Validation of HPLC stability-indicating method for Vitamin C in semisolid pharmaceutical/cosmetic preparations with glutathione and sodium metabisulfite, as antioxidants

Maia, Adriana Moura; Baby, André Rolim; Yasaka, Wilson J.; Suenaga, Eunice Mayumi; Kaneko, Telma Mary; Velasco, Maria Valéria Robles

doi:10.1016/j.talanta.2006.05.006

Cited by 50 publications

(34 citation statements)

References 15 publications

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“…Diluted solutions were prepared in replicates of three and the standard curve was constructed plotting peak areas versus concentrations as described previously. The least-squares fit method was employed to statistically evaluate the results for linearity by a regression line and the corresponding slope, yintercept and coefficient of linear correlation (r 2 ) (Maia et al, 2007).…”

Section: Linearity and Specificitymentioning

confidence: 99%

Photostability evaluation of five UV-filters, trans-resveratrol and beta-carotene in sunscreens

Freitas

Lopes

Gaspar

2015

European Journal of Pharmaceutical Sciences

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Section: Linearity and Specificitymentioning

confidence: 99%

Photostability evaluation of five UV-filters, trans-resveratrol and beta-carotene in sunscreens

Freitas

Lopes

Gaspar

2015

European Journal of Pharmaceutical Sciences

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“…[21] High-performance liquid chromatography stability-indicating methods are particularly attractive for the determination of active substances (e.g., ascorbic acid) and for the quantification of potentially occurring degradates in pharmaceutical/cosmetic preparations, developed as oil-in-water emulsion and aqueous gel. [22] Such a method presents convenience, rapidity and the ability to separate substances quantitatively without pre-derivatization.…”

Section: The State-of-the-art In Chromatographic Investigations Towarmentioning

confidence: 99%

Sample Preparation for Trace Analysis by Chromatographic Methods

Olariu

Vione

Grinberg

et al. 2010

Journal of Liquid Chromatography & Related Technologies

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The determination of trace analytes in complex natural matrices often requires extensive sample extraction and preparation prior to chromatographic analysis. Correct sample preparation can reduce analysis time, sources of error, enhance sensitivity and enable unequivocal identification, confirmation and quantification. This overview considers general aspects on sample preparation techniques for trace analysis in various matrices. The discussed extraction/enrichment techniques cover classical methods, such as Soxhlet and liquid-liquid extractions along with more recently developed techniques like pressurized liquid extraction, liquid phase microextraction (LPME), accelerated microwave extraction, and ultrasoundassisted extraction. This overview also deals with more selective methodologies, such as solid phase extraction (SPE), solid phase microextraction (SPME) and stir bar sorptive extraction (SBSE). The adopted approach considers the equilibriums involved in each technique. The applicability of each technique in environmental, food, biological and pharmaceutical analyses is discussed, particularly for the determination of trace organic compounds by chromatographic methods.

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“…To avoid possible interference by these additives in the determination of 1 in these preparations, it was proposed to carry out a separation by RP-HPLC with UVD at 254 nm using MeOH/MeCN containing metaphosphoric acid (pH 2.8). The method was validated for mixtures of the analyte with nicotinic acid (46a), 45, oxalic acid and excipients of topical formulations such as oil in water emulsion or aqueous gel 181 . A kinetic method for determination of ascorbic acid/ascorbate (1) is based on measuring the initial rate of its oxidation to dehydroascorbic acid (18) in phosphate buffer (pH 5 to 6) catalyzed by ascorbate oxidase.…”

Section: Determination Of Ascorbates By Hplcmentioning

confidence: 99%