VALITEST: Validation of diagnostic tests to support plant health

Abstract

Consiglio per la ricerca in agricoltura e l'analisi dell'economia agrarian (CREA),

PM 7/20 (3) Erwinia amylovora

“…In the test performance study performed in the framework of VALITEST (Alič et al, 2020;Trontin et al, 2021), the following DNA extraction kits were used by several participants that reported valid results: QuickPick TM SML Plant DNA Kit (Bio-Nobile), DNeasy Plant Mini Kit (Qiagen) and DNeasy Mericon Food kit (Qiagen). The following were used by one participant and led to production of valid data: Mericon Bacteria Kit (Qiagen), NucleoSpin Plant II (Macherey-Nagel), E.Z.N.A.…”

“…The two primer pairs were evaluated in the test performance study organized within the framework of the VALITEST project (Alič et al, 2020;Trontin et al, 2021)…”

“…The accuracy in the 2010 test performance study could not be tested with this real-time PCR; however, it was tested by one laboratory in parallel with the realtime PCR described in Pirc et al (2009) and gave the same qualitative results with the DNA extraction from Llop et al (1999) protocol. This test was further evaluated in the test performance studies organized within the framework of the VALITEST project (Alič et al, 2020;Trontin et al, 2021) product). For PCRs not performed on bacterial colonies, the PAC should preferably be near the limit of detection.…”

“…All tests were evaluated in test performance studies in 2009 (Reisenzein et al, 2010), 2010 (López et al, 2010) and/or 2019 (Alič et al, 2020; Trontin et al, 2021), and are recommended for the analyses of organs with symptoms after a DNA extraction step. The DNA extraction protocols that were evaluated in a test performance study in 2009 and/or 2019 are indicated in Appendix .…”

Guidelines for improving statistical analyses of validation datasets for plant pest diagnostic tests

“…the determination of its performance characteristics, is essential before it can be implemented in plant pest diagnostics. Validation provides essential information on the performance of a test, supports the reliability of the diagnostic activity and assists in the selection of a test that is appropriate for its intended use (Trontin et al, 2021). Test validation can be carried out within a laboratory (intra‐laboratory) or in the framework of inter‐laboratory comparison studies by several laboratories (such as proficiency testing or test performance study, TPS).…”