Validation of quantitative trait loci for Fusarium head blight and kernel discoloration in barley

Canci, Paulo César; Nduulu, Lexingtons M.; Muehlbauer, Gary J.; Dill‐Macky, Ruth; Rasmusson, D. C.; Smith, Kevin P.

doi:10.1023/b:molb.0000037998.27661.58

Cited by 28 publications

(32 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, FHB resistance is partially correlated with plant height and heading date (de la Peña et al, 1999;Mesfi n et al, 2003;Massman et al, 2010). Quantitative trait loci (QTL) for FHB resistance and DON concentration often map to the same positions as plant height, heading date, and spike morphology QTL (Zhu et al, 1999;Canci et al, 2004;Lamb et al, 2009). Second, screening for these traits is laborious and expensive.…”

mentioning

confidence: 99%

Potential and Optimization of Genomic Selection for Fusarium Head Blight Resistance in Six‐Row Barley

2012

View full text Add to dashboard Cite

Fusarium head blight (FHB) is a devastating disease of barley {Hordeum vulgäre L.), causing reductions in yield and quality. Marker-based selection for resistance to FHB and lowered deoxynivalenol (DON) grain concentration would save considerable costs and time associated with phenotyping. A marker-based selection approach called genomic selection (GS) uses genomewide marker information to predict genetic value. We used a cross-validation approach that separated training sets from validation sets by both entry and environment. We used this framework to test the potential of GS for genetic improvement of FHB and DON as well as test the effect of different factors on prediction accuracy. Prediction accuracy for FHB was found to be as high as 0.72 and that for DON was found to be as high as 0.68. Little difference was found between marker effect estimation methods in terms of prediction of entry genetic value. The extensive linkage disequilibrium (LD) present in this population allowed the marker set to be reduced to 384 markers and training population (TP) size to be reduced 200 with little effect on prediction accuracy. We found little to no advantage to combining subpopulations that correspond to neighboring breeding programs to increase TP size. Apparently, little genetic information is shared between subpopulations, either because of different marker-quantitative trait loci (OTL) linkage phases, different segregating OTL, or nonadditive gene action.

show abstract

mentioning

confidence: 99%

Potential and Optimization of Genomic Selection for Fusarium Head Blight Resistance in Six‐Row Barley

2012

View full text Add to dashboard Cite

show abstract

“…Most of the lines with resistance are not adapted to the US barleygrowing regions and lack agronomic and quality traits needed for US cultivars (Urrea et al 2005). Quantitative trait loci (QTL) for resistance to FHB and DON have been located on all seven barley chromosomes (de la Pena et al 1999, Zhu et al 1999, Ma et al 2000, Dahleen et al 2003, Canci et al 2004, Hori et al 2005, Horsley et al 2006, Nduulu et al 2007, Sato et al 2008, Yu et al 2010 using simple interval mapping and composite interval mapping (CIM).…”

mentioning

confidence: 99%

Quantitative trait loci (QTL) for Fusarium ELISA compared to QTL for Fusarium head blight resistance and deoxynivalenol content in barley

et al. 2012

View full text Add to dashboard Cite

Fusarium head blight (FHB) and deoxynivalenol (DON) mycotoxin produced by Fusarium graminearum reduce barley yield and quality worldwide. An enzyme-linked immunosorbent assay (ELISA) using an antibody specific to Fusarium was proposed as an alternative for measuring FHB instead of counting infected kernels per spike. Cleistogamy (closed flowering) may be an avoidance mechanism for FHB resistance. This study was conducted to determine whether quantitative trait loci (QTL) for Fusarium ELISA were colocated with QTL for FHB, DON, heading date, height and spike density and/or the gene for cleistogamy. Doubled haploid lines from Zhedar 2/ ND9712//Foster were tested in ten environments and used to develop a 260-marker linkage map. QTL analysis located 24 significant regions for the six traits. The effect of cleistogamy on resistance was unclear and environment specific. Of the two QTL located for ELISA, only one corresponded with a QTL for FHB, but large allelic differences in ELISA were found for the regions significantly associated with FHB and DON, indicating that ELISA could be a simpler method to identify lines with FHB resistance and low DON.

show abstract

“…Study has been carried to find QTLs associated with FHB resistance using SSR markers (Canci et al 2004). Only indirect association with FHB might be thus found.…”

Section: Resultsmentioning

confidence: 99%

The use of molecular markers for characterisation of spring barley for breeding to Fusarium head blight resistance

Špunarová¹,

Ovesná²,

Tvarůžek³

et al. 2005

PLANT SOIL ENVIRON

View full text Add to dashboard Cite

Fusarium head blight (FHB) is a barley disease, which occurs every year in various areas of barley cultivation all over the world and the increasing incidence has been confirmed in the Czech Republic also during the last years. We aimed to emply AFLP (Amplified fragment length polymorphism) and SSR (Single sequence repeats) markers to describe diversity among breeding lines with a sufficient level of resistance towards FHB and to find marker(s) associated with the analysed traits. The number of eight accessions including five expected resistance donors and three sensitive lines were tested in the field and laboratory. The field values and the amount of deoxynivalenol were positively correlated (r = 0.92). The laboratory test and content DON manifested also a high correlation (r = 0.73). Several DH lines developed from androgenetic barley progenies of the F1 hybrids between the susceptible line PI 383933 and resistant line PEC 210 or the susceptible line PI 383933 and resistant cultivar Chevron were found resistant towards Fusarium infection in both the field and laboratory tests. Low infestation was found at line DH 37 from combination Chevron × PI 383933 and lines DH48, DH49, DH50 and DH55 from the combination PEC 210 × PI 383933. Cluster analyses based on 68 AFLP and 18 SSR markers demonstrate a genetic relationship among parental genotypes and DH lines. Some DH lines combined a sufficient degree of resistance against FHB and extract content (basic parameters malting quality). Statistically significant differences in malt-extract values were observed between groups of the DH lines possessing and not possessing the AFLP marker CAA/AGC 341bp. The markers will be further evaluated and optionally used for MAS.

show abstract

Validation of quantitative trait loci for Fusarium head blight and kernel discoloration in barley

Cited by 28 publications

References 26 publications

Potential and Optimization of Genomic Selection for Fusarium Head Blight Resistance in Six‐Row Barley

Potential and Optimization of Genomic Selection for Fusarium Head Blight Resistance in Six‐Row Barley

Quantitative trait loci (QTL) for Fusarium ELISA compared to QTL for Fusarium head blight resistance and deoxynivalenol content in barley

The use of molecular markers for characterisation of spring barley for breeding to Fusarium head blight resistance

Contact Info

Product

Resources

About