Validation of Intersection Absorption Spectrum for Simultaneous Determination of Betamethasone Valerate and Neomycin Sulfate in Cream

Abstract

Betamethasone valerate and neomycin sulfate are combinations of anti-infection in skin cream. The purpose of this study was to validate intersection absorption spectrum methods and simultaneous determination of betamethasone valerate and neomycin sulfate in the skin cream using 70% ethanol as solvent. The simultaneous determination of a sample was performed with the intersection point spectrophotometric methods when betamethasone valerate and neomycin sulfate absorption spectrum were overlapped and the intersection points were obtained at wavelength 245.4 nm. Each level of betamethasone valerate and neomycin sulfate were calculated using the regression equation. The simultaneous determination of betamethasone valerate and neomycin sulfate in cream supply obtained levels of (107,98 ± 1.6491)% betamethasone valerate and (94.81 ± 2.6958)% neomycin sulfate. The validation test for betamethasone valerate were as follows accuracy was 99.90%, precision was 1.09%, LOD was 0.645 µg/mL, and LOQ was 2.15 µg/mL whereas neomycin sulfate was as follows accuracy was 99.67%, precision was 1.46%, LOD was 2.215 µg/mL, and LOQ was 7.385 µg/mL. The mixture of betamethasone valerate and neomycin sulfate used a wavelength of 245.4 nm as the point of intersection of the absorption spectrum and the results met Indonesian pharmacopeia requirements and this method satisfied the validation requirements.

Formulation and Quantitative Analysis of Betamethasone Valerate and Neomycin Sulfate Cream by High Performance Liquid Chromatography and Spectrophotometry

“…Based on Indonesian pharmacopeia, the analysis on neomycin sulfate is using microbial testing [5]. Another study shown that absorption ration and chemometric spectrophotometry methods was valid for determination betamethasone valerate and neomycin sulfate in cream [8], [9]. Thus, the present study aimed to make a formula containing vitamin E and make a comparison of determine levels of betamethasone valerate and neomycin sulfate in creams using High Performance Liquid Chromatography and Spectrophotometry Area Under Curve methods.…”

Chemometric Calculation for Determination of Betamethasone and Neomycin Mixture in Cream Supply by Ultraviolet Spectrophotometry

“…Besides that, this technique is successfully applied to all analytical methods in spectrophotometry [4]. Spectrophotometry ratio absorbance and area under the curve are two valid modified to quantify BET and NEO in cream [5], [6].…”

Simultaneous Assay of Metformin and Glibenclamide in Combined Dosage Form by Mean Centered of Spectra Ratio Methods

“…[2][3] Assays of Metformin and Glibenclamide in a single form was determined with maximum absorption at wavelengths of 236 nm for Metformin and 229 nm for Glibenclamide by the ultraviolet spectrophotometry method and an HPLC methods. [4][5] Various studies have been published for quantitative determination methods for simultaneous assays of drug mixtures have been developed by researchers using the HPLC method 6 , RP-HPLC 7 , matric methods 8 , intersection absorption spectrum method 9 , UV-visible spectrophotometry. [10][11] The combinations of Metformin and Glibenclamide preparations have adjacent absorption and interfere with each other simultaneously assay with the classic spectrophotometry method.…”