Validation of mating type-related markers in Ulva prolifera (Ulvophyceae, Chlorophyta) and their detection during various reproductive modes

Liu, Qianchun; Wu, Chunhui; Xie, Weifeng; Zhao, Jianmin; Jiang, Peng

doi:10.1016/j.algal.2021.102611

Cited by 4 publications

(2 citation statements)

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“…We attempted to induce maturation by culturing wild D. dichotoma under various culture conditions for evaluations of reproductive morphology; however, the thalli did not always become fertile (T. Arai & M. Kamiya, unpublished data). Recently, sex-specific molecular markers were developed for some isomorphic green and red macroalgae, such as U. prolifera (Liu et al 2022) (Guillemin et al 2012) and Gracilaria vermiculophylla (Ohmi) Papenfuss (as Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N. Norris & Fredericq;Krueger-Hadfield et al 2021), and the fecundity of each phase or sex was documented in a wild population using these markers (Vieira et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, sex‐specific molecular markers were developed for some isomorphic green and red macroalgae, such as U. prolifera (Liu et al . 2022), Gracilaria chilensis C.J.Bird, McLachlan & E.C.Oliveira (Guillemin et al . 2012) and Gracilaria vermiculophylla (Ohmi) Papenfuss (as Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N.Norris & Fredericq; Krueger‐Hadfield et al .…”

Section: Introductionmentioning

confidence: 99%

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Field survey of the phase and sex ratios of the brown alga Dictyota dichotoma (Dictyotales, Phaeophyceae) using sex‐specific molecular markers

Arai,

Koiwai,

Nozaki

et al. 2024

Phycological Research

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SUMMARYDominance of one phase (i.e. diploid or haploid) has been reported in various isomorphic macroalgae; however, studies of the relative frequencies of the two phases are limited to a few taxa owing to the difficulty in identifying phases of sterile thalli. In Dictyota dichotoma, we have previously documented the dominance of fertile sporophytes. In the present study, the phase and sex ratios of sterile thalli of D. dichotoma were determined using newly developed sex‐specific markers. Differentially expressed genes were detected by transcriptome analyses of female and male gametophytic strains and a sporophytic strain aiming to identify candidate sex‐specific genes. These candidates were aligned with genome sequences on the sex chromosomes of Ectocarpus siliculosus and were identified as putative sex‐specific genes of D. dichotoma. Two primer sets were designed to discriminate phase and sex by the presence or absence of PCR products, and these markers were applied to examine the phase and sex ratios of D. dichotoma in various habitats and seasons. The proportion of sporophytes against 33–50 thalli examined in each of the five habitats at Ebisu Island, Shizuoka Prefecture was 88–100%, and the proportion of fertile sporophytes against total sporophytes (fertility proportion) varied among the habitats (0–54%). The fertile sporophytes were remarkably larger than the sterile sporophytes. At Tateyama, Chiba Prefecture, the dominance of sporophytes occurred in all seasons. The fertility proportion of sporophyte was relatively high from February (62.4%) to May (91.9%) and lower than 23% in other seasons. When artificial substrates were anchored in the middle of the D. dichotoma population, newly recruited thalli were all sporophytes, suggesting infrequent reproduction via tetraspores in nature. Based on these findings, we discuss potential fitness differences between the phases of D. dichotoma.

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