Development of topical dosage forms requires physical

An ultraviolet spectrophotometric method was validated for total flavonoid quantitation, as rutin equivalents, present in the Trichilia catigua Adr. Juss (Meliaceae) and Ptychopetalum olacoides Bentham (Olacaceae) commercial extract. Parameters as linearity, interval (range), specificity, estimated limit of detection (LOD, μg/mL), estimated limit of quantitation (LOQ, μg/mL), recovery (R, %), precision or relative standard deviation (RSD, %), and accuracy (E, %) were established. The analytical method was validated according to the experimental results: correlation coefficient (r = 0.9997); interval (RSD = 0.15–0.47%; E = 98.98–101.24%); specificity to total flavonoids quantitation, as rutin equivalents, at wavelength 361.0 nm; LOD = 0.09 μg/mL and LOQ = 0.27 μg/mL; R = 99.36–102.14%; adequate intraand interrun precision (0.30–0.49% and 0.31–0.81%), and intra- and interrun accuracy (100.60–102.38% and 98.58–100.38%).

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A precise, accurate, and sensitive UV spectrophotometric method was developed and validated for routine quantification of total bioflavonoids, expressed as rutin, from a topical oil-in-water pharmaceutical emulsion containing the extract of Trichilia catigua Adr. Juss and Ptychopetalum olacoides Bentham. The method was validated experimentally, and the data were treated rigorously by statistical analysis. The following analytical parameters were assessed: linearity, specificity, intra- and interrun precision measured as relative standard deviation (RSD, %), intra- and interrun accuracy (E, %), recovery (Rec., %), limit of detection (LOD, μg/mL), and limit of quantification (LOQ, μg/mL). The UV spectrophotometric method was linear (r2 0.9995) for standard rutin over the concentration range of 5.015.0 g/mL with specificity for total bioflavonoids (expressed as rutin) at 361.0 nm with an absence of interferents from the complex matrix; RSD of ≤1.79%, intrarun (E 97.88 ±1.75 to 99.0 ± 0.33%) and interrun (E 98.38 ± 1.12 to 100.79 ± 1.30%) accuracy; Rec. 98.64 ± 0.42 to 100.74 ± 0.41%; LOD 0.20 μg/mL; and LOQ 0.30 μg/mL.