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ABSTRAKTelah dilakukan uji aktivitas antibakteri Eschericia coli (E. coli) minyak batang genoak (Acorus calamus). Penelitian ini dilakukan melalui destilasi uap batang genoak yang diikuti analisis komposisi kimianya menggunakan GC-MS, dan uji antibakteri terhadap bakteri E. coli menggunakan metode hitung cawan. Hasil penelitian diperoleh minyak genoak dengan rendemen 0,17% dan asaron sebagai komponen utama minyak genoak sebesar 89,81%. Minyak genoak dapat menghambat pertumbuhan bakteri E. coli sebesar 95,76% pada kosentrasi 10% (v/v).Kata kunci : Destilasi uap, Batang Genoak (Acorus calamus), E. coli, Asaron. THE ANTI Eschericia coli STUDY OF GENOAK(Acorus calamus) OIL FROM TIMOR ABSTRACTThe study of anti Eschericia coli (E. coli) of genoak oil (Acorus calamus) has been done. The research aimed to emerge the potential characteristic of genoak oil which distilled from its stem part. The anti bacterial study was done using plate count method, whilist the chemical compound contained in the oil was investigated using GC-MS. The research result show that asaron in the major compound in stem part of genoak i.e. 89.81%. The genoak oil inhibited 95.76% of the E. coli growth at 10% (v/v) of its concentration.

Constructed wetlands are a promising solution technology to effectively treat domestic wastewater in developing countries at low cost. This paper reports the findings of the effectiveness of sand planted with Thypa latifolia with variation of the length of outflow measured from the bottom of the media in removing BOD 5 , COD, and suspended solids. The experimental design consisted of 12 vertical flow (VF) mesocosms. There were 3 treatments and one control based on the with variation of the length of outflow measured from the bottom of the media (7, 14, and 21 cm). During the five months, the mesocosms were loaded with syntethic wastewater. The influent had a 2-day hydraulic retention time. Samples were monitored for BOD 5 , COD and TSS. The results showed the concetration of BOD 5 , COD and TSS reduced significantly after the wastewater were treated with constructed wetland systems. The trend showed that the constructed wetland systems planted with Thypa latifolia had a better performance in comparison with the control. There were no significant differences of BOD 5 COD, and suspended solidoutflow among 7, 14 and 21 of constructed wetland systems. This indicated that the length of outflow measured from the bottom of the media did not influence the performance of constructed wetland systems in removing BOD 5 , COD and TSS.