Validation of an LC–MS/MS assay of terpene trilactones in Ginkgo biloba extracts and pharmaceutical formulations through standard addition method

Sora, Daniela Iuliana; Stefanescu, Venera; David, Víctor; Medvedovici, Andrei

doi:10.1016/j.jpba.2009.05.040

Cited by 25 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, some procedures for determining these compounds are already reported in the literature. For example, Sora et al . determined terpene lactones in Ginkgo biloba extracts and pharmaceutical formulations using liquid chromatography coupled to mass spectrometry (MS).…”

Section: Introductionmentioning

confidence: 99%

A liquid chromatography-atmospheric pressure photoionization tandem mass spectrometric (LC-APPI-MS/MS) method for the determination of triterpenoids in medicinal plant extracts

et al. 2016

View full text Add to dashboard Cite

An analytical method using liquid chromatography-atmospheric pressure photoionization tandem mass spectrometry with toluene as a dopant was developed for the determination of triterpenes in medicinal plant extracts. The 12 compounds determined have been shown to exhibit biological activity, such as gastroprotective, hepatoprotective, anti-inflammatory, antiviral and anti-tumor effects. The parameters of the atmospheric pressure photoionization interface were optimized to obtain the highest possible sensitivity for all of the compounds. The limits of detection and quantification ranged from 0.4 to 157.9 µg l and 1.3 to 526.4 µg l , respectively. The method was validated and applied to extracts of five medicinal plants species (Mansoa alliacea (Lam.) A.H.Gentry, Bauhinia variegata var variegata, Bauhinia variegata var alboflava, Cecropia obtuse Trécul and Cecropia palmate Willd) from the Amazonian region. The concentrations of the six triterpenes quantified in the samples ranged from 0.424 mg kg for ursolic acid to 371.96 mg kg for β-amyrin, which were quantified by using the standard addition method (n = 3). Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Section: Introductionmentioning

confidence: 99%

A liquid chromatography-atmospheric pressure photoionization tandem mass spectrometric (LC-APPI-MS/MS) method for the determination of triterpenoids in medicinal plant extracts

et al. 2016

View full text Add to dashboard Cite

show abstract

“…For the determination of Ginkgo biloba constituents in the leaves, extracts, pharmaceutical formulations, and plasma, different GC/ MS and LC/MS (ESI or APCI) methods are published [12,[14][15][16][17][18][19].…”

mentioning

confidence: 99%

Plasma and Brain Levels of Terpene Trilactones in Rats after an Oral Single Dose of StandardizedGinkgo bilobaExtract EGb 761®

Ude

Paulke²,

Nöldner³

et al. 2010

Planta Med

View full text Add to dashboard Cite

Several studies indicate that the terpene trilactones (TTL) of EGb 761® are responsible for most of its pharmacological action in the brain . Therefore, we investigated the ability of the TTL to cross the blood brain barrier in rats after a single oral administration (600 mg/kg) of EGb 761® and compared it with the plasma levels. In addition, we checked the pharmacokinetic characteristics of an application of EGb 761® against a similar amount of pure substances. For this purpose, we developed a sensitive HPLC-(APCI)-MS method for the determination of the Ginkgo biloba TTL (ginkgolide A [GA], B [GB], C [GC] and bilobalide [Bb]) in plasma as well as in brain tissue. The following animal study shows that the oral application of 600 mg/kg EGb 761® results in significant GA, GB, and Bb concentrations in plasma as well as in the CNS of the rodents, while the GC concentration was below the detection limit of the analytical method in both matrices. GA, GB, and Bb brain concentrations showed a rapid increase up to 55 ng/g, 40 ng/g, and 98 ng/g with no difference of the characteristic after extract or pure substance application. Regarding the plasma levels, significant higher C(max) and AUC values were detected after application of the extract EGb 761®. These results allow for the first time a discussion of pharmacological effects with the knowledge of the pharmacokinetic behavior of the TTL in target tissues.

show abstract

“…S1). Sora, Stefanescu, David, and Medvedovici (2009) also revealed the efficacy of the standard addition method-aided LC-MRM-MS/MS assay in generating reproducible ionization conditions with the successful quantification of five terpene trilactones from Ginkgo extracts. However, by considering our current objective, i.e., the establishment of a convenient and reliable quantitative LC-MS assay for bioactive dipeptides, in this study we chose to exclude these three dipeptides as quantitative single LC-TOF-MS targets in the soybean hydrolysate.…”

Section: Esi-ms Detection Of Tnbs-derivatized and Non-derivatized Dipmentioning

confidence: 95%

Quantitative mass spectrometric analysis of dipeptides in protein hydrolysate by a TNBS derivatization-aided standard addition method

et al. 2016

View full text Add to dashboard Cite

Validation of an LC–MS/MS assay of terpene trilactones in Ginkgo biloba extracts and pharmaceutical formulations through standard addition method

Cited by 25 publications

References 19 publications

A liquid chromatography-atmospheric pressure photoionization tandem mass spectrometric (LC-APPI-MS/MS) method for the determination of triterpenoids in medicinal plant extracts

A liquid chromatography-atmospheric pressure photoionization tandem mass spectrometric (LC-APPI-MS/MS) method for the determination of triterpenoids in medicinal plant extracts

Plasma and Brain Levels of Terpene Trilactones in Rats after an Oral Single Dose of StandardizedGinkgo bilobaExtract EGb 761®

Quantitative mass spectrometric analysis of dipeptides in protein hydrolysate by a TNBS derivatization-aided standard addition method

Contact Info

Product

Resources

About