Validation of a rapid and sensitive HPLC/MS method for measuring sucrose, fructose and glucose in plant tissues

Simultaneous Identification and Dynamic Analysis of Saccharides during Steam Processing of Rhizomes of Polygonatum cyrtonema by HPLC–QTOF–MS/MS

“…All these results demonstrated that the developed quantitative HPLC–QTOF–MS method was linear, precise, stable, sensitive, and accurate enough for the determination of small-molecular saccharides in rhizomes of P. cyrtonema . There are studies using HPLC system coupled with MS to investigate saccharides in plant tissues or products, but previous reports mainly investigated glucose, fructose and sucrose [ 10 , 22 ]. LC–APCI–MS was developed to distinguish saccharides with different types of columns, but it is still hard to separate glucose and galactose [ 13 ].…”

“…Only in a few foods, for instance honey, fructose, and glucose together account for 85–95%, and fructose usually predominates, which is responsible for the sweetness [ 32 ]. The change of fructose could be the main reason why the taste of the rhizomes of P. cyrtonema became sweeter after the steaming processing, since fructose is a major determinant of sweetness in food [ 10 ]. The high content of fructose is also in accordance with our speculation that oligosaccharides were mainly composed of fructose.…”

“…LC is widely used thanks to its various detectors, while NMR cannot be used to detect trace amounts of saccharides, and GC requires tedious derivatization [ 9 ]. However, because the absence of chromophore and fluorophore groups, avoiding direct detection by ultraviolet and fluorescence or diode array detectors, pre-column derivatization is often employed in LC, which is time consuming and could introduce extra variations into the final data [ 10 ]. LC equipped with an evaporative light scattering detector (ELSD) could be used to detect the monosaccharides; for instance, glucose, fructose, and sucrose were examined in the raw materials of agave leaf [ 11 ].…”

“…LC equipped with an evaporative light scattering detector (ELSD) could be used to detect the monosaccharides; for instance, glucose, fructose, and sucrose were examined in the raw materials of agave leaf [ 11 ]. Structural information can even be provided by well-developed analytical techniques; for example, mass spectrometry (MS) coupled with LC is currently a more powerful analytical tool for saccharide analysis [ 10 ]. Sucrose, fructose, and glucose in plant tissues can be unambiguously determined by LC–MS [ 10 ].…”

“…Structural information can even be provided by well-developed analytical techniques; for example, mass spectrometry (MS) coupled with LC is currently a more powerful analytical tool for saccharide analysis [ 10 ]. Sucrose, fructose, and glucose in plant tissues can be unambiguously determined by LC–MS [ 10 ]. Three major tri–saccharides in wheat flour were also determined by LC–MS [ 12 ].…”