Validation of a liquid chromatography tandem mass spectrometry assay for serum creatinine and comparison with enzymatic and Jaffe methods

Abstract

Background Glomerular filtration rate (GFR) can be estimated using creatinine clearance or calculated formulae, methods which rely on creatinine quantification in serum. Creatinine is most frequently measured using the Jaffe method, but this is prone to interference by, for example, bilirubin, protein and ketones. Recent amendments to this assay have been made by manufacturers in an attempt to compensate for the protein interference.

Dietary intake according to hydration status in 9-10 year-old soccer players

“…The urine collection was stored in individual containers that were maintained at low temperatures until analyzed. A certified laboratory analyzed the urine samples to determine urine volume (ml), creatinine (mg/d) and sodium content (mg/d) (12). Every child had a weight-related 24 h creatinine excretion rate equal or higher than 0.1 moll kg -1 d -1 (13,14), so the total sample was considered.…”

Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography–tandem mass spectrometry: Application in basal and stimulated conditions

“…The plate was placed in a Acquity UPLC system (Waters, Manchester, UK) and 40 L of extract was injected onto a 4 mm × 2 mm C8 Gemini guard cartridge which was attached to a 30 mm × 3 mm 4 m Synergy Hydro-RP C18 analytical column (both Phenomenex, Macclesfield, UK), for on-line solid phase extraction as described previously [11,12]. The mobile phases were: A = water with 2 mmol/L of ammonium acetate and 0.1% (v/v) formic acid and B = methanol with 2 mmol/L of ammonium acetate and 0.1% (v/v) formic acid.…”

Renal Dysfunction and Liver Transplantation

“…Serum creatinine is usually measured by the Jaffè method, but this is prone to interference, for example, from protein, ketones and bilirubin. Hence, hyperbilirubinemia often impacts on the measurement of Scr in endstage liver disease population (Owen et al, 2006). These findings can result in an underestimation of renal function.…”