Validated spectrofluorimetric method for determination of selected aminoglycosides

Omar, Mahmoud A.; Ahmed, Hytham M.; Hammad, Mohamed A.; Derayea, Sayed M.

doi:10.1016/j.saa.2014.07.020

Cited by 45 publications

(16 citation statements)

References 31 publications

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“…The sample weight before soaking was measured as W 1 , the sample weight after soaking and cleaning was measured as W 2 , the weight loss: Δ W = ( W 1 − W 2 )/ W 2 . The concentration of tobramycin eluted was measured as described elsewhere . The fluorescent intensity was measured at 460 nm after excitation at 360 nm using a microplate reader (Synergy HT, BioTek Instruments, and Winooski, VT) and converted to concentration using a standard curve of known tobramycin concentrations.…”

Section: Methodsmentioning

confidence: 99%

“…The concentration of tobramycin eluted was measured as described elsewhere. 30 The fluorescent intensity was measured at 460 nm after excitation at 360 nm using a microplate reader (Synergy HT, BioTek Instruments, and Winooski, VT) and converted to concentration using a standard curve of known tobramycin concentrations. A similar method was used to measure the concentration of vancomycin eluted, taking advantage of vancomycin's ultraviolet properties.…”

Section: Antibiotics Release and Bactericidal Activity Assaymentioning

confidence: 99%

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Release of vancomycin and tobramycin from polymethylmethacrylate cements impregnated with calcium polyphosphate hydrogel

et al. 2017

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The influence of calcium polyphosphate (CPP) gel incorporation on the release of vancomycin and tobramycin from polymethyl methacrylate (PMMA) cement (Simplex P, SP) has been studied. Adding 10% CPP gel to SP led to a much lower burst release of vancomycin and considerably extended release of both vancomycin and tobramycin up to 24 weeks. Antibiotics released from this new material retain their bactericidal activity for up to 15 weeks. The improvement in the antibiotic release is mainly due to the molecular interactions of antibiotics with embedded CPP polyphosphate chains as confirmed by Raman spectroscopy analysis. The inclusion of CPP hydrogel also increased the SP surface roughness and pore sizes, leading to a higher release rate of antibiotics. The new material is biocompatible and has similar handling properties and mechanical strength as compared to SP cements. We believe that incorporating CPP gel provides a better and usable drug carrier for PMMA cement. © 2017 The Authors Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2827-2840, 2018.

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Section: Methodsmentioning

confidence: 99%

Section: Antibiotics Release and Bactericidal Activity Assaymentioning

confidence: 99%

Release of vancomycin and tobramycin from polymethylmethacrylate cements impregnated with calcium polyphosphate hydrogel

et al. 2017

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“…Concentration of amikacin in eluates was determined by reacting 10-fold diluted samples with a mixture of acetylacetone and formaldehyde in a buffer solution of boric acid, acetic acid, and phosphoric acid at pH 2.7 and boiling for 20 minutes. Fluorescence of the reacted product was measured at 471 nm (410 nm excitation) with a BioTek Synergy plate reader [46]. All concentrations were normalized to standard curves of known concentrations of antibiotics.…”

