Validation of a Monoclonal Stool Antigen Test for Diagnosing <i>Helicobacter pylori</i> Infection in Young Children

Raguza, Daniele; Machado, Rodrigo Strehl; Ogata, Silvio Kazuo; Granato, Celso Francisco Hernandes; Patricio, Francy R.; Kawakami, Elisabete

doi:10.1097/mpg.0b013e3181ac6770

Cited by 39 publications

(32 citation statements)

References 37 publications

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“…The results of this imunocromatographic test are in disagreement with the immunoassay tests executed in Brazil (2,3,11,13,14,(16)(17)(18)21). Our results are also in disagreement with some results obtained even in different areas of the world, by immunocromatographic assay (4,9,18) .…”

Section: Discussioncontrasting

confidence: 56%

“…Due to these characteristics, the use of FAT by immunochromatography in developing countries is attractive. To the best of our knowledge, only nine studies (2,3,11,13,14,(16)(17)(18)21) in the past ten years evaluated FAT to diagnosis H. pylori infection in Brazil, most of them used immuno-enzymatic assay and only one used immunochromatography (18) .…”

Section: Introductionmentioning

confidence: 99%

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Is the Immunocromatographic Fecal Antigen Test Effective for Primary Diagnosis of Helicobacter Pylori Infection in Dyspeptic Patients?

Nora

Hörner

Carli

et al. 2016

Arq. Gastroenterol.

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-Background -The diagnosis of H. pylori infection can be performed by non-invasive and invasive methods.The identification through a fecal antigen test is a non-invasive, simple, and relatively inexpensive test. ) and negative predictive value 73,2% (CI95% 67.5-77.6); Positive likelihood ratio was 4.7 (CI95% 2.9-7.9) and Negative Likelihood Ratio 0.4 (CI95% 0.3-0.5). The prevalence odds ratio for a positive test was 12.3 (CI95% 5.7-26.3). The index kappa between FAT and histology/urease test was 0.53 (CI95% 0.39-0.64). Conclusion -Immunochromatographic FAT is less expensive than the other methods and readily accepted by the patients but its diagnostic performance does not recommend its use in the primary diagnosis, when the patient may have an active infection.

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Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Is the Immunocromatographic Fecal Antigen Test Effective for Primary Diagnosis of Helicobacter Pylori Infection in Dyspeptic Patients?

Nora

Hörner

Carli

et al. 2016

Arq. Gastroenterol.

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“…A escolha de um teste não invasivo para o estudo epidemiológico em crianças é de fundamental importância. Dois testes não invasivos validados localmente estavam disponíveis: o teste respiratório da ureia-13 C e o teste do antígeno fecal utilizando anticorpos monoclonais 14 . Em nosso estudo de validação local, a sensibilidade foi de 93,3% (IC95% 86,9%-99,8%), e a especificidade foi de 96,2% (IC95% 93,6-98,8%) 13 .…”

Section: Discussionunclassified

Prevalence of Helicobacter pylori infection and intestinal parasitosis in children of the Xingu Indian Reservation

Escobar-Pardo¹,

Godoy²,

Machado³

et al. 2011

J Pediatr (Rio J)

