Validation of a liquid chromatography with ultraviolet detection methodology for the determination of sulfonamides in bovine milk according to 2002/657/EC

Granja, Rodrigo H M M; Lima, Andreia C de; Salerno, Alessandro G.; Wanschel, Amarylis

doi:10.1016/j.foodcont.2012.05.018

Cited by 19 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These well‐known drugs are administered to livestock as therapy, disease prolepsis, and growth promotion. The prevalent use of these antibiotic drugs in livestock leads to their presence as residues at trace and ultra‐trace level concentrations in dairy products and in milk .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

One‐pot synthesis of a multi‐template molecularly imprinted polymer for the extraction of six sulfonamide residues from milk before high‐performance liquid chromatography with diode array detection

Kechagia

Samanidou

Kabir

et al. 2017

J of Separation Science

View full text Add to dashboard Cite

A highly selective molecularly imprinted polymer sorbent was synthesized and employed for the simultaneous determination of six sulfonamide antibiotic residues (sulfanilamide, sulfacetamide, sulfadiazine, sulfathiazole, sulfamerazine, and sulfamethizole) in milk samples. Multi-analyte imprinted particles were used as a sorbent in solid-phase extraction. Sulfonamides were separated on a high-performance liquid chromatography column (Merck-Lichrospher RP18e, 5 μm 250 × 4 mm) and further identified and quantified by diode array detection. Several parameters including required loading of the molecularly imprinted polymer sorbent, mass of milk, volume, and type of elution solvent, as well as time for absorption and elution were investigated to obtain optimal experimental conditions. For comparison purpose, a non-imprinted polymer was applied under the optimum conditions. The validation study according to the European Union Decision 2002/657/EC was based on the investigation of linearity, selectivity, stability, limits of detection and quantitation, decision limit, detection capability, trueness, precision, and ruggedness according to Youden's approach. The decision limit and detection capability values in the milk were achieved from 101.9 to 113.5 μg/kg and from 114.4 to 135.4 μg/kg, respectively, depending on the target sulfonamide drug. Finally, the optimized protocol was successfully applied to commercial milk samples and human breast milk.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Milk is a liquid consisting of water, lactose, protein, fat, minerals, and vitamins. Due to the high nutritional benefit, it is one of the most consumed foods . The presence of antibiotic drug residues in milk samples could put the consumer's health in danger.…”

Section: Introductionmentioning

confidence: 99%

One‐pot synthesis of a multi‐template molecularly imprinted polymer for the extraction of six sulfonamide residues from milk before high‐performance liquid chromatography with diode array detection

Kechagia

Samanidou

Kabir

et al. 2017

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Deproteinization helps to prevent the emulsion formation during sample treatment; additionally, the obtained aqueous matrix (acidic pH value) is suitable for extraction of the SMX by DSPE. [27,28] Subsequently, 1.0 mL of the liquid phase was taken, adjusted the pH value at 2.0 (adding H 3 PO 4 1.0 M), and diluted to 10 mL with deionized water in a calibration flask (solution A).…”

Section: Sample Analysismentioning

confidence: 99%

Dispersive solid-phase extraction based on butylamide silica for the determination of sulfamethoxazole in milk samples by capillary electrophoresis

Islas

Rodríguez

Páez-Hernández

et al. 2016

Journal of Liquid Chromatography & Related Technologies

View full text Add to dashboard Cite

A new method based on the combination of dispersive solid-phase extraction and capillary electrophoresis is proposed for the determination of sulfamethoxazole in milk samples. Butylamide silica was synthesized and used as extractant. Factors involved in sample treatment method such as: butylamide silica amount, NaOH concentration in methanol, sample volume, and dispersion time were evaluated using a Taguchi parameter design. Under optimal conditions, average recoveries ranged from 73 to 85% with a limit of detection of 0.05 mg L À 1 were achieved. The proposed method is a useful technique for cleanup milk samples. GRAPHICAL ABSTRACT

show abstract

“…Conventionally, numerous screening techniques for the determination of SAs residues in animal tissues such as LC-MS/MS, [38][39][40] HPLC 13,[41][42][43][44][45] coupled with fluorescence (FL), 41 ultraviolet (UV), 46,47 or diode array detector (DAD), [48][49][50] capillary electrophoresis with UV-detector 51 or tandem mass spectrometry (CE-MS/MS), 52 fluorescence spectrometry and cyclic voltammetry, 53 and flow-through immunoaffinity chromatography 54 have been employed. Among these techniques, derivatization with fluorescamine has been receiving much attention as it can separate and detect various SAs and enhance the screening sensitivity of the procedure.…”

Section: Introductionmentioning

confidence: 99%

Development of an Environmentally Friendly Extraction Method Using Smartphone-Based Digital Images for the Determination of Total Sulfonamides in Meat Samples

Maroubo¹,

Melchert²

2023

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Sulfonamides (SAs) are low-cost synthetic antimicrobials widely used in veterinary and human medicine to treat diseases and prevent infections. However, long periods of exposure to sulfonamides can cause adverse effects such as allergic reactions. This work aims to study dispersive solid-liquid microextraction as an alternative method for extracting total SAs in meat samples. The procedure uses a colorimetric reaction based on the formation of a pink compound (imine salt) to determine total sulfonamides (sulfamethazine, sulfadimethoxine, sulfathiazole) with digital measurements. A linear response was observed between 33-233 μg kg-1 for total sulfonamides, and the coefficient of variation (n = 11; 67 μg kg-1 of total SAs) and the limit of detection were estimated to be 0.63% and 10 μg kg-1, respectively. For a 750 mg meat sample, 0.11 mg of 4-dimethylaminocinnamaldehyde, 2.60 mg of sodium dodecyl sulfate, and 275 μL of 1-butanol were consumed per sample, and consequently, generating only 335 μL of residue. Besides this, addition-recovery tests were performed, resulting in a 71-100% recovery range, indicating the trueness of the proposed method.

show abstract

Validation of a liquid chromatography with ultraviolet detection methodology for the determination of sulfonamides in bovine milk according to 2002/657/EC

Cited by 19 publications

References 13 publications

One‐pot synthesis of a multi‐template molecularly imprinted polymer for the extraction of six sulfonamide residues from milk before high‐performance liquid chromatography with diode array detection

One‐pot synthesis of a multi‐template molecularly imprinted polymer for the extraction of six sulfonamide residues from milk before high‐performance liquid chromatography with diode array detection

Dispersive solid-phase extraction based on butylamide silica for the determination of sulfamethoxazole in milk samples by capillary electrophoresis

Development of an Environmentally Friendly Extraction Method Using Smartphone-Based Digital Images for the Determination of Total Sulfonamides in Meat Samples

Contact Info

Product

Resources

About