Recently, the millimeter-wave band has been used in outdoor wireless communications to improve the frequency utilization efficiency. In this study, we propose a method to realize line-of-sight multiple-input multiple-output (LOS-MIMO) transmission independent of the transmitted distance using a two-dimensional (2-D) fixed antenna element arrangement, assuming millimeter-wave-band communication by small autonomous unmanned aerial vehicles. In conventional LOS-MIMO transmission with uniformly spaced antenna elements, the theoretical upper bound of the channel capacity is obtained, which decreases at a certain transmitted distance. Therefore, we focus on the fact that the propagation channel characteristics in pure LOS are geometrically determined by the transmitted distance, and consider the optimization of the arrangement of antenna elements. The element arrangement is fixed without using antenna selection from the viewpoint of system simplification. In addition, from the viewpoint of array size, we study the optimization corresponding to a 2-D element arrangement. The proposed method approximates the optimal arrangement from a vast number of 2-D element arrangements using a genetic algorithm. We evaluate the channel capacity characteristics of the proposed method by computer simulation and show that the characteristic degradation due to change in the transmitted distance in conventional LOS-MIMO transmission is improved. In addition, we evaluate a 4 × 4 MIMO transmission in an outdoor environment. The 4 × 4 MIMO propagation channel is also measured by an actual measurement equipment in the 66-GHz band and it is shown that the measured and calculated results agree with each other. INDEX TERMS Millimeter wave, unmanned aerial vehicle, line-of-sight multiple-input multiple-output, non-uniform antenna array, genetic algorithm.