Validation of IgY for the diagnosis of Streptococcus agalactiae-caused endocarditis and bacterial meningitis in Nile tilapia (Oreochromis niloticus)

Eto, Silas F.; Fernandes, Dayanne Carla; Moraes, Alessandra Cristina de; Prado, E. J. R.; Baldassi, Amanda Cristina; Manrique, Wilson Gómez; Silva, Ives C.; Medeiros, Andrea; Belo, Marco Antônio de Andrade; Balbuena, Tiago Santana; Samara, Samir Issa; Pizauro, João Martins

doi:10.1016/j.fsi.2018.02.048

Cited by 20 publications

(13 citation statements)

References 33 publications

(32 reference statements)

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“…In order to evaluate the application of the compounds against bacteria, the antibacterial potential was tested in human pathogenic bacteria: S. aureus, E. faecalis, and E. coli, as well as in some bacteria species that cause disease in fish and humans (S. agalactiae and A. PLOS ONE hydrophila). The bacteria species S. agalacitiae and A. hydrophila are pathogenic for the fish Nile tilapia (Oreochromis niloticus), causing a foodborne disease in humans [81,82]. Often found on the skin and nasal fossae of healthy individuals S. aureus is a Gram-positive bacterium, considered an opportunistic microorganism and is one of the major causative agents of community and hospital infections worldwide [83] Several diseases are related to this bacterium, from superficial infections of the skin to more serious and deep infections, such as endocarditis, bacteremia, peritonitis, meningitis, necrotizing pneumonia and septic arthritis [84].…”

Section: Plos Onementioning

confidence: 99%

Antimicrobial activity of RP-1 peptide conjugate with ferrocene group

et al. 2020

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Parasitic diseases are a neglected and serious problem, especially in underdeveloped countries. Among the major parasitic diseases, Leishmaniasis figures as an urgent challenge due to its high incidence and severity. At the same time, the indiscriminate use of antibiotics by the population is increasing together with resistance to medicines. To address this problem, new antibiotic-like molecules that directly kill or inhibit the growth of microorganisms are necessary, where antimicrobial peptides (AMPs) can be of great help. In this work, the ferrocene molecule, one active compound with low levels of in vivo toxicity, was coupled to the N-terminus of the RP1 peptide (derived from the human chemokine CXCL4), aiming to evaluate how this change modifies the structure, biological activity, and toxicity of the peptide. The peptide and the conjugate were synthesized using the solid phase peptide synthesis (SPPS). Circular dichroism assays in PBS showed that the RP1 peptide and its conjugate had a typical spectrum for disordered structures. The Fc-RP1 presented antiamastigote activity against Leishmania amazonensis (IC 50 = 0.25 μmol L -1 ). In comparison with amphotericin B, a second-line drug approved for leishmaniasis treatment, (IC 50 = 0.63 μmol L -1 ), Fc-RP1 was more active and showed a 2.5-fold higher selectivity index. The RP1 peptide presented a MIC of 4.3 μmol L -1 against S. agalactiae, whilst Fc-RP1 was four times more active (MIC = 0.96 μmol L -1 ), indicating that ferrocene improved the antimicrobial activity against Gram-positive bacteria. The Fc-RP1 peptide also decreased the minimum inhibitory concentration (MIC) in the assays against E. faecalis (MIC = 7.9 μmol L -1 ), E. coli (MIC = 3.9 μmol L -1 ) and S. aureus (MIC = 3.9 μmol L -1 ). The cytotoxicity of the compounds was tested against HaCaT cells, and no significant activity at the highest concentration tested (500 μg. mL -1 ) was observed, showing the high potential of this new compound as a possible new drug. The coupling of ferrocene also increased the vesicle permeabilization of the peptide, showing a direct relation between high peptide concentration and high carboxyfluorescein release, which indicates the action mechanism by pore formation on the vesicles. Several studies have shown that ferrocene destabilizes cell membranes through lipid peroxidation, leading to cell lysis. It is noteworthy that the Fc-RP1 peptide synthesized here PLOS ONE

