Validated simultaneous determination of antipyrine and benzocaine HCl in the presence of benzocaine HCl degradation product

Merey, Hanan A.; Zaazaa, Hala E.

doi:10.1039/c3ay42170h

Cited by 11 publications

(11 citation statements)

References 19 publications

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“…By reviewing the literature it was revealed that, only two articles have been published as stability indicating methods for determination of benzocaine and antipyrine in the presence of degradation product of BE 29,30 . No spectrophotometric methods were reported for the simultaneous analysis of AN and BE, this attract our attention to develop simple, rapid, precise and validated spectrophotometric methods for the simultaneous determination of AN and BE in pure form or in pharmaceutical formulation.…”

Section: Resultsmentioning

confidence: 97%

“…Good results were obtained (Tables 1-3). Statistical comparison between the results obtained by the proposed spectrophotometric methods and the reported method 30 for the determination of pure antipyrine and benzocaine in pure powder form was also done. The values of calculated t and F are less than the tabulated ones, which reveals that there is no significant difference between the suggested methods with respect to accuracy and precision, (Table 4).…”

Section: Accuracymentioning

confidence: 99%

“…Purity of AN and BE was found to be 100.22 ± 1.375 and 99.47 ± 1.071 according to its reported method 30. 2.3.2.…”

mentioning

confidence: 94%

“…28 Only two HPLC methods were elaborated for the determination of benzocaine and antipyrine in the presence of BE degradation product. 29,30 One TLC method was reported for the determination of benzocaine and antipyrine in the presence of BE degradation product. 30 From literature in hand, no spectrophotometric methods were used for the simultaneous determination of AN and BE, therefore the aim of this work was to develop and validate simple, precise, rapid and accurate spectrophotometric methods for the simultaneous determination of AN and BE in pure form or in pharmaceutical formulation.…”

Section: Introductionmentioning

confidence: 99%

“…*Figure betweenparenthesis are the corresponding tabulated values (p = 0.05). a Ref [30]. HPLC method using C 8 (5 lm, 4.6 Â 150 mm ID), mobile phase consisted of acetonitrile-phosphate buffer of pH 5.5 (25:75, v/v) and UV detection at 270 nm.…”

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confidence: 99%

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Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine

Merey

2016

Bulletin of Faculty of Pharmacy, Cairo University

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Antipyrine and benzocaine are formulated together for the treatment of ear inflammation and to relieve pain. Four spectrophotometric methods were developed for the simultaneous determination of antipyrine (AN) and benzocaine (BE) in their combined dosage form. Method A depends on applying dual wavelength method where antipyrine was determined by measuring the absorbance at 254.1 and 309.1 nm (corresponding to zero difference of benzocaine), while the absorbance difference at 230.1 and 263.5 nm (corresponding to zero difference of antipyrine) was selected for benzocaine determination in the laboratory prepared spectrum. Method B depends on measuring the peak amplitude of first derivative at 305 nm for calculating benzocaine concentration then the total concentration of both drugs was determined using isoabsorptive point at 257.4 nm (antipyrine concentration was then calculated by subtraction). Method C is based on measuring the peak difference of the ratio spectra at Dp (239.1-285 nm) and Dp (301.4-250 nm) for the determination of antipyrine and benzocaine, respectively. Method D depends on measuring peak to peak amplitude of the first derivative of ratio spectra at (234.5 + 244.2 nm) and peak amplitude at 295.5 nm for the determination of antipyrine and benzocaine, respectively. The proposed methods were validated and applied for the analysis of antipyrine and benzocaine in their laboratory prepared mixtures and pharmaceutical formulation. Statistical comparison between the results of the proposed methods and those of the reported methods showed no significant difference. Ó 2016 Production and hosting by Elsevier B.V. on behalf of Faculty of Pharmacy, Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).

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Section: Resultsmentioning

confidence: 97%

Section: Accuracymentioning

confidence: 99%

“…Purity of AN and BE was found to be 100.22 ± 1.375 and 99.47 ± 1.071 according to its reported method 30. 2.3.2.…”

mentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine

Merey

2016

Bulletin of Faculty of Pharmacy, Cairo University

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Design of imprinting matrix for dual template sensing via electropolymerized polythiophene films

Singh

2022

J of Molecular Recognition

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This work presents the design of 3-thiophene acetic acid (3-TAA) polymer matrix based molecularly imprinted polymer (MIP)/reduced graphene oxide (RGO) composite for sensitive and selective detection of antipyrine (AnP) and ethionamide (ETH) simultaneously. Dual drug embedded molecularly imprinted polymer (MIP) based electrochemical sensor was developed via electropolymerization of 3-TAA. AnP and ETH were embedded inside a polymer matrix based on their 3-D orientation and interaction(s) with functional monomer(s). Their extraction from polymeric matrix generates cavities complimentary to shape and size of AnP and ETH. The extraction of templates was confirmed by differential pulse voltammetry (DPV) as well as highperformance liquid chromatography (HPLC). The designed sensor selectively captures and produces the electrochemical signal for imprinted drugs. The electrochemical behaviour of AnP and ETH was investigated by DPV technique. The sensitivity forboth drug molecules was commendable on a single polymeric composite with RGO on GC electrode (LOD of 0.117 μM for AnP and 0.15 μM for ETH). Also, the sensor exhibited excellent selectivity towards AnP and ETH in the presence of other analogous interferent molecules. Thus, the designed sensor showed high sensitivity as well as high selectivity for imprinted dual drug molecules on a single platform.

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A simple and fast method for determination of benzocaine and lidocaine in pharmaceutical formulations by capillary electrophoresis with spectrophotometric detection

Junger

Jesus

Silva

et al. 2019

Separation Science Plus

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A novel, simple, and rapid method for the determination of the anesthetics benzocaine and lidocaine in pharmaceutical formulations using capillary electrophoresis with spectrophotometric detection (210 nm) is proposed. In this method, the sample preparation was fast and simple, using only aqueous dilution or extraction with sonication aid. A background electrolyte composed by 10 mmol L ‐1 of monosodium phosphate with pH 2.1 allowed the separation of the anesthetics with good peak resolution in less than 5 min. The limits of quantification were 0.31 and 0.68 mg L−1 for lidocaine and benzocaine, respectively. The intra‐ and inter‐day precisions (RSD%) ranged from 1.1 to 4.8% and the accuracies (as recovery percentage) varied from 84 to 109.4%. The proposed method was successfully applied for analyses of commercial samples of pharmaceutical formulations, such as otologic solution, ointment, and spray solution and lozenges for sore throat.

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Validated simultaneous determination of antipyrine and benzocaine HCl in the presence of benzocaine HCl degradation product

Abstract: Two validated, sensitive and highly selective stability-indicating methods were adopted for the simultaneous quantitative determination of antipyrine (ANT) and benzocaine HCl (BEN) in the presence of the degradation product of benzocaine HCl [p-aminobenzoic acid (PABA)].

Cited by 11 publications

References 19 publications

Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine

Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine

Design of imprinting matrix for dual template sensing via electropolymerized polythiophene films

A simple and fast method for determination of benzocaine and lidocaine in pharmaceutical formulations by capillary electrophoresis with spectrophotometric detection

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