Validation of FHH-GhsrM1Mcwi GHSR KO rat as a model to study ghrelin biology

Charbonneau, Valerie

doi:10.22215/etd/2012-06893

Cited by 2 publications

(1 citation statement)

References 59 publications

(120 reference statements)

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“…Previous work has shown that FHH-GHSR KO rats show attenuated post fast feeding after a period of food deprivation compared to their WT counterparts because of their insensitivity to ghrelin due to the presence of a mutated GHSR receptor. The deletion of GHSR does not affect overall leptin levels in circulation although GHSR KO have less plasma triglyceride compared to FHH WT rats (Charbonneau, 2012). In addition, growth hormone (GH) secretion is similar in both types of FHH rats suggesting that there must be other factors present to mediate growth hormone secretion.…”

Section: Rationale For Present Thesismentioning

confidence: 99%

The Effect of Fasting on LH Secretion in Female Rats with Mutations to the Ghrelin Receptor Gene

Sabrin¹

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We investigated how ghrelin can mediate the inhibitory effects of fasting on the reproductive axis using female rats with a point mutation to the gene encoding GHSR.For this purpose, we fasted FHH GHSR KO rats and their WT counterparts starting on the evening of metestrus and ending 42h later on the expected proestrus afternoon. Rats were then euthanized; blood was collected and analyzed to compare plasma LH and estrogen concentrations between these animals and controls. Fasting significantly decreased LH concentrations in all rats regardless of their genotype. Interestingly, GHSR KO rats showed overall higher LH concentrations than WT rats regardless of their condition. Estrogen levels were not influenced by the genetic mutation and fasting. These findings suggest that ghrelin signalling is not required for a fastinginduced decrease in LH concentrations. However, ghrelin may modulate reproductive function as a negative feedback signal that reduces LH release regardless of energy state.iii

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Section: Rationale For Present Thesismentioning

confidence: 99%