Background: The coronavirus diseases (COVID-19) pandemic has been substantially affecting the life of people worldwide, especially when World Health Organization declared it as a global pandemic in the second week of March 2020. It produced momentous anguish throughout the biosphere. Apart from the increased in COVID infested reports, it instigated a considerable disturbance to psychological healthiness in the affected nations. Many nation states across the world, had executed a countrywide constrainment on all human activities and jobs to control the spread of the virus. The present study is an attempt to find out psychological distress among people residing in Pakistan during the lockdown.Methods: Four hundred and forty three participants that were inhabitants of Karachi, Pakistan were asked to complete questionnaire. It was conducted online to maintain quarantine effect including questions about symptoms of depression, anxiety, stress, and family affluence according to Depression anxiety and stress scale 21 (DAAS-21 scale). Selection of participants was done by consecutive sampling method.Results: The results indicated that people who were economically weak and unstable to endure the lockdown were generally affected. While awareness and fear of getting COVID-19 affluence was found to be negatively correlated with stress, anxiety, and depression. Among gender females were experiencing stress, anxiety, and depression more than males and the most affected age group were adults. Conclusion: Moderate depression, severe anxiety and lower level of stress was seen in the population of Karachi during sudden first lockdown due to spread of COVID-19. Government officials and other establishments may take the support and corporation of professionals related to psychiatry and psychotherapy to assist in overwhelming the psychosocial problems among the homeland related to COVID-19 lockdown.