Validation Assay for Total Flavonoids, as Rutin Equivalents, from Trichilia catigua Adr. Juss (Meliaceae) and Ptychopetalum olacoides Bentham (Olacaceae) Commercial Extract

Rolim, André; Maciel, Carolina Penteado Moraes; Kaneko, Telma Mary; Consiglieri, Vladi Olga; Salgado-Santos, Idalina Maria Nunes; Velasco, Maria Valéria Robles

doi:10.1093/jaoac/88.4.1015

Cited by 65 publications

(33 citation statements)

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“…Aluminum nitrate-sodium nitrite colorimetric method was used to determine the TF concentration of samples, using rutin (Sigma-Aldrich Co.) as a standard (Rolim et al 2005). The standard solution (150 mg/mL with methanol) 0, 0.50, 1.00, 2.00, 3.00, 4.00 mL were moved to a tube and the volume made up to 5 mL, and then mixed with 0.3 mL sodium nitrite solution (5%, w/v), respectively.…”

Section: Determination Of Tp Tf Tt Content and Antioxidant Activitymentioning

confidence: 99%

Influence of Harvest Season and Drying Method on the Antioxidant Activity and Active Compounds of Two Bamboo Grass Leaves

Zhang

et al. 2013

Journal of Food Processing and Preservation

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Changes of the antioxidant activity and active compounds content from two bamboo grass leaves, namely, Pleioblastus kongosanensis f. aureostriatus and Shibataea chinensis, as affected by different harvest season and drying methods were investigated and the main compounds that influence the antioxidant activity of two bamboo grasses were analyzed by principal component analysis. The content of total flavonoids, phenolics, triterpenoids and eight active compounds, i.e., orientin, isoorientin, vitexin, homovitexin and p‐coumaric acid, chlorogenic acid, caffeic acid, ferulic acid were determined by UV‐spectrophotometer and reverse phase high performance liquid chromatography synchronously, respectively, while antioxidant activity was measured using diphenyl‐2‐picryl‐ hydrazyl and ferric reducing antioxidant power methods. Both bamboo grasses leaves showed the highest level of total active components and antioxidant activity in winter. Different drying methods also affected the active compounds. Lyophilization, followed by vacuum drying and microwave oven drying, was the most suitable method for keeping active components, yet considering the energy consuming, microwave oven drying was recommended for bamboo grass leaves. Practical Applications Bamboo leaves, with excellent free radical scavenging, antioxidation, antibacterial, antiviral, enhancing immunity and other biological effectiveness, have been used for over 1,000 years in Asia as health food material and folk medicine. Bamboo grasses leaves, with advantages of rapid growth, lush foliage and easy collection, were a kind of new potential leafy bamboo grove. In order to improve the comprehensive utilization of leaves and exploit them more scientifically and efficiently, the present study investigated the changes of total phenolics, flavonoids, triterpenoid and eight active compounds content as well as antioxidant actives in leaves in accordance with seasonal variation and different drying methods. The results showed the optimum season for bamboo grass leaves harvest is at winter, and microwave oven drying was finally suggested for postharvest handling. It may provide a theoretical basis for further suitable exploitation and utilization of the leaf resources of the bamboo grasses.

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Section: Determination Of Tp Tf Tt Content and Antioxidant Activitymentioning

confidence: 99%

Influence of Harvest Season and Drying Method on the Antioxidant Activity and Active Compounds of Two Bamboo Grass Leaves

Zhang

et al. 2013

Journal of Food Processing and Preservation

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“…Total Flavonoid Quantification. Total flavonoid content (TFc) was determined following the method by Rolim et al (2005) with modifications. One hundred milligrams (dry basis) of extracts was dissolved in 5 mL of water in a volumetric flask.…”

Section: Determination Of the Properties Of The Dried Extractsmentioning

confidence: 99%

Optimization of the Spray‐Drying Process for Developing Jabuticaba Waste Powder Employing Response Surface Methodology

