Validation and additional support for an experimental animal model of fibromyalgia

Abstract

Our results show that ICS is a useful animal model to assess hypothesis about underlying mechanisms involved in the development of fibromyalgia as well as to evaluate possible future therapies.

Unsaponifiable fraction isolated from grape (Vitis vinifera L.) seed oil attenuates oxidative and inflammatory responses in human primary monocytes

“…The UF was isolated from GSO (1 Kg, Naturgreen, Murcia, Spain) following conventional procedures and its components were analysed following the IUPAC method and described in Montserrat-de la Paz et al 7 In brief, GSO was saponified at 80ºC by refluxing with 50 ml of 2 N potassium hydroxide solution in ethanol (Panreac, Barcelona, Spain), boiling gently until the solution became clear and then for additional 20 min. Heating was stopped by addition of 50 ml distillate water through the top of the condenser and the solution was Table 1).…”

“…MTT-formazan crystals were solubilized with DMSO (Sigma), and then measured with a microplate reader at 570 nm corrected to 650 nm. 15 Cell survival was expressed as the percentage of absorbance compared with that obtained in control, non-treated cells.…”

“…The UF, about 1.5-2% of the oils, is an important source of interesting minor compounds. 7,8 As part of ongoing investigations on bioactive secondary plant metabolites in medicinal and food plants, our aim of the present study was to conduct a detailed analysis to establish the anti-oxidant and anti-inflammatory effects for this valuable oilseed crop.…”

Antinociceptive effect of Valeriana fauriei regulates BDNF signaling in an animal model of fibromyalgia

“…Fibromyalgia (FM) is characterized by generalized tenderness in ≥11 of 18 tender points (1) and chronic widespread pain that lasts >3 months (2). Mechanical hyperalgesia is a common symptom of FM (3), which is a painful syndrome of largely unknown etiology and pathology that is often accompanied by various phenomena. In addition, emerging evidence has indicated that pain amplification within the central nervous system serves an important role in the pathology of FM-associated pain (4), which is associated with numerous other symptoms (5).…”

“…The symptoms of this painful syndrome include fatigue (6), anxiety (7), sleep disturbance (8), temporomandibular disease and depression (9). An animal model of FM must include widespread pain and associated symptoms of fatigue and psychological disturbance (3).…”