Validation of biomarkers of marine pollution exposure in sand flathead using Aroclor 1254

Brumley, C.M.; Haritos, Victoria S.; Ahokas, Jorma T.; Holdway, Douglas A.

doi:10.1016/0166-445x(94)00074-z

Cited by 33 publications

(13 citation statements)

References 33 publications

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“…Ž . Brumley et al 1995 used the same concentration and duration period of MS-222 on sand flathead as in the present study, and reported no effect on EROD levels. The use of anaesthesia was necessary for blood sampling and injections to be carried out while allowing the handling of vigorous and spiny fish.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies in which sand flathead were ip injected with 400 mgrkg of Aroclor 1254 showed maximum EROD induction increases of Ž . only fivefold Brumley et al, 1995 . The present study was able to induce EROD activity by up to 20-fold in the PCB injected group, relative to controls, indicating that sand flathead can be an excellent biomonitoring tool.…”

Section: Discussionmentioning

confidence: 99%

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MFO Induction of three Australian fish species

Smith

Gagnon

2000

Environ. Toxicol.

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The use of mixed function oxygenase MFO enzymes as a biomarker of pollution in Port ( ) Phillip Bay was investigated in three indigenous fish species, sand flathead Plathycephalus bassensis , ( ) ( ) bluethroat wrasse Notalabrus tetricus , and sixspine leatherjacket Meuschenia freycineti . Investigation of the relative MFO induction potential was performed by intraperitoneally injecting each experimental X X ( ) fish species with 100 g / kg of 3,3 ,4,4 ,5-pentachlorobiphenyl PCB 126 using corn oil as a carrier. Hepatic induction was fluorimetrically measured by the activity of 7-ethoxyresorufin O-deethylase ( ) EROD activity 10 days post-injection. Of the three species tested, the sand flathead was selected as the most suitable indicator species for its high MFO induction potential and desirable characteristics related to its population biology. Bluethroat wrasse appeared relatively uninducible by PCB 126, while sixspine leatherjacket demonstrated a medium induction potential but was unsuitable as a biomonitoring tool because of its scarcity and distribution limited to reef beds.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MFO Induction of three Australian fish species

Smith

Gagnon

2000

Environ. Toxicol.

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“…Cytochrome P450 assay P450 content was analyzed by the dithionite reduced difference spectrum of carbon monoxide (CO) bubbled samples, according to the method of Matsubara et al (1976), with modifications from Rutten et al (1987) and described in Brumley et al (1995). Samples were read on a Shimadzu UV-3000 dual wavelengthldouble beam spectrophotometer.…”

Section: Microsome Preparationmentioning

confidence: 99%

A preliminary investigation into the use of biomarkers for the monitoring of an ocean outfall

Mosse

Brumley

Ahokas

et al. 1996

Environ. Toxicol. Water Qual.

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“…Racotta and Hernandez-Herrera (2000) found a significantly higher protein content in hemolymph of white shrimp Penaeus vannamei exposed to 1.07 mmol/L of ammonia-N than in control group. Brumley et al (1995) reported a 1.5-fold increase in liver protein content when injecting sand flathead with 400 mg/kg Aroclor-1254 and De Coen and Janssen (1997) found significant increases in protein reserves in Daphnia magna when exposing them to different concentrations of cadmium, tributyltin, linear alkyl sulfonic acid, lindane and 2,4-dichlorophenoxy acetic acid (2,4-D). It appears that low to intermediate levels of pollution trigger increased protein synthesis (e.g.…”

Section: Changes In Energy Budgetsmentioning

confidence: 99%

Exposure to agricultural pesticides impairs growth, feed utilization and energy budget in African Catfish Clarias gariepinus (Burchell, 1822) fingerlings

et al. 2014

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The African catfish Clarias gariepinus is a widespread species in the Beninese cotton basin. In this study, the impacts of exposure to agricultural pesticides endosulfan [Thionex 350 EC (emulsifiable concentrate)] and Tihan 175 O-TEQ (oil toxicity equivalent) on growth, feed utilization and energy budget of C. gariepinus were investigated. Fingerlings (1.58 ± 0.02 g) were exposed to borehole water (control), 0.23 ppb (environmental concentration), 440 ppb (Lethal Concentration 50 %/20, LC50/20) and 880 ppb (LC50/10) of Tihan; and to 11 ppb (LC50/20), 22 ppb (LC50/10) and 29.40 ppb (environmental concentration) of Thionex for 28 days. Fish biomass was assessed weekly and fish samples were taken from different aquaria to determine the specific growth rate (effect on growth), feed efficiency rate and protein efficiency ratio (impact on feed utilization), and the biochemical composition of fish (impact on the energy budget). The results showed that endosulfan environmental concentration induced 100 % of mortality in catfish fingerlings while mortality rate was comparable between control fish and Tihan-treated fish over the 28-day period (p [ 0.05). In contrast to survival, the two pesticide types tested induced a marked decrease in growth only during the first 2 weeks of exposure (p \ 0.05). The negative impact of endosulfan on growth was associated to a lower feed utilization and protein efficiency compared with control fish or those exposed to Tihan (p \ 0.05). The energy reserves were more rapidly exhausted in fish exposed to endosulfan to meet energy demand generated by this chemical stressor.

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Validation of biomarkers of marine pollution exposure in sand flathead using Aroclor 1254

Cited by 33 publications

References 33 publications

MFO Induction of three Australian fish species

MFO Induction of three Australian fish species

A preliminary investigation into the use of biomarkers for the monitoring of an ocean outfall

Exposure to agricultural pesticides impairs growth, feed utilization and energy budget in African Catfish Clarias gariepinus (Burchell, 1822) fingerlings

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