Borges

Martins

Conceição

et al. 2015

J Food Process Engineering

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The objective of this work was to obtain spray-dried extract from seeds and peels jabuticaba (Myrciaria cauliflora) with optimized properties. To optimize the spray-drying process, a 3 3 Box-Behnken design was used, in which the independent variables were the inlet drying air temperature (x1, 100-120C), feed flow rate (x2, 3-5 mL/min) and spray nozzle air flow rate (x3, 40-50 L/min). The dependent variables were ellagic acid content, total flavonoid content, total phenolic content, antioxidant activity, water activity and moisture content. Only flavonoid content and moisture content were influenced by none of the independent variables in the ranges studied. Using the desirability function, the optimum conditions for obtaining spray-dried extracts for the responses (with significant effects) were inlet drying air temperature of 120C, feed flow rate of 3 mL/min and spray nozzle air flow rate of 50 L/min. Under these conditions, the results from the verification were 0.0844 ± 0.0013% for ellagic acid content, 16.27 ± 0.63 % for total phenolic content, 0.176 ± 0.02 for water activity and 54.45 ± 0.92% for antioxidant activity, which were in good agreement with the predicted values. PRACTICAL APPLICATIONSThis paper gives a report on the reuse of the vegetal wastes obtained from jabuticaba fruits -Myrciaria cauliflora (peels and seeds), which can be considered a raw material with several chemical compounds with biological activities. So, this work aims to arrive at the possible industrial application in the spraydrying process to obtain an intermediary product from jabuticaba wastes. Furthermore, surface response methodology (SRM) was employed to determine the influence of the most important spray dryer parameters on the physicochemical properties of M. cauliflora spray-dried extracts. Based on the results obtained from SRM and in biological activities known for this species, the spray-dried extracts exhibited a great potential for use in pharmaceutical and nutraceutical fields.

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“…The polyphenols contents was determined using the method described by Sadasivam and Manickam (2008). The flavonoids were extracted and subjected to column chromatograph according to Rolim et al, (2005) and Kujala et al, (2000).…”

Section: Phytochemical Analysis Quantitative Estimation Of Polyphenolmentioning

confidence: 99%

AUTECOLOGY AND METABOLIC PRODUCTS OF Calotropis procera (Aiton) W. T. Aiton.

Ziada

Al-Shami

Kraeeshi

2015

Journal of Plant Production

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The present study was conducted to evaluate the ecological characteristics and the bioactive metabolic products of C. procera. Also, the crude methanol extract of the aerial parts of calotropis was examined for antioxidant and antimicrobial potentialilties. The scrubland dominated by C. procera represented an advanced stage of desert vegetation and comprised 33 species related to 14 families. Tamarix nilotica, Alhagi graecorum, Launea mucronata, Trichodesma africanum and Hyoscyamus muticus were the common associates. The major life-forms were therophytes, geophytes and chamaephytes. The assemblage of this community belongs to eleven floristics categories with predominance of Sahara-Sindian, Sahara-Arabian and Sudano-Zambezian. The flavonoids and polyphenols contents of the aerial parts of C. procera were 0.185 and 0.37 g/100g, respectively. Thirteen fatty acids were detected among them undecanoic, palmitic, lauric, myristic and linoleic acids. The Calotropis methanolic extract exhibited free radical scavenging activity with IC50 value of 0.35 mg ml-1. It is showed antimicrobial spectrum only against Bacillus subtilis.

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Validation Assay for Total Flavonoids, as Rutin Equivalents, from Trichilia catigua Adr. Juss (Meliaceae) and Ptychopetalum olacoides Bentham (Olacaceae) Commercial Extract

Cited by 65 publications

References 0 publications

Influence of Harvest Season and Drying Method on the Antioxidant Activity and Active Compounds of Two Bamboo Grass Leaves

Influence of Harvest Season and Drying Method on the Antioxidant Activity and Active Compounds of Two Bamboo Grass Leaves

Optimization of the Spray‐Drying Process for Developing Jabuticaba Waste Powder Employing Response Surface Methodology

AUTECOLOGY AND METABOLIC PRODUCTS OF Calotropis procera (Aiton) W. T. Aiton.

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