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Section: Plos Onementioning

confidence: 99%

Antimicrobial activity of RP-1 peptide conjugate with ferrocene group

et al. 2020

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“…The fish adults were exposed to BENZ, NP and NPMA for 5minutes to concentrations of 25 and 50 mg.L -1 and after 24 and 96 hours, zebrafish were necropsied to collect liver, kidney, spleen, and gills for histopathological analysis. Sections with 6 μm thickness were mounted on slides and stained with hematoxylin-eosin (HE) for observation of general cell structures [34].…”

Section: Histopathological Evaluationmentioning

confidence: 99%

Potential of mucoadhesive nanocapsules in drug release and toxicology in zebrafish

et al. 2020

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Mucoadhesive polymeric nanocapsules have attracted interest of researchers from different fields from natural sciences because of their ability to interact with the mucosa and increase drug permeation. Anesthesia by immersion causes absorption through the skin and gills of fish, so it is important to evaluate the exposure of these organs to drug nanosystems. Benzocaine (BENZ) is one of the most popular anesthetic agents used in fish anesthesia, but it has drawbacks because of its low bioavailability, resulting in weak absorption after immersion. Here we describe method developed for preparing and characterizing chitosan-coated PLGA mucoadhesive nanoparticles containing BENZ (NPMAs) for zebrafish immersion anesthesia. We determined the lowest effective concentration, characterized the interaction of the mucoadhesive system with fish, measured the anesthetic efficacy, and evaluated possible toxic effects in embryos and adults exposed to the nanoformulations. This study opens perspectives for using nanoformulations prepared with BENZ in aquaculture, allowing reduction of dosage as well as promoting more effective anesthesia and improved interaction with the mucoadhesive system of fish.

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“…Both types of bacteria attacked tilapia in various ages and stages (Hardi, Harris, & Lusiastuti, 2011;. The mechanism of infection caused by S. agalactiae bacteria starts from the systemic circulation to the central nervous system, related to the released virulence factors such as polysaccharide capsules, phili, serine-rich (SR) proteins, β-hemolysin, cytolysin, fibronectin and teichoid acid (Eto et al, 2018;Hardi et al, 2011;Lemire, Houde, & Segura, 2012). Streptococcus agalactiae also has virulent genes namely hylB, gap, cfb, fbs A and fbs B (Suhermanto, Zairin, Lusiastuti, & Nuryati, 2019).…”

Section: Introductionmentioning

confidence: 99%

Maternal immunity of tilapia broodstock vaccinated with polyvalent vaccine and resistance of their offspring againstStreptococcus agalactiae

2020

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This study aimed to examine the use of Streptococcus agalactiae polyvalent vaccine in tilapia broodstock and the effect of maternal immunity and resistance on their offspring against S. agalactiae strain. The broodstock was injected with polyvalent vaccine of S. agalactiae at a dose of 108 CFU per fish at 2nd gonadal maturity until spawning. Challenge test was carried out on the offspring at the 5, 10, 15 and 20 days after hatching using NK1, N17O, N14G, N3M, N4M strain respectively and combination of them. We observed immunological parameters in broodstock, eggs and larvae and relative per cent survival (RPS) of larvae after challenged with pathogenic S. agalactiae. The results showed that the leukocytes, phagocytic activity, respiratory burst, lysozyme activity and antibody levels of vaccinated broodstock had higher level compared with unvaccinated broodstock. The high level of the lysozyme activity, antibody levels and recombination activating gene 1 (RAG1) were also observed in eggs and larvae from vaccinated broodstock. Larvae produced from vaccinated broodstock when challenged with variety strain of pathogenic S. agalactiae had RPS value more than 50% until 20 days after hatching. In conclusion, polyvalent vaccine of S. agalactiae administrated in the broodstock could enhance immunity in the broodstock and protect their offspring from pathogenic S. agalactiae.

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Validation of IgY for the diagnosis of Streptococcus agalactiae-caused endocarditis and bacterial meningitis in Nile tilapia (Oreochromis niloticus)

Cited by 20 publications

References 33 publications

Antimicrobial activity of RP-1 peptide conjugate with ferrocene group

Antimicrobial activity of RP-1 peptide conjugate with ferrocene group

Potential of mucoadhesive nanocapsules in drug release and toxicology in zebrafish

Maternal immunity of tilapia broodstock vaccinated with polyvalent vaccine and resistance of their offspring againstStreptococcus agalactiae

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