2011.01.01.03

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…The calibration curve for Zolmitriptan was linear from 1 to 100μg/ml. The values of LOD & LOQ were 0.2687μg/ml & 0.8134μg/ml respectively 6 .…”

Section: Pharmacokineticsmentioning

confidence: 94%

Untitled

2022

IJPSR

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Zolmitriptan is a selective serotonin receptor agonist of 1B and 1D receptors belonging to the triptan class. Zolmitriptan binds to the serotonin (5-HT) 1B receptors, receptor binding leads to inhibition of nociceptive transmission, constriction of cranial vessels, and reduction of vessel pulsation, thereby providing relief of migraine headaches, as Zolmitriptan holds great importance in migraine headaches, it is necessary to compile the various analytical methods that have been reported in the literature for its analysis in pharmaceuticals or bulk products and in bioanalysis. Analytical techniques are used for pharmaceutical analysis and therapeutic drug monitoring to study bioavailability and bioequivalence. Most of the analytical methods reported for its determination are the ones that utilize HPLC and other hyphenated techniques like LC-MS, UPLC-MS, LC-MS/MS. Therefore, the present review provides a summary of the HPLC-based methods used in the determination and quantification of Zolmitriptan in different matrices since the time of its discovery.

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“…The calibration curve for Zolmitriptan was linear from 1 to 100μg/ml. The values of LOD & LOQ were 0.2687μg/ml & 0.8134μg/ml respectively 6 .…”

Section: Pharmacokineticsmentioning

confidence: 94%

Untitled

2022

IJPSR

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“…The reported methods in the literature suffer from one or the other disadvantage such as poor sensitivity, very narrow linearity range, scrupulous control of experimental variables and the present study reports the development and validation of a liquid chromatographic method with better detection ranges in pure form and its dosage forms. An LC-MS method was developed to determine antimigraine compounds but limit of quantification was 0.3 mg/mL which is not sensitive enough for studies [15]. Literature survey reveals that there was no method reported using isocratic RP-HPLC (without using buffers) for estimation of Zolmitriptan in its Nasal spray formulation [16].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of HPLC Method for Estimation of Zolmitriptan in its Pharmaceutical Dosage Form

Champaneria¹,

Gharia²,

Mishra³

et al. 2019

Pharm Anal Acta

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A new simple, accurate and precise HPLC method have been developed and validated for estimation of Zolmitriptan in its pharmaceutical dosage form. In RP-HPLC method, a C18 column and methanol: water in the ratio of 75:25 (v/v %), pH adjusted to 3 using 10% orthophosphoric acid were used at a flow rate of 1.0 mL/min and detected at 222 nm. The retention time for zolmitriptan was found to be 3.6 min. The developed method was validated for linearity, precision, accuracy, specificity, LOD and LOQ as per ICH guidelines. Linearity was observed in the range of 10-50 μg/mL for zolmitriptan and correlation coefficient was found to be 0.9979. LOD and LOQ for Zolmitriptan were found to be 2.84 μg/mL and 8.62 μg/mL respectively. The % recovery was found to be 99.87%-101.57%. The method was applied for estimation of Zolmitriptan in its pharmaceutical dosage form. The assay result was found to be 95.98 ± 1.82 of percentage label claim of Zolmitriptan. aliquot of 0.1, 0.2, 0.3, 0.4 and 0.5 mL were transferred in series of 10 mL volumetric flask and diluted up to mark with mobile phase to get the Figure 1: HPLC Instrument.FT-IR spectrophotometer: BRUKER, Alpha, Electronic analytical balance (Shimadzu, AUX-220) were used (Figure 1). Reagents and materialsPharmaceutical grade of Zolmitriptan was supplied as a gift sample. Methanol was used of LR grade and purchased from s.d. Fine Chem Limited, Mumbai, India. Zolmitriptan nasal spray (ZOLMIST) was procured from the local market. Preparation of standard solutionsPreparation of stock solution of ZMT: 25 mg of Zolmitriptan was weighed and transferred in 25 mL volumetric flask and volume made up to mark with methanol (1000 μg/mL). Preparation of working standard solution:From stock solution of ZMT,

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“…Most of the reported methods [8][9][10][11][12][13][14][15][16][17][18][19][20][21] require expensive instrumental setup, expertise personnel, and complicated procedure. Two of the reported visible spectrophotometric methods [23] require liquid-liquid extraction and strict pH control.…”

Section: Introductionmentioning

confidence: 99%

“…High-performance liquid chromatography (HPLC) with UV-detection has been widely used for the quantitative determination of ZMT in pharmaceuticals [8][9][10][11][12][13][14][15]. Ultraperformance liquid chromatography (UPLC) [16], liquid chromatography-mass spectrometry [17], voltammetry [18], and UV-spectrophotometry methods [19][20][21] are the other techniques applied for the assay of ZMT in pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Determination of Zolmitriptan in Bulk Drug and Pharmaceuticals Using Vanillin as a Reagent

Prashanth

Basavaiah

Raghu

2013

ISRN Analytical Chemistry

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An accurate and precise spectrophotometric method is presented for the determination of zolmitriptan (ZMT) based on the formation of a red color product with vanillin in presence of concentrated H 2 SO 4 , with the chromogen being measured at 580 nm. The reaction proceeds quantitatively at room temperature in 10 min. The calibration curve is linear over the range 5.0-90.0 g mL and described by the regression equation = (−)0.0101+0.0117 with a regression coefficient ( ) of 0.9994 ( = 7). The calculated molar absorptivity and Sandell sensitivity values are 3.3 × 10 3 L mol −1 cm −1 and 0.0872 g cm −2 , respectively. The limits of detection (LOD) and quantification (LOQ) calculated as per ICH guidelines are 1.26 and 3.81 g mL −1 , respectively. The within-day accuracy expressed as relative error was better than 1.78% with precision (RSD) ranging from 0.83 to 1.45%. The between-day accuracy ranged from 1.21 to 1.84% with a precision less than 1.66%. The method was successfully applied to the analysis of one brand of tablet containing zolmitriptan. The results obtained were in agreement with those obtained by published reference method. The accuracy was also checked by placebo blank and synthetic mixture analyses besides recovery study via standard addition procedure.

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Validated RP-HPLC Method for the Determination of Zolmitriptan - A Serotonin 5-HT Receptor Agonist

Cited by 7 publications

References 16 publications

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Development and Validation of HPLC Method for Estimation of Zolmitriptan in its Pharmaceutical Dosage Form

Spectrophotometric Determination of Zolmitriptan in Bulk Drug and Pharmaceuticals Using Vanillin as a Reagent

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