Validation of an analytical method to quantify serum electrolytes by atomic absorption spectroscopy

García-Alegría, Alejandro Monserrat; Gómez-Álvarez, Agustín; García-Rico, Leticia; Serna-Félix, Mercedes

doi:10.15174/au.2015.747

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Due to their robustness and low cost, electrochemical methods or colorimetry/complexometric titration incorporated in biochemical analyzers have been widely used to determine the concentrations of serum electrolytes [ 1 , 2 , 3 ]. Atomic absorption spectroscopy (AAS) is also an accurate analysis method, but it has not been massively used in clinical practice due to its complex analysis process [ 4 ]. Inductively coupled plasma optical emission spectrometry (ICP-OES) and mass spectrometry (ICP-MS) provide sensitive methods for elemental analysis, but they need cumbersome sample preparation and cost too much [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Electrolyte Analysis in Blood Serum by Laser-Induced Breakdown Spectroscopy Using a Portable Laser

Feng

et al. 2022

Molecules

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The fast and reliable analysis of electrolytes such as K, Na, Ca in human blood serum has become an indispensable tool for diagnosing and preventing diseases. Laser-induced breakdown spectroscopy (LIBS) has been demonstrated as a powerful analytical technique on elements. To apply LIBS to the quantitative analysis of electrolyte elements in real time, a self-developed portable laser was used to measure blood serum samples supported by glass slides and filter paper in this work. The partial least squares regression (PLSR) method was employed for predicting the concentrations of K, Na, Ca from serum LIBS spectra. Great prediction accuracies with excellent linearity were obtained for the serum samples, both on glass slides and filter paper. For blood serum on glass slides, the prediction accuracies for K, Na, Ca were 1.45%, 0.61% and 3.80%. Moreover, for blood serum on filter paper, the corresponding prediction accuracies were 7.47%, 1.56% and 0.52%. The results show that LIBS using a portable laser with the assistance of PLSR can be used for accurate quantitative analysis of elements in blood serum in real time. This work reveals that the handheld LIBS instruments will be an excellent tool for real-time clinical practice.

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Section: Introductionmentioning

confidence: 99%

Electrolyte Analysis in Blood Serum by Laser-Induced Breakdown Spectroscopy Using a Portable Laser

Feng

et al. 2022

Molecules

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“…Inductively coupled plasma mass spectrometry (ICP-MS), inductively coupled plasma optical emission spectroscopy (ICP-OES), and atomic absorption spectrometry (AAS) are the main detection methods for blood electrolytes. [7][8][9][10][11] Although these methods have high detection sensitivity and accuracy, they require a complex sample preparation process, such as acid digestion and dilution, thus increasing the detection time and generating chemical waste.…”

Section: Introductionmentioning

confidence: 99%

Fast determination of electrolyte elements in human blood plasma using surface-enhanced laser-induced breakdown spectroscopy combined with a gel film method

Wang

Yang

et al. 2023

J. Anal. At. Spectrom.

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“…The test results show that the percentage of recovery (%R) was 98% ± 3%. According to Garcia-Alegria et al (2015) , a method has a high accuracy if the recovery ranges from 95% to 105%.…”

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confidence: 99%

VALIDATION OF LEAD (Pb) ANALYSIS METHOD IN FISH MEAL MATRIX BY ELECTROTHERMAL ATOMIC ABSORPTION SPECTROPHOTOMETRY

Suwari

Ledoh

Rozari

2021

Int. J. Eng. Sci. Tech

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ABSTRACT Validation of analytical methods is the process of proving that the methods used are scientifically good and suitable for certain purposes. The aim of this study was to validate the analytical method of lead in fish meal matrix by electrothermal atomic absorption spectroscopy (ETAAS) using a phosphate modifier. The research begins with optimization of the analytical conditions that include pyrolysis (ashing) and atomizing temperature, and continues with the validation process by evaluating the analytical performance parameters using a certified reference material (CRM). Method validation was carried out by determining the level of accuracy and precision as well as lead content in a CRM Cod Muscle-422 from BCR. Each experiment was conducted at a wavelength of 283.3 nm, a lamp current of 9.0 amperes, a slit width of 1.3 nm and an argon carrier gas. The optimal pyrolysis and atomizing temperature of lead obtained by adding phosphate modifier was 650 oC and 2000 oC, respectively. The results obtained in this study indicate that the ETAAS lead analysis method using a phosphate modifier is feasible as a test method with an RSD of 3.79%, precision of 0.0043, relative error of 4.0%, accuracy of 104% and recovery of 98% ± 3%. The results of the validation method obtained that the 95% confidence interval for Pb content was 0.088 ± 0.023 µg/g, still within the range of Pb levels in the certificate, namely 0.085 ± 0.015 µg/g of Pb. Keywords: Validation, CRM, Cod Muscle, ETAAS, Lead

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Validation of an analytical method to quantify serum electrolytes by atomic absorption spectroscopy

Cited by 4 publications

References 15 publications

Electrolyte Analysis in Blood Serum by Laser-Induced Breakdown Spectroscopy Using a Portable Laser

Electrolyte Analysis in Blood Serum by Laser-Induced Breakdown Spectroscopy Using a Portable Laser

Fast determination of electrolyte elements in human blood plasma using surface-enhanced laser-induced breakdown spectroscopy combined with a gel film method

VALIDATION OF LEAD (Pb) ANALYSIS METHOD IN FISH MEAL MATRIX BY ELECTROTHERMAL ATOMIC ABSORPTION SPECTROPHOTOMETRY

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