2021
DOI: 10.3390/pathogens10070864
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Validation of the Use of Dried Blood Samples for the Detection of Toxoplasma gondii Antibodies in Stray Cats (Felis s. catus)

Abstract: If validated beforehand, the analysis of dried blood on blotting paper (BP samples) is very useful for monitoring free-ranging animals. We aimed to validate this method for the detection of antibodies against Toxoplasma gondii in stray cats. We used the modified agglutination test (MAT) in 199 sample pairs of sera and BP samples from 54, 39, 56, and 50 cats trapped during four periods in five dairy farms. Screening was at 1:6, 1:12, and 1:24 dilutions. The cut-off value was at MAT titre ≥ 24, but MAT titre ≥ 1… Show more

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Cited by 2 publications
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“…Serodiagnosis of T. gondii in stray cats may be challenging due to difficulties with sample collection. Simon et al [12] validated the use of dried blood samples for the detection of T. gondii antibodies in stray cats, as an easy and cheap alternative to classical blood collection and serum separation before examination. The results indicate that applying the modified agglutination test to blood dried on blotting paper is reliable for identifying the presence of T. gondii antibodies in domestic cats, offering a practical technique for monitoring free-ranging animals [12].…”
Section: New Insights In Classical and Lesser Studied Feline Parasitesmentioning
confidence: 99%
“…Serodiagnosis of T. gondii in stray cats may be challenging due to difficulties with sample collection. Simon et al [12] validated the use of dried blood samples for the detection of T. gondii antibodies in stray cats, as an easy and cheap alternative to classical blood collection and serum separation before examination. The results indicate that applying the modified agglutination test to blood dried on blotting paper is reliable for identifying the presence of T. gondii antibodies in domestic cats, offering a practical technique for monitoring free-ranging animals [12].…”
Section: New Insights In Classical and Lesser Studied Feline Parasitesmentioning
confidence: 99%