Validation of Masimo Pronto 7 and Hemocue 201 for hemoglobin determination in children from 1 to 5 years of age

Abstract

ObjectiveEvaluate the accuracy and precision of Hemocue 201 (Hemocue) and Masimo Pronto 7 (Masimo) devices for measuring hemoglobin in epidemiological studies using having as a gold standard venous blood samples.Material and methodsIn a field sample of 148 children aged 1–5 years were subjects to measure Hemoglobin (Hb) using Masimo, and a capillary blood sample for Hemocue; and a venous blood sample for an automatic analyzer. We constructed regression models with no intercept, for precision and predictability; concordance correlations for accuracy and precision; Band and Altman limits of agreement, and thierarchical linear models for measurement system variance diagnostics.ResultsBoth devices under estaimated Hb concentartions compared with gold standard. Hemocue and Masimo devices have regression coefficients of 0.887 and 0.876 with predictability of 98.7% and 98.6% respectively. Concordance correlation coefficients for Hemocue was 0.18341, (p<0.001) and for Masimo 0.16638, (p<0.001). Bland and Altman showed a difference of −1.51 for Hemocue and −1.62 for Masimo. The three level hierarchical linear model shows that 57.9% of the total variance for Masimo came from repeated measure on same subjects.ConclusionsHemocue and Masimo measure less concentrations of Hb than the gold standard. The accuracy and presicion of Hb measurements with Hemocue and Masimo devices are very similar in the field. Devices should be used properly in field work, based on enhanced training schedules.