Section: Elution and Eluate Activity Studiesmentioning

confidence: 99%

Novel Antibiotic-loaded Point-of-care Implant Coating Inhibits Biofilm

Jennings

Carpenter

Troxel

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background Orthopaedic biomaterials are susceptible to biofilm formation. A novel lipid-based material has been developed that may be loaded with antibiotics and applied as an implant coating at point of care. However, this material has not been evaluated for antibiotic elution, biofilm inhibition, or in vivo efficacy.Questions/purposes (1) Do antibiotic-loaded coatings inhibit biofilm formation? (2) Is the coating effective in preventing biofilm in vivo? Methods Purified phosphatidylcholine was mixed with 25% amikacin or vancomycin or a combination of 12.5% of both. A 7-day elution study for coated titanium and stainless steel coupons was followed by turbidity and zone of inhibition assays against Staphylococcus aureus and Pseudomonas aeruginosa. Coupons were inoculated with bacteria and incubated 24 hours (N = 4 for each test group). Microscopic images of biofilm were obtained. After washing and vortexing, attached bacteria were counted. A mouse biofilm model was modified to include coated and uncoated stainless steel wires inserted into the lumens of catheters inoculated with a mixture of S aureus or P aeruginosa. Colony-forming unit counts (N = 10) and scanning electron microscopy imaging of implants were used to determine antimicrobial activity. Results Active antibiotics with colony inhibition effects were eluted for up to 6 days. Antibiotic-loaded coatings inhibited biofilm formation on in vitro coupons (log-fold reductions of 4.3 ± 0.4 in S aureus and 3.1 ± 0 for P aeruginosa in phosphatidylcholine-only coatings, 5.6 ± 0 for S aureus and 3.1 ± 0 for P aeruginosa for combinationloaded coatings, 5.5 ± 0.3 for S aureus in vancomycinloaded coatings, and 3.1 ± 0 for P aeruginosa for amikacinloaded coatings (p \ 0.001 for all comparisons of antibiotic-loaded coatings against uncoated controls for both bacterial strains, p \ 0.001 for comparison of antibioticloaded coatings against phosphatidylcholine only for S aureus, p = 0.54 for comparison of vancomycin versus combination coating in S aureus, P = 0.99 for comparison of antibiotic-and unloaded phosphatidylcholine coatings in P aeruginosa). Similarly, antibiotic-loaded coatings reduced attachment of bacteria to wires in vivo (log-fold

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“…The current study aimed to establish a simple, reliable, selective and cheap spectrofluorimetric method for analysis of the investigated drugs in their pure forms and in pharmaceutical formulations using Hantzsch dihydropyridine synthesis as a multicomponent organic reaction between an aldehyde (formaldehyde), two equivalents of a β‐keto ester (ethyl acetoacetate) and a nitrogen donor (studied drugs). The proposed method is highly sensitive (70–900 ng ml −1 ) and can be used to determine the studied drugs in human plasma.…”

Section: Introductionmentioning

confidence: 99%

Utility of Hantzsch reaction for development of highly sensitive spectrofluorimetric method for determination of alfuzosin and terazosin in bulk, dosage forms and human plasma

2017

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A highly sensitive, cheap, simple and accurate spectrofluorimetric method has been developed and validated for the determination of alfuzosin hydrochloride and terazosin hydrochloride in their pharmaceutical dosage forms and in human plasma. The developed method is based on the reaction of the primary amine moiety in the studied drugs with acetylacetone and formaldehyde according to the Hantzsch reaction, producing yellow fluorescent products that can be measured spectrofluorimetrically at 480 nm after excitation at 415 nm. Different experimental parameters affecting the development and stability of the reaction products were carefully studied and optimized. The fluorescence-concentration plots of alfuzosin and terazosin were rectilinear over a concentration range of 70-900 ng ml , with quantitation limits 27.1 and 32.2 ng ml for alfuzosin and terazosin, respectively. The proposed method was validated according to ICH guidelines and successfully applied to the analysis of the investigated drugs in dosage forms, content uniformity test and spiked human plasma with high accuracy.

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Validated spectrofluorimetric method for determination of selected aminoglycosides

Cited by 45 publications

References 31 publications

Release of vancomycin and tobramycin from polymethylmethacrylate cements impregnated with calcium polyphosphate hydrogel

Release of vancomycin and tobramycin from polymethylmethacrylate cements impregnated with calcium polyphosphate hydrogel

Novel Antibiotic-loaded Point-of-care Implant Coating Inhibits Biofilm

Utility of Hantzsch reaction for development of highly sensitive spectrofluorimetric method for determination of alfuzosin and terazosin in bulk, dosage forms and human plasma

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