Validation of a Liquid Chromatography Method for the Simultaneous Determination of Several Nonsteroidal Anti-Inflammatory Drugs in Human Plasma for Therapeutic Drug Monitoring

Helmy, Sally A.; El-Bedaiwy, Heba M.

doi:10.1097/ftd.0b013e31829dcbed

Cited by 11 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As mentioned above, BSP was rapidly and completely converted to BOH in vivo so that BSP was rarely determined for the quantication in human plasma in vitro and in vivo. [13][14][15] In most studies, the pharmacokinetic processes of BSP and BDP were reected by their main metabolites BOH and B17P due to the low blood concentration and poor receptor-binding affinity at the glucocorticoid receptor of B21P compared with B17P. [16][17][18] In this study, the analytical method was accordingly validated to quantify BSP and BDP as well as their metabolites BOH, B17P and B21P in human plasma for a bioequivalence study.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of betamethasone sodium phosphate and betamethasone dipropionate in human plasma by UPLC-MS/MS and a bioequivalence study

et al. 2016

View full text Add to dashboard Cite

The compound medicine of betamethasone sodium phosphate (BSP) and betamethasone dipropionate (BDP) is widely used for diverse glucocorticoid-sensitive acute and chronic diseases such as asthma, rheumatoid arthritis and systemic lupus erythematosus. It will be useful and beneficial to validate sensitive method for the determination of BSP, BDP and their metabolites for their pharmacokinetic study. Hereby, an ultra-high pressure liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been validated for the determination of BSP, BDP and their metabolites betamethasone (BOH), betamethasone 17-monodipropionate (B17P) and betamethasone 21-monodipropionate (B21P) in human plasma. Liquid-liquid extraction with ether and n-hexane (v/v, 4:1) was used for sample preparation of BDP, BOH, B17P and B21P with beclomethasone dipropionate as internal standard (IS), while solid phase extraction was adopted for sample preparation of BSP using prednisolone as IS. The chromatographic separation was performed on a Hypurity C18 column (150 mm×2.1 mm, 5 μm) for BOH, BDP, B21P and B17P, and a Luna C18 (2) column (150 mm×2.0 mm, 5 μm) for BSP. Electrospray ionization interfaced with positive multiple reaction monitoring (MRM) scan mode was used for mass spectrometric detection. The standard calibration curves were linear within the range of 2.525 × 10−9−403.9 × 10−9 mol·dm−3 for BSP, 0.125 × 10−9−55.81 × 10−9 mol·dm−3 for BDP, 0.278 × 10−9−74.95 × 10−9 mol·dm−3 for BOH, 0.098 × 10−9−4.688 × 10−9 mol·dm−3 for B17P and 0.226 × 10−9−5.411 × 10−9 mol·dm−3 for B21P, respectively. The validated method was successfully applied to a bioequivalence study in 23 healthy subjects after they were injected with this compound medicine BSP and BDP.

show abstract

Section: Introductionmentioning

confidence: 99%

Quantitative determination of betamethasone sodium phosphate and betamethasone dipropionate in human plasma by UPLC-MS/MS and a bioequivalence study

et al. 2016

View full text Add to dashboard Cite

show abstract

“…A method based on solid‐phase membrane microtip extraction followed by HPLC has been developed and validated for the analysis of ibuprofen, ketoprofen and flurbiprofen from human plasma (Hussain, Al‐Ajmi, Samira Amir, & Ali, ). Simultaneous analysis of NSAIDs has been the subject of several reports in plasma and urine using protein precipitation (Helmy & El‐Bedaiwy, ), microextraction by packed sorbent (Locatelli et al, ) and graphene/Fe 3 O 4 ‐based dispersive magnetic solid‐phase extraction (Ferrone et al, ). Nevertheless, few methods have been reported to determine fenoprofen either alone (Poggi et al, ; Shibukawa, Nagao, Terakita, He, & Nakagawa, ) or in the presence of other NSAIDS (Maurer, Tauvel, & Kraemer, ; Nemoto et al, ; Suenami et al, ) in different biological matrices.…”

