Validation of the G1 Molecular Marker Associated with Resistance to Orange Rust in Brazilian Sugarcane Genotypes

Hoepers, Lívia Maria Lemos; Koch, Gabriel; Silva, Mayara Catherine Candido; Ruaro, Lucimeris; Calegario, Renata Faier; Filho, João Carlos Bespalhok; Oliveira, Ricardo Augusto de; Duarte, Henrique da Silva Silveira

doi:10.1007/s12355-020-00896-x

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…Hoepers et al [ 72 ] evaluated 80 sugarcane genotypes for the presence of the G1 marker and concluded that the marker had low efficiency for predicting resistance to orange rust in clones selected in a genetic improvement program. On the other hand, Fier et al [ 32 ] observed high efficiency in predicting resistance using the G1 marker (71.43%) when evaluating 24 Brazilian commercial cultivars.…”

Section: Discussionmentioning

confidence: 99%

Molecular evaluation and phenotypic screening of brown and orange rust in Saccharum germplasm

Oliveira,

Barreto,

Balsalobre

et al. 2024

PLoS ONE

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Brazil is the largest global producer of sugarcane and plays a significant role—supplier of sugar and bioethanol. However, diseases such as brown and orange rust cause substantial yield reductions and economic losses, due decrease photosynthesis and biomass in susceptible cultivars. Molecular markers associated with resistance genes, such as Bru1 (brown rust) and G1 (orange rust), could aid in predicting resistant genotypes. In this study, we sought to associate the phenotypic response of 300 sugarcane accessions with the genotypic response of Bru1 and G1 markers. The field trials were conducted in a randomized block design, and five six-month-old plants per plot were evaluated under natural disease conditions. Genotypic information about the presence or absence of Bru1 (haplotype 1) and G1 gene was obtained after extraction of genomic DNA and conventional PCR. Of the total accessions evaluated, 60.3% (181) showed resistance to brown rust in the field, and of these, 70.7% (128) had the Bru1 gene present. Considering the field-resistant accessions obtained from Brazilian breeding programs (116), the Bru1 was present in 77,6% of these accessions. While alternative resistance sources may exist, Bru1 likely confers enduring genetic resistance in current Brazilian cultivars. Regarding the phenotypic reaction to orange rust, the majority of accessions, 96.3% (288), were field resistant, and of these, 52.7% (152) carried the G1 marker. Although less efficient for predicting resistance when compared to Bru1, the G1 marker could be part of a quantitative approach when new orange rust resistance genes are described. Therefore, these findings showed the importance of Bru1 molecular markers for the early selection of resistant genotypes to brown rust by genetic breeding programs.

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Section: Discussionmentioning

confidence: 99%

Molecular evaluation and phenotypic screening of brown and orange rust in Saccharum germplasm

Oliveira,

Barreto,

Balsalobre

et al. 2024

PLoS ONE

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“…In the work by Fier et al (2020), using the molecular marker G1 in Brazilian sugarcane cultivars, observed an efficiency in the prediction of resistance of 71.43%, indicating that this marker could be used for assisted selection in sugarcane breeding programs. In a study carried out by Hoepers et al (2020), the authors did not validate the molecular marker G1 because it showed an accuracy of 22.86% in the prediction of the phenotype resistant to orange rust, using clones selected in a Brazilian breeding program and commercial sugarcane cultivars. For the group of parents used in this study, the molecular marker G1 showed an accuracy of 71% in the prediction of resistant parents, a result similar to that obtained by Yang et al (2018) and Fier et al (2020) and higher than those obtained by Hoepers et al (2020).…”

Section: Discussionmentioning

confidence: 99%

“…The G1 molecular marker was the first marker to be published associated with the resistance of sugarcane to orange rust and showed an efficiency of 65.8% in the prediction of resistant phenotypes in mapping studies of a F1 segregating population obtained from the cross between clones CP95-1039 (resistant) and CP88-1762 (susceptible) (Yang et al, 2018). Fier et al (2020) reported an evaluation efficiency in predicting resistance to orange rust of 71.43% using the molecular marker G1 when evaluating Brazilian commercial cultivars, whereas Hoepers et al (2020) observed an efficiency in predicting the resistant phenotype of 22.86% using clones in the selection phase and commercial cultivars.…”

Section: Introductionmentioning

confidence: 99%

Research Article Resistance to orange rust associated with the G1 molecular marker in parents of Brazilian RB sugarcane varieties

Borella¹,

Brasileiro²,

Azeredo³

et al. 2022

Genet. Mol. Res.

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RB867515 (resistant to orange rust). Of the 63 sugarcane parents, 43 were classified as resistant, of which 27 were positive for the G1 marker; 10 as intermediate, including seven positives for G1; 10 were susceptible, among which four were positive for this marker. The molecular marker G1 showed an accuracy of 71% in predicting the resistant phenotype and could be used as a tool for the characterization of resistant germplasm.

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Validation of the G1 Molecular Marker Associated with Resistance to Orange Rust in Brazilian Sugarcane Genotypes

Cited by 2 publications

References 21 publications

Molecular evaluation and phenotypic screening of brown and orange rust in Saccharum germplasm

Molecular evaluation and phenotypic screening of brown and orange rust in Saccharum germplasm

Research Article Resistance to orange rust associated with the G1 molecular marker in parents of Brazilian RB sugarcane varieties

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