Unsaponifiable fraction isolated from grape (Vitis vinifera L.) seed oil attenuates oxidative and inflammatory responses in human primary monocytes

Abstract

Grape (Vitis vinifera L.) seed has well-known potential for production of oil as a byproduct of winemaking and is a rich source of bioactive compounds. Herein, we report that the unsaponifiable fraction (UF) isolated from grape seed oil (GSO) possesses anti-oxidative and anti-inflammatory properties towards human primary monocytes. The UF isolated from GSO was phytochemically characterized by GC-MS and HPLC. Freshly obtained human monocytes were used to analyse the effects of GSOUF (10-100 μg mL-1) on oxidative and inflammatory responses using FACS analysis, RT-qPCR, and ELISA procedures. GSOUF skewed the monocyte plasticity towards the anti-inflammatory non-classical CD14+CD16++ monocytes and reduced the inflammatory competence of LPS-treated human primary monocytes diminishing TNF-α, IL-1β, and IL-6 gene expression and secretion. In addition, GSOUF showed a strong reactive oxygen species (ROS)-scavenging activity, reducing significantly nitrite levels with a significant decrease in Nos2 gene expression. Our results suggest that the UF isolated from GSO has significant potential for the management of inflammatory and oxidative conditions and offer novel benefits derived from the consumption of GSO in the prevention of inflammation-related diseases.

Does unsaponifiable fraction of grape seed oil attenuate nitric oxide production, oxidant and cytotoxicity activities

“…Furthermore, the inhibition efficiency of UF at 10 μg/ml was weaker than the other concentrations (25 and 50 μg/ml) which did not exceed 28.64% in Carignan, 8.37% in Merlot and 4.10% in Syrah. Interestingly, our data were in accordance with previously published work showing that the UF of grape seed oil inhibited nitric oxide production in human primary monocytes (Millan‐Linares et al, ). So, the potent inhibitory activity of grape seed oil could be attributed, at least partly, to its UF containing several minor components (sterols, tocopherols, and triterpene alcohols).…”

Grape (Vitis vinifera L.) Seed Oil: A Functional Food from the Winemaking Industry

“…Both antioxidant and anti-inflammatory activities of grape seed oil have been reported as the basis of another neuroprotective and hepatoprotective behavior of grape seed oil tested in animals [ 136 , 137 ]. In human cells, grape seed oil hydrophilic components have already been shown to attenuate oxidative and inflammation conditions [ 138 ].…”

GPETAFLR, an octapeptide isolated from Lupinus angustifolius L. protein hydrolysate, promotes the skewing to the M2 phenotype in human primary monocytes

“…7 Therefore, the use of primary monocytes as a model of inflammation is widespread and is used in numerous studies to test the anti-inflammatory activity of various compounds. 8,9 The secretion of pro-inflammatory mediators works as an inflammatory beacon for leukocytes, which contribute to all stages of several inflammatory disorders, therefore representing an important therapeutic target. 10 Human monocytes are classified into three subsets: CD14 ++ CD16 -(classical monocytes), intermediate CD14 ++ CD16 + (intermediate monocytes), and CD14 + CD16 ++ (non-classical monocytes).…”