Section: Introductionmentioning

confidence: 99%

Quantification of fenoprofen in human plasma using UHPLC–tandem mass spectrometry for pharmacokinetic study in healthy subjects

Bharwad

Shah

Shrivastav

et al. 2019

Biomedical Chromatography

View full text Add to dashboard Cite

A rapid, simple and sensitive ultra‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method has been developed to quantify fenoprofen, a nonsteroidal anti‐inflammatory drug in human plasma for a pharmacokinetic study in healthy subjects. Owing to high levels of protein binding, protein precipitation followed by solid‐phase extraction was employed for the extraction of fenoprofen and fenoprofen‐d3 (used as internal standard) from 200 μL human plasma. Separation was performed on a BEH C18 (50 × 2.1 mm, 1.7 μm) column using methanol−0.2% acetic acid in water (75:25, v/v) under isocratic elution. Electrospray ionization was operated in the negative mode for sample ionization. Ion transitions used for quantification in the selected reaction monitoring mode were m/z 241/197 and m/z 244/200 for fenoprofen and fenoprofen‐d3, respectively. Under the optimized conditions, fenoprofen showed excellent linearity in the concentration range 0.02–20 μg/mL (r2 ≥ 0.9996), adequate sensitivity, favorable accuracy (96.4–103.7%) and precision (percentage coefficient of variation ≤4.3) with negligible matrix effect. The validated method was successfully applied to a pharmacokinetic study of fenoprofen in healthy subjects. The significant features of the method include higher sensitivity, small plasma volume for processing and a short analysis time.

show abstract

Separation and on‐line preconcentration of nonsteroidal anti‐inflammatory drugs by microemulsion electrokinetic chromatography

2015

View full text Add to dashboard Cite

The MEEKC separation and on-line preconcentration of nonsteroidal anti-inflammatory drugs (NSAIDs) were investigated. In this work, two microemulsion (ME) systems were employed. Complete separation could be achieved at a pH of 5.5 with octane as the oil phase (ME I). The LODs for the determination of flurbiprofen, fenoprofen, naproxen, ketoprofen, suprofen, and indoprofen were 0.45, 0.32, 0.10, 0.48, 0.77, and 0.29 mg/L, respectively. To enhance the sensitivity, anion selective exhaustive injection-sweeping (ASEI-sweeping) and stacking with reverse migrating pseudostationary phase (SRMP) were compared. For having a simple process without polarity switching and shorter analysis time, a suppressed EOF and di-n-butyl-L-tartrate as the oil phase (ME II) were used for the simultaneous determination of NSAIDs. The sensitivity for ASEI-sweeping was enhanced approximately 280-fold. However, there was much background noise, and some of the analytes were co-eluted. When SRMP was used, all of the analytes could be detected simultaneously, and baseline separation could be achieved. The sensitivities of all of the analytes, except for indoprofen, were enhanced approximately 170-fold compared with the normal MEEKC, and the LODs were 2-45 μg/L. Herein, we propose a simple method for the direct determination of NSAIDs at therapeutic and toxic levels in urine throughout a wide concentration range.

show abstract

Validation of a Liquid Chromatography Method for the Simultaneous Determination of Several Nonsteroidal Anti-Inflammatory Drugs in Human Plasma for Therapeutic Drug Monitoring

Cited by 11 publications

References 20 publications

Quantitative determination of betamethasone sodium phosphate and betamethasone dipropionate in human plasma by UPLC-MS/MS and a bioequivalence study

Quantitative determination of betamethasone sodium phosphate and betamethasone dipropionate in human plasma by UPLC-MS/MS and a bioequivalence study

Quantification of fenoprofen in human plasma using UHPLC–tandem mass spectrometry for pharmacokinetic study in healthy subjects

Separation and on‐line preconcentration of nonsteroidal anti‐inflammatory drugs by microemulsion electrokinetic chromatography

Contact Info

Product

Resources

About