Validation of the AmpFℓSTR™ Profiler Plus PCR Amplification Kit for Use in Forensic Casework

Frank, William E.; Llewellyn, Barbara; Fish, Pamela A.; Riech, Angela K.; Marcacci, Tabithah L.; Gandor, Daniel W.; Parker, Donald E.; Carter, Rhonda R.; Thibault, Sarah M.

doi:10.1520/jfs15017j

Cited by 30 publications

(15 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The precision evaluation was performed as described previously [5,10,11]. DNA sequencing was performed for novel STRs, novel alleles, off-ladder alleles, and loci with limited sequence data using methods described previously [5].…”

Section: Methodsmentioning

confidence: 99%

Fifteen non-CODIS autosomal short tandem repeat loci multiplex data from nine population groups living in Taiwan

et al. 2012

View full text Add to dashboard Cite

The analysis of autosomal short tandem repeat (STR) loci is a powerful tool in forensic genetics. We developed a multiplex system in which 15 non-Combined DNA Index System autosomal STRs (D3S1744, D4S2366, D8S1110, D10S2325, D12S1090, D13S765, D14S608, Penta E, D17S1294, D18S536, D18S1270, D20S470, D21S1437, Penta D, and D22S683) could be amplified in one single polymerase chain reaction. DNA samples from 1,098 unrelated subjects of nine population groups living in Taiwan, including Taiwanese Han, indigenous Taiwanese of Taiwan Island, Tao, mainland Chinese, Filipinos, Thais, Vietnamese, Indonesians, and Caucasians, were collected and analyzed using this system. The distributions of the allelic frequencies and the forensic parameters of each population group were presented. The combined discrimination power and the combined power of exclusion were high in all population groups tested in this study. A multidimensional scaling plot of these nine population groups based on the Reynolds' genetic distances calculated from 15 autosomal STRs was constructed, and the genetic substructure in this area was presented. In conclusion, this 15 autosomal STR multiplex system provides highly informative STR data and appears useful in forensic casework and parentage testing in different populations.

show abstract

Section: Methodsmentioning

confidence: 99%

Fifteen non-CODIS autosomal short tandem repeat loci multiplex data from nine population groups living in Taiwan

et al. 2012

View full text Add to dashboard Cite

show abstract

“…STR typing typically involves a polymerase chain reaction (PCR) amplification step followed by size fractionation of the resulting products and fluorescent signal detection and processing. Numerous studies have shown that the signal associated with each allele of a heterozygote at a given locus is approximately equal [3][4][5][6][7][8][9]. General practice has shown that the peak height ratio, as measured by dividing the height (in relative fluorescent units (RFUs)) of the lower quantity allele by the height of the higher quantity allele "should be greater than approximately 70% in a single source sample" [3].…”

Section: Introductionmentioning

confidence: 99%

Magnitude-dependent variation in peak height balance at heterozygous STR loci

Gilder¹,

Inman

Shields

et al. 2010

Int J Legal Med

View full text Add to dashboard Cite

When the smaller of two peaks at an STR locus is less than 70% the magnitude of the larger peak at that locus, the disparity is typically taken to be an indication that there is more than one contributor of template DNA to the sample being tested. An analysis of 1,763 heterozygous allele pairs suggests that a peak height imbalance threshold that varies with the magnitude of the peaks being evaluated at a locus is superior to a fixed threshold. Identifying samples that are likely to be mixtures and those that are likely to have arisen from a single source is accomplished more reliably when a statistically based, magnitude-dependent peak height imbalance threshold is used. The amelogenin locus was found to behave in a similar fashion and was also found to have no systematic bias that favored the amplification of Y or X alleles.

show abstract

“…The precision of PCR fragments were assessed using three selected donor DNA samples (A, B, and C) and three control DNA samples (9947A, GM9948, and GM3657) among each locus especially those that exhibited the following characteristics: (1) the shortest fragment (102 base, D21S1437, allele 7) and the longest fragment (455 base, Penta D, allele 18), (2) alleles that differ in size by one or two bases (D18S1270, alleles 9.1 and 9.2; D22S683, alleles13.2, 14, and 14.2), and (3) "n" and "n±1" alleles. Precision was calculated as the standard deviation (SD) of size estimated as described previously [16,17].The bins for the GeneMapper software and the category boundaries for the Genotyper were derived from the resulted SD.…”

Section: Methodsmentioning

confidence: 99%

Fourteen non-CODIS autosomal short tandem repeat loci multiplex data from Taiwanese

Hwa

Chang²,

Lee

et al. 2010

Int J Legal Med

View full text Add to dashboard Cite

Interest in the development of polymorphic short tandem repeat (STR) markers unlinked to the CODIS loci is growing among forensic practitioners. We developed a multiplex system in which14 autosomal STR (D3S1744, D4S2366, D8S1110, D12S1090, D13S765, D14S608, Penta E, D17S1294, D18S536, D18S1270, D20S470, D21S1437, Penta D, and D22S683) could be amplified in one single polymerase chain reaction. DNA samples from 572 unrelated Taiwanese Han subjects were analyzed using this 14 STR multiplex system. Thirty parent-child pairs of parentage testing cases with a combined paternity index (CPI) below 1,000 and 32 parent-child pairs with single-step mutations found in AmpFℓSTR Identifiler loci were also recruited for validation of the newly developed system. DNA sequencing was performed for novel STRs and novel alleles found in these subjects. The distributions of allelic frequencies for these autosomal STRs and sequence data, allele nomenclature for the STRs, and forensic parameters are presented. The discrimination power in our multiplex loci ranged from 0.6858 (D18S536) to 0.9168 (Penta E), with a combined discrimination power of 0.999999999. It provides additional power to distinguish the possible single-step mutations in parent-child pairs and improves the ability to prove parentage by increasing the CPI. The combined power of exclusion of these 14 loci in Taiwanese Han in this study was 0.9999995913. In conclusion, this 14-autosomal STRs multiplex system provides highly informative STR data and appears useful in forensic casework and parentage testing.

show abstract

Validation of the AmpFℓSTR™ Profiler Plus PCR Amplification Kit for Use in Forensic Casework

Cited by 30 publications

References 8 publications

Fifteen non-CODIS autosomal short tandem repeat loci multiplex data from nine population groups living in Taiwan

Fifteen non-CODIS autosomal short tandem repeat loci multiplex data from nine population groups living in Taiwan

Magnitude-dependent variation in peak height balance at heterozygous STR loci

Fourteen non-CODIS autosomal short tandem repeat loci multiplex data from Taiwanese

Contact Info

Product

Resources

About