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Objective: To evaluate the prevalence of Helicobacter pylori infection and its association with intestinal parasitoses in children from indigenous communities of the Xingu Indian Reservation, in Brazil.Methods: A total of 245 Native Brazilian children between 2 and 9 years of age, from six villages of the Xingu River region, a tributary of the Amazon River, were assessed. H. pylori was detected using the 13 C-urea breath test. Breath samples were collected at baseline and 30 minutes after ingestion of 50 mg of 13 C-urea diluted with 100 mL of water flavored with passion fruit juice and sweetener. Stool samples were collected for the stool ova and parasites exam for 202/245 (82.4%) children. Results:The overall prevalence of H. pylori was 73.5%. A significant association of H. pylori with increased age was observed among the different villages and ethnic groups. Positive results for the presence of parasites -97.5% (198/202) -from the stool samples collected showed no association with H. pylori. Giardia showed an association with H. pylori in the multivariate analysis. Risk factors for H. pylori infection were observed in Kisêjê and Kaibi ethnic groups (OR [odds ratio] = 3.36 and 4.00, respectively), as well as in Tuiararé, Ngojwere, Capivara, Diauarum, and Pavuru villages (OR = 8.10, 4.10, 4.88, 1.85, and 1.40, respectively). Conclusions:H. pylori infection is highly prevalent in these communities, as well as intestinal parasitoses. However, there were significant differences in the prevalence of H. pylori among the diverse villages studied. Giardia was closely associated with H. pylori infection. J Pediatr (Rio J). 2011;87(5):393-8: Epidemiology, prevalence,Helicobacter pylori, giardia. ResumoObjetivo: Avaliar a prevalência da infecção por Helicobacter pylori e sua associação com parasitoses intestinais em crianças da comunidade indígena do Parque Indígena do Xingu. Métodos:Foram incluídas 245 crianças indígenas entre 2 e 9 anos, de seis aldeias da região do rio Xingu, afluente do Amazonas. H. pylori foi detectado pelo teste respiratório com ureia-13 C. Foram coletadas amostras de ar expirado, em jejum e 30 minutos após a ingestão de 50 mg de ureia-13 C diluída em 100 mL de água aromatizada com suco de maracujá. Foram coletadas amostras de fezes de 202/245 (82,4%) crianças para exame protoparasitológico. Conclusões: Foi encontrada alta prevalência de H. pylori e de parasitose intestinal em crianças nas comunidades presentemente investigadas. No entanto, houve diferença significativa na prevalência do H. pylori entre as diversas aldeias estudadas. Verificou-se associação entre a presença de giárdia e a infecção por H. pylori.

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“…In Brazil, previous validation of a monoclonal stool antigen test for diagnosis of H. pylori was reported in children 27 ; however, the stool antigen test based on lateral flow chromatography has not yet been tested in adults. The major advantage of these tests is the cost, when compared to 13 C-UBT and the possibility of being performed in any laboratory 10,14,15,17 .…”

Section: Introductionmentioning

confidence: 99%

“…The stool antigen tests based on immunoassay (ELISA) have been tested in several laboratories of the world, including in Brazil 4,[26][27][28] , and the results have been satisfactory. In Brazil, previous validation of a monoclonal stool antigen test for diagnosis of H. pylori was reported in children 27 ; however, the stool antigen test based on lateral flow chromatography has not yet been tested in adults.…”

Section: Introductionmentioning

confidence: 99%

Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection

Silva

Villares

Monteiro

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe aim of this study was to validate the rapid lateral flow Helicobacter pylori stool antigen test (One step H. pylori antigen test, ACON laboratories, San Diego, USA; Prime diagnostics, São Paulo), using 13 C-Urea Breath Test as the gold standard for H. pylori infection diagnosis. A total of 98 consecutive patients, asymptomatic or dyspeptic, entered the study. Sixty-nine were women, with a mean age of 45.76 ± 14.59 years (14 to 79 years). In the H. pylori-positive group, the rapid stool antigen test detected H. pylori antigen in 44 of the 50 positive patients (sensitivity 88%; 95% CI: 75.7-95.5%), and six false-negative; and in the H. pylori-negative group 42 presented negative results (specificity 87.5%; 95% CI: 74.7-95.3%), and six false-positive, showing a substantial agreement (Kappa Index = 0.75; p < 0.0001; 95% CI: 0.6-0.9). Forty four of fifty patients that had positive stool antigen were H. pylori-positive, the PPV of the stool antigen test was 88% (95% CI: 75.7-95.5%), and 42 patients with negative stool antigen test were H. pylori-negative, the NPV of the stool antigen test was 87.5% (95% CI: 74.7-95.3%). We conclude that the lateral flow stool antigen test can be used as an alternative to breath test for H. pylori infection diagnosis especially in developing countries.

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Validation of a Monoclonal Stool Antigen Test for Diagnosing Helicobacter pylori Infection in Young Children

Abstract: The monoclonal stool antigen test is accurate for diagnosing H pylori in children younger than 7 years old, but it must be locally validated in order to find the best cutoff for each population.

Cited by 39 publications

References 37 publications

Is the Immunocromatographic Fecal Antigen Test Effective for Primary Diagnosis of Helicobacter Pylori Infection in Dyspeptic Patients?

Is the Immunocromatographic Fecal Antigen Test Effective for Primary Diagnosis of Helicobacter Pylori Infection in Dyspeptic Patients?

Prevalence of Helicobacter pylori infection and intestinal parasitosis in children of the Xingu Indian Reservation

Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection

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