Validation of a simple sample preparation method for multielement analysis of bovine serum

Luna, Diego; Miranda, Marta; Minervino, Antônio Humberto Hamad; Piñeiro, Verónica; Herrero-Latorre, Carlos; López‐Alonso, Marta

doi:10.1371/journal.pone.0211859

Cited by 18 publications

(11 citation statements)

References 16 publications

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“…Compared to other studies e.g. Luna D et al [32] we showed better results in accuracy (%) and LOD for most of the elements. In our study we only used 100 μl of serum or whole blood, which make this method also suitable for detecting trace element deficiency or excess in newborns and infants, using capillary blood sampling techniques (collection volume typically > 500 μl).…”

Section: Plos Onecontrasting

confidence: 62%

ICP-MS trace element analysis in serum and whole blood

Laur

Kinscherf

Pomytkin³

et al. 2020

PLoS ONE

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Trace elements and minerals are compounds that are essential for the support of a variety of biological functions and play an important role in the formation of and the defense against oxidative stress. Here we describe a technique, allowing sequential detection of the trace elements (K, Zn, Se, Cu, Mn, Fe, Mg) in serum and whole blood by an ICP-MS method using single work-up, which is a simple, quick and robust method for the sequential measurement and quantification of the trace elements Sodium (Na), Potassium (K), Calcium (Ca), Zinc (Zn), Selenium (Se), Copper (Cu), Iron (Fe), Manganese (Mn) and Magnesium (Mg) in whole blood as well as Copper (Cu), Selenium (Se), Zinc (Zn), Iron (Fe), Magnesium (Mg), Manganese (Mn), Chromium (Cr), Nickel (Ni), Gold (Au) and Lithium (Li) in human serum. For analysis, only 100 μl of serum or whole blood is sufficient, which make this method suitable for detecting trace element deficiency or excess in newborns and infants. All samples were processed and analyzed by ICP-MS (Agilent Technologies). The accuracy, precision, linearity and the limit of quantification (LOQ), Limit of Blank (LOB) and the limit of detection (LOD) of the method were assessed. Recovery rates were between 80-130% for most of the analyzed elements; repeatabilities (C v %) calculated were below 15% for most of the measured elements. The validity of the proposed methodology was assessed by analyzing a certified human serum and whole blood material with known concentrations for all elements; the method described is ready for routine use in biomonitoring studies.

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Section: Plos Onecontrasting

confidence: 62%

ICP-MS trace element analysis in serum and whole blood

Laur

Kinscherf

Pomytkin³

et al. 2020

PLoS ONE

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“…On the contrary, for the minor or only occasionally beneficial trace elements (Cr, Li and Ni) and the toxic elements, lower or not significant associations between plasma and serum concentrations were found. All these elements are at very low concentrations in the blood (close to the detection limits) making their analytical quantification highly influenced by the inter-analysis variation [6,12]. It is also possible that some of these elements could have an affinity to the erythrocytes (as previously stated for Hg and Pb) so that some degree of haemolysis could exert a high influence on the correlation analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Aliquots (1 mL) of plasma and serum samples were digested using an acid digestion procedure, as previously described [6]. Briefly, the plasma or serum samples were digested by mixing with 1 mL of concentrated HNO 3 and 0.5 mL H 2 O 2 in propylene tubes and leaving at 60 °C for 2 h. The digest thus obtained was diluted by adding 2.5 mL of ultrapure water and centrifuging at 2000 rpm for 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…Trace element analysis was previously complicated as it was very time consuming and inaccurate for some elements present in very low concentrations in blood (e.g., Mo and Co). However, modern and multielement inductively coupled plasma mass spectrometry (ICP-MS) techniques, together with the development of sample preparation protocols that prevent matrix-related interference [6], enable the accurate, rapid and relatively inexpensive routine determination of most trace elements by analysis of a single blood sample in multidisciplinary laboratories.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Essential and Toxic Elements in Cattle Blood: Serum vs Plasma

Luna

López‐Alonso

Cedeño

et al. 2019

Animals

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This study was designed to evaluate the influence of type of blood sample (serum or plasma) on essential and toxic element analysis in cattle. Paired plasma and serum samples (n = 20) were acid digested, and the concentrations of As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, Li, Mg, Mn. Mo, Ni, P, Pb, Sb, Se, Sr and Zn were determined by inductively coupled plasma mass spectrometry (ICP-MS). The study findings indicate that plasma and serum samples appear suitable and interchangeable for the determination of most of the essential and toxic elements in blood in cattle. The only exceptions are Cu and Se, the concentrations of which were significantly lower (40.9 and 29.9% respectively) in serum than in plasma. Some of the Cu in blood samples from bovine ruminants is known to be sequestered during clotting. However, further research on Se in ruminants and other animal species is warranted. Finally, the significantly higher Mn (9.9%) concentrations in serum than in plasma may have been caused by haemolysis of some samples. Special attention should be paid to preventing haemolysis of samples during collection and processing, in order to prevent overestimation of elements present at high concentrations inside erythrocytes (i.e., Fe, Mn and Zn).

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“…The digest thus obtained was diluted by adding 2.5 mL of ultrapure water. The sample solutions were then centrifuged at 2000 rpm for 5 min and the concentrations of trace elements (Co, Cr, Cu, Fe, Mn, Mo, Ni, Se and Zn) and toxic elements (As, Cd, Hg and Pb) were subsequently determined in the supernatant, by ICP-MS (Agilent 7700× ICP-MS system; Agilent Technologies, Tokyo, Japan), according to the analytical procedure described in detail in a previous paper [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs

Cedeño

Miranda

Galdo

et al. 2020

Animals

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This study was designed (i) to establish reference ranges for the essential trace element and background levels of toxic element exposure in the healthy/normal dog population, and (ii) to evaluate whether trace element concentrations vary in dogs suffering from different pathologies. Blood serum samples were collected from 187 healthy and diseased dogs at the Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Santiago de Compostela (northwest Spain). The samples were acid digested, and the concentrations of trace elements (Co, Cr, Cu, Fe, Mn, Mo, Ni, Se and Zn) and toxic elements (As, Cd, Hg and Pb) were determined by inductively coupled plasma-mass spectrometry (ICP-MS). This enabled us to establish reference ranges for the essential trace elements and the level of toxic element exposure in dogs, and to identify several clinical situations associated with variations in trace elements in serum. Relative to concentrations in healthy control dogs, statistically significant differences were observed in the concentrations of Cu (significantly higher in hepatic, inflammatory/infectious and oncological categories), Mo (significantly higher in renal category), Se (significantly lower in gastrointestinal category) and Zn (significantly lower in gastrointestinal, inflammatory/infectious and renal categories). Trace element concentrations can be a cause or consequence of disease, and the study findings indicate that trace element determination in serum provides useful information on the pathogenesis of certain diseases. Further research on the serum concentrations of trace elements, particularly in relation to other biochemical parameters and diagnostic tools, may provide valuable information for the diagnosis of diseases in dogs and the disease prognosis.

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Validation of a simple sample preparation method for multielement analysis of bovine serum

Cited by 18 publications

References 16 publications

ICP-MS trace element analysis in serum and whole blood

ICP-MS trace element analysis in serum and whole blood

Determination of Essential and Toxic Elements in Cattle Blood: Serum vs Plasma

Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs

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