Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection

Pena, Angelina; Pelantova, N.; Lino, Celeste M.; Silveira, Maria Irene Noronha da; Solich, Petr

doi:10.1021/jf050065r

Cited by 76 publications

(63 citation statements)

References 10 publications

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“…However, Pena et al found that COOH treatment was not suitable or stable for several honeys due to the large variation in the pH in the extracts. They recommended propylsulfonate (PRS) cartridge treatment, which was not a#ected by pH because of its strong cationic exchange characteristics 13) . We confirmed the e#ectiveness of PRS on some dark-colored honeys, but it was ine#ective on buckwheat honey.…”

Section: Clean-up Proceduresmentioning

confidence: 99%

“…However, the above methodology could not be used for certain honeys because of their strong matrix e#ects. To remove matrix e#ects of dark-colored honeys, a more rigorous clean-up procedure is required 4), 8), 13) . Although metal chelate a$nity chromatography (MCAC) has been reported as an e#ective purification technique of TCs in livestock products 18)ῌ22) , MCAC cleanup could not solve the problem of matrix e#ects from bee-related products for the following reason.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Trace Residues of Tetracyclines in Dark-Colored Honeys by High-Performance Liquid Chromatography Using Polymeric Cartridge and Metal Chelate Affinity Chromatography

Fujita¹,

Ito²,

Ishihara³

et al. 2008

J. Food Hyg. Soc. Jpn

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An e$cient clean-up procedure was developed for the trace residue determination of tetracyclines (TCs) in dark-colored honeys. TCs were extracted from samples with McIlvaine bu#er (pH 4.0) containing 0.01 mol/L Na 2 EDTA. The extracts were treated with both a polymeric cartridge (GL-Pak PLS-2) and a metal chelate a$nity column (MCAC) preloaded with copper(II). TCs were eluted and analyzed by high-performance liquid chromatography (HPLC) using fluorescence detection. The method was evaluated for the determination of oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) in buckwheat honey, because its color is the darkest. The mean recoveries of OTC, TC and CTC from spiked samples, at three fortification levels, were ῍70῎, and the relative standard deviations (RSDs) were ῌ10῎. Limits of quantitation (LOQs) of OTC, TC, and CTC were estimated to be 0.015 mg/kg, 0.019 mg/kg, and 0.024 mg/kg, respectively.

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Section: Clean-up Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Trace Residues of Tetracyclines in Dark-Colored Honeys by High-Performance Liquid Chromatography Using Polymeric Cartridge and Metal Chelate Affinity Chromatography

Fujita¹,

Ito²,

Ishihara³

et al. 2008

J. Food Hyg. Soc. Jpn

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“…(UV), 9,10 fluorescence, 14,15 chemiluminescence 16 and mass antibiotics, which are commonly used in veterinary medicine to spectrometry. [17][18][19] One major obstacle in the detection of TCs prevent diseases and also to promote growth, 1 takes a central by HPLC is the presence of impurities in honey.…”

Section: Introductionmentioning

confidence: 99%

Practical Method for the Detection of Tetracyclines in Honey by HPLC and Derivative UV-Vis Spectra

2009

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A technique to detect residual tetracyclines (TCs) in honey by HPLC and derivative UV-Vis spectra is examined in this study. Chromatograms of honey products often experience overlapped peaks and sloping background due to similar retention properties of components or to the presence of impurities. The derivatives provide resolution of overlapped peaks in an UV-Vis spectrum and eventually bring chromatograms that show pure elution behavior of TCs. Calibration models for oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) are built out of the derivative spectra of standard solutions of honey and their prediction abilities are examined. The results show that the calibration models for OTC, TC and CTC can yield sufficient levels for the determination. The technique reported here may be effectively used for quantitative determination of TCs.

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“…These include: microbiological assay (Nagel et al 2011), differential scanning calorimetry (DSC) (Yildiz and Unluturk 2009), capillary electrophoresis (CE) (Ibarra et al 2011), enzyme-linked immunosorbent assays (ELISA), radioimmunoassays, biosensors and chromatographic techniques, such as high-performance liquid chromatography (HPLC), coupled with different detection systems (Toldrá and Reig 2006), liquid chromatography operating under reverse phase mode (Aderson et al 2005) and coupled with several detection schemes such as spectrophotometry (Viñas et al 2004;Andersen et al 2005;Fritz and Zuo 2007), electrochemical (Casella and Picerno 2009), fluorescence (Pena et al 2005;Pena et al 2007;Schneider et al 2007), or mass spectrometry (Andersen et al 2005;Pena et al 2007), among others.…”

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confidence: 99%

Mid-infrared spectroscopy and multivariate analysis for determination of tetracycline residues in cow's milk

et al. 2018

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Mid-infrared spectroscopy and chemometric analysis were tested to determine tetracycline's residues in cow's milk. Cow's milk samples (n = 30) were spiked with tetracycline, chlortetracycline, and oxytetracycline in the range of 10-400 µg/l. Chemometric models to quantify each of the tetracycline's residues were developed by applying Partial Components Regression and Partial Least Squares algorithms. The Soft Independent Modeling of Class Analogy model was used to differentiate between pure milk and milk sample with tetracycline residues. The best models for predicting the levels of these antibiotics were obtained using Partial Least Square 1 algorithm (coefficient of determination between 0.997-0.999 and the standard error of calibration from 1.81 to 2.95). The Soft Independent Modeling of Class Analogy model showed well-separated groups allowing classification of milk samples and milk sample with antibiotics. The obtained results demonstrate the great analytical potential of chemometrics coupled with mid-infrared spectroscopy for the prediction of antibiotic in cow's milk at a concentration of microgram per litre (µg/l). This technique can be used to verify the safety of the milk rapidly and reliably. Antibiotics, human health, multivariate analysis, chemometrics, vibrational spectral dataThe tetracyclines (TCs) have a wide antibacterial range and bacteriostatic action, and a good activity against infections caused by a number of bacteria. The TCs are permitted in a diversity of food-producing animals (sheep, poultry, pigs, cattle and fish) (Debuf 1998). The usage of TCs in dairy husbandry and the failure to follow good veterinary practices can lead to unsafe residue concentrations in various tissues and milk, with potential adverse effects on human health (for example allergic reactions in some hypersensitive individuals). Also the TCs residues may affect aquatic ecosystems (Feng-Jiao et al. 2014).To guarantee high quality of human food, several regulatory authorities have defined the maximum residue limits (MRLs) allowing only trace amounts of residues of veterinary drugs in food for human intake. The MRLs are based on the acceptable daily intake of each drug; maximum food intake is also taken into account. The MRLs are established at mg·kg -1 or µg·kg -1. The US Food and Drug Administration (FDA) has set tolerances for the residues of tetracycline, chlortetracycline and oxytetracycline in bovine milk at 300 μg·l Avoidance of TC residues is an important focus of the dairy industry. Judicious use of antibiotics, followed by an appropriate milk withholding time and screening for residues are essential aspects of milk quality management for assuring human food safety (Boeckman and Carlson 2006). Consequently, it is important to develop analytical procedures capable of determining the levels of TCs and to evaluate their presence in milk in order to protect human health (Casella and Picerno 2009).

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Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection

Cited by 76 publications

References 10 publications

Analysis of Trace Residues of Tetracyclines in Dark-Colored Honeys by High-Performance Liquid Chromatography Using Polymeric Cartridge and Metal Chelate Affinity Chromatography

Analysis of Trace Residues of Tetracyclines in Dark-Colored Honeys by High-Performance Liquid Chromatography Using Polymeric Cartridge and Metal Chelate Affinity Chromatography

Practical Method for the Detection of Tetracyclines in Honey by HPLC and Derivative UV-Vis Spectra

Mid-infrared spectroscopy and multivariate analysis for determination of tetracycline residues in cow